Plasma membrane phospholipid and cholesterol distribution of skin fibroblasts from drug-naive patients at the onset of psychosis

Sahebarao P. Mahadik, Sukdeb Mukherjee, Elizabeth E. Correnti, Hemant S. Kelkar, Chandramohan G. Wakade, Richard M. Costa, R. Scheffer

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Contents of plasma membrane major phospholipids, cholesterol, and cholesteryl esters of fibroblasts from drug-naive psychotic patients were compared with those from normal controls. Total membrane lipids were extracted and individual lipids were separated on high-performance thin-layer chromatography. The contents of lipid bands were quantitated by densitometric scanning and comparing with standards. Contents of total phospholipids as well as phosphatidylserine, phosphatidylinositol and phosphatidylethanolamine were significantly lower in fibroblasts from patients than in those from normal controls (P<0.001, <0.005, <0.05, <0.05 respectively). Total cholesterol fraction and cholesteryl esters were also significantly lower in fibroblasts from patient (P <0.005, <0.001 respectively). These changes were not related to differences in age or sex. These data support the hypothesis that schizophrenia is associated with disordered membrane lipid metabolism, and that this predates the onset of psychosis.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalSchizophrenia Research
Volume13
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Cholesterol Esters
Psychotic Disorders
Phospholipids
Fibroblasts
Cholesterol
Cell Membrane
Skin
Membrane Lipids
Pharmaceutical Preparations
Lipids
Phosphatidylserines
Thin Layer Chromatography
Prednisolone
Phosphatidylinositols
Lipid Metabolism
Schizophrenia

Keywords

  • Cholesterol
  • Fibroblast
  • Plasma membrane phospholipid
  • Psychosis

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry

Cite this

Plasma membrane phospholipid and cholesterol distribution of skin fibroblasts from drug-naive patients at the onset of psychosis. / Mahadik, Sahebarao P.; Mukherjee, Sukdeb; Correnti, Elizabeth E.; Kelkar, Hemant S.; Wakade, Chandramohan G.; Costa, Richard M.; Scheffer, R.

In: Schizophrenia Research, Vol. 13, No. 3, 01.01.1994, p. 239-247.

Research output: Contribution to journalArticle

Mahadik, Sahebarao P. ; Mukherjee, Sukdeb ; Correnti, Elizabeth E. ; Kelkar, Hemant S. ; Wakade, Chandramohan G. ; Costa, Richard M. ; Scheffer, R. / Plasma membrane phospholipid and cholesterol distribution of skin fibroblasts from drug-naive patients at the onset of psychosis. In: Schizophrenia Research. 1994 ; Vol. 13, No. 3. pp. 239-247.
@article{67c137d7f4c74346b3eb6b7528f4c803,
title = "Plasma membrane phospholipid and cholesterol distribution of skin fibroblasts from drug-naive patients at the onset of psychosis",
abstract = "Contents of plasma membrane major phospholipids, cholesterol, and cholesteryl esters of fibroblasts from drug-naive psychotic patients were compared with those from normal controls. Total membrane lipids were extracted and individual lipids were separated on high-performance thin-layer chromatography. The contents of lipid bands were quantitated by densitometric scanning and comparing with standards. Contents of total phospholipids as well as phosphatidylserine, phosphatidylinositol and phosphatidylethanolamine were significantly lower in fibroblasts from patients than in those from normal controls (P<0.001, <0.005, <0.05, <0.05 respectively). Total cholesterol fraction and cholesteryl esters were also significantly lower in fibroblasts from patient (P <0.005, <0.001 respectively). These changes were not related to differences in age or sex. These data support the hypothesis that schizophrenia is associated with disordered membrane lipid metabolism, and that this predates the onset of psychosis.",
keywords = "Cholesterol, Fibroblast, Plasma membrane phospholipid, Psychosis",
author = "Mahadik, {Sahebarao P.} and Sukdeb Mukherjee and Correnti, {Elizabeth E.} and Kelkar, {Hemant S.} and Wakade, {Chandramohan G.} and Costa, {Richard M.} and R. Scheffer",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/0920-9964(94)90048-5",
language = "English (US)",
volume = "13",
pages = "239--247",
journal = "Schizophrenia Research",
issn = "0920-9964",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Plasma membrane phospholipid and cholesterol distribution of skin fibroblasts from drug-naive patients at the onset of psychosis

AU - Mahadik, Sahebarao P.

AU - Mukherjee, Sukdeb

AU - Correnti, Elizabeth E.

AU - Kelkar, Hemant S.

AU - Wakade, Chandramohan G.

AU - Costa, Richard M.

AU - Scheffer, R.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Contents of plasma membrane major phospholipids, cholesterol, and cholesteryl esters of fibroblasts from drug-naive psychotic patients were compared with those from normal controls. Total membrane lipids were extracted and individual lipids were separated on high-performance thin-layer chromatography. The contents of lipid bands were quantitated by densitometric scanning and comparing with standards. Contents of total phospholipids as well as phosphatidylserine, phosphatidylinositol and phosphatidylethanolamine were significantly lower in fibroblasts from patients than in those from normal controls (P<0.001, <0.005, <0.05, <0.05 respectively). Total cholesterol fraction and cholesteryl esters were also significantly lower in fibroblasts from patient (P <0.005, <0.001 respectively). These changes were not related to differences in age or sex. These data support the hypothesis that schizophrenia is associated with disordered membrane lipid metabolism, and that this predates the onset of psychosis.

AB - Contents of plasma membrane major phospholipids, cholesterol, and cholesteryl esters of fibroblasts from drug-naive psychotic patients were compared with those from normal controls. Total membrane lipids were extracted and individual lipids were separated on high-performance thin-layer chromatography. The contents of lipid bands were quantitated by densitometric scanning and comparing with standards. Contents of total phospholipids as well as phosphatidylserine, phosphatidylinositol and phosphatidylethanolamine were significantly lower in fibroblasts from patients than in those from normal controls (P<0.001, <0.005, <0.05, <0.05 respectively). Total cholesterol fraction and cholesteryl esters were also significantly lower in fibroblasts from patient (P <0.005, <0.001 respectively). These changes were not related to differences in age or sex. These data support the hypothesis that schizophrenia is associated with disordered membrane lipid metabolism, and that this predates the onset of psychosis.

KW - Cholesterol

KW - Fibroblast

KW - Plasma membrane phospholipid

KW - Psychosis

UR - http://www.scopus.com/inward/record.url?scp=0027978180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027978180&partnerID=8YFLogxK

U2 - 10.1016/0920-9964(94)90048-5

DO - 10.1016/0920-9964(94)90048-5

M3 - Article

VL - 13

SP - 239

EP - 247

JO - Schizophrenia Research

JF - Schizophrenia Research

SN - 0920-9964

IS - 3

ER -