Platelet-activating factor-induced inositol 1,4,5-trisphosphate generation in undifferentiated and differentiated U937 cells: Role of tyrosine kinase

Worku Abebe, Nawab Ali, Devendra K. Agrawal

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We compared the effect of platelet-activating factor (PAF) on inositol 1,4,5-trisphosphate (IP3) content in undifferentiated and differentiated U937 cells. In both cell types, PAF induced a rapid, transient and concentration-dependent elevation of IP3 content. The production of IP3 in response to PAF was greater in differentiated than in undifferentiated cells. The increases in IP3 produced by PAF in both types of cell were inhibited by the PAF receptor antagonist, WEB 2086, as well as by the tyrosine kinase inhibitor, genistein. PAF also caused increased tyrosine phosphorylation of a 32 kDa protein substrate in both undifferentiated and differentiated cells. The magnitude of the phosphorylation was, however, greater in tile differentiated cells. Genistein reduced the PAF-induced tyrosine phosphorylation of the substrate in both cell preparations. The specific binding of[3H]PAF was also markedly enhanced in differentiated cells. This effect was attenuated by genistein. The results indicate that PAF induces the production of IP3 in U937 cells via tyrosine kinase-mediated mechanisms and this process is augmented in differentiated cells.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalInternational Journal of Immunopharmacology
Volume18
Issue number3
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

U937 Cells
Inositol 1,4,5-Trisphosphate
Platelet Activating Factor
Protein-Tyrosine Kinases
Genistein
Phosphorylation
WEB 2086
Tyrosine

Keywords

  • 1,4,5-trisphosphate
  • Differentiation
  • Inositol
  • Platelet-activating factor
  • Tyrosine kinase
  • U937 cells

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

@article{bdfe445e0ba94793b8a97ae5eaa34cef,
title = "Platelet-activating factor-induced inositol 1,4,5-trisphosphate generation in undifferentiated and differentiated U937 cells: Role of tyrosine kinase",
abstract = "We compared the effect of platelet-activating factor (PAF) on inositol 1,4,5-trisphosphate (IP3) content in undifferentiated and differentiated U937 cells. In both cell types, PAF induced a rapid, transient and concentration-dependent elevation of IP3 content. The production of IP3 in response to PAF was greater in differentiated than in undifferentiated cells. The increases in IP3 produced by PAF in both types of cell were inhibited by the PAF receptor antagonist, WEB 2086, as well as by the tyrosine kinase inhibitor, genistein. PAF also caused increased tyrosine phosphorylation of a 32 kDa protein substrate in both undifferentiated and differentiated cells. The magnitude of the phosphorylation was, however, greater in tile differentiated cells. Genistein reduced the PAF-induced tyrosine phosphorylation of the substrate in both cell preparations. The specific binding of[3H]PAF was also markedly enhanced in differentiated cells. This effect was attenuated by genistein. The results indicate that PAF induces the production of IP3 in U937 cells via tyrosine kinase-mediated mechanisms and this process is augmented in differentiated cells.",
keywords = "1,4,5-trisphosphate, Differentiation, Inositol, Platelet-activating factor, Tyrosine kinase, U937 cells",
author = "Worku Abebe and Nawab Ali and Agrawal, {Devendra K.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/0192-0561(96)00004-5",
language = "English (US)",
volume = "18",
pages = "173--177",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Platelet-activating factor-induced inositol 1,4,5-trisphosphate generation in undifferentiated and differentiated U937 cells

T2 - Role of tyrosine kinase

AU - Abebe, Worku

AU - Ali, Nawab

AU - Agrawal, Devendra K.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - We compared the effect of platelet-activating factor (PAF) on inositol 1,4,5-trisphosphate (IP3) content in undifferentiated and differentiated U937 cells. In both cell types, PAF induced a rapid, transient and concentration-dependent elevation of IP3 content. The production of IP3 in response to PAF was greater in differentiated than in undifferentiated cells. The increases in IP3 produced by PAF in both types of cell were inhibited by the PAF receptor antagonist, WEB 2086, as well as by the tyrosine kinase inhibitor, genistein. PAF also caused increased tyrosine phosphorylation of a 32 kDa protein substrate in both undifferentiated and differentiated cells. The magnitude of the phosphorylation was, however, greater in tile differentiated cells. Genistein reduced the PAF-induced tyrosine phosphorylation of the substrate in both cell preparations. The specific binding of[3H]PAF was also markedly enhanced in differentiated cells. This effect was attenuated by genistein. The results indicate that PAF induces the production of IP3 in U937 cells via tyrosine kinase-mediated mechanisms and this process is augmented in differentiated cells.

AB - We compared the effect of platelet-activating factor (PAF) on inositol 1,4,5-trisphosphate (IP3) content in undifferentiated and differentiated U937 cells. In both cell types, PAF induced a rapid, transient and concentration-dependent elevation of IP3 content. The production of IP3 in response to PAF was greater in differentiated than in undifferentiated cells. The increases in IP3 produced by PAF in both types of cell were inhibited by the PAF receptor antagonist, WEB 2086, as well as by the tyrosine kinase inhibitor, genistein. PAF also caused increased tyrosine phosphorylation of a 32 kDa protein substrate in both undifferentiated and differentiated cells. The magnitude of the phosphorylation was, however, greater in tile differentiated cells. Genistein reduced the PAF-induced tyrosine phosphorylation of the substrate in both cell preparations. The specific binding of[3H]PAF was also markedly enhanced in differentiated cells. This effect was attenuated by genistein. The results indicate that PAF induces the production of IP3 in U937 cells via tyrosine kinase-mediated mechanisms and this process is augmented in differentiated cells.

KW - 1,4,5-trisphosphate

KW - Differentiation

KW - Inositol

KW - Platelet-activating factor

KW - Tyrosine kinase

KW - U937 cells

UR - http://www.scopus.com/inward/record.url?scp=0029891792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029891792&partnerID=8YFLogxK

U2 - 10.1016/0192-0561(96)00004-5

DO - 10.1016/0192-0561(96)00004-5

M3 - Article

C2 - 8796445

AN - SCOPUS:0029891792

VL - 18

SP - 173

EP - 177

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 3

ER -