Plasma membrane disruption in orthodontic tooth movement in rats

M. F. Orellana, A. K. Smith, Jennifer L Waller, Eladio DeLeon, J. L. Borke

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sublethal plasma membrane disruption (PMD) is an established mechanism for signaling in several cell types, including endothelial cells and skeletal muscle. We used a rat model of orthodontic tooth movement to test the hypothesis that periodontal ligament (PDL) cells communicate stretch to changes in bone cell activity in part via PMD. To produce PMD, we used a 50-g load from a spring activated in the buccal direction against the maxillary first molars for 5 min. Uptake of endogenous serum albumin was used as a PMD marker. Immunohistochemistry demonstrates albumin in PDL cells surrounding moved first molar tips. Image analysis shows significantly more albumin in cells of the buccal side (tension) of the moved teeth compared with those of the lingual, distal, and mesial sides, and those of the unmoved control. Albumin localization within cells of the PDL, after only 5 min of mechanical loading, suggests that PMD could promote uptake or release of signaling molecules.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalJournal of Dental Research
Volume81
Issue number1
DOIs
StatePublished - Jan 1 2002

Fingerprint

Tooth Movement Techniques
Cell Membrane
Periodontal Ligament
Albumins
Cheek
Tongue
Serum Albumin
Tooth
Skeletal Muscle
Endothelial Cells
Immunohistochemistry
Bone and Bones

Keywords

  • Mechanotransduction
  • Orthodontic tooth movement
  • Periodontal ligament
  • Plasma membrane disruption

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Plasma membrane disruption in orthodontic tooth movement in rats. / Orellana, M. F.; Smith, A. K.; Waller, Jennifer L; DeLeon, Eladio; Borke, J. L.

In: Journal of Dental Research, Vol. 81, No. 1, 01.01.2002, p. 43-47.

Research output: Contribution to journalArticle

Orellana, M. F. ; Smith, A. K. ; Waller, Jennifer L ; DeLeon, Eladio ; Borke, J. L. / Plasma membrane disruption in orthodontic tooth movement in rats. In: Journal of Dental Research. 2002 ; Vol. 81, No. 1. pp. 43-47.
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