Platelet dense-granule secretion plays a critical role in thrombosis and subsequent vascular remodeling in atherosclerotic mice

Sarah M. King, Rachel A. McNamee, Aiilyan K. Houng, Rakesh Patel, Michael Brands, Guy L. Reed

Research output: Contribution to journalArticle

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Abstract

Background-Platelet aggregation plays a critical role in myocardial infarction and stroke; however, the role of platelet secretion in atherosclerotic vascular disease is poorly understood. Therefore, we examined the hypothesis that platelet dense-granule secretion modulates thrombosis, inflammation, and atherosclerotic vascular remodeling after injury. Methods and Results-Functional deletion of the Hermansky-Pudlak syndrome 3 gene (HPS 3 -/-) markedly reduces platelet dense-granule secretion. HPS3 -/- mice have normal platelet counts, platelet morphology, and a-granule number, as well as maximal secretion of the a-granule marker P-selectin; however, their capacity to form platelet-leukocyte aggregates is significantly reduced (P<0.05). To examine the role of platelet dense-granule secretion in these processes, atherosclerosis-prone mice with combined genetic deficiency of apolipoprotein E and HPS3 (ApoE -/-, HPS3 -/-) were compared with congenie, atherosclerosis-prone mice with normal platelet secretion (ApoE -/-, HPS3 +/+). After 16 to 18 weeks on a high-fat diet, both groups of mice had similar fasting cholesterol levels and body weight. Carotid arteries of ApoE -/-, HPS3 +/+ mice thrombosed rapidly after FeCl 3 injury, but ApoE -/-, HPS3 -/- mice were completely resistant to thrombotic arterial occlusion (P<0.01). Three weeks after injury, neointimal hyperplasia (from a-smooth muscle actin-positive cells) was significantly less (P<0.001) in arteries from ApoE -/-, HPS3 -/- mice. In ApoE -/-, HPS3 -/- mice, there were also pronounced reductions in arterial inflammation, as indicated by a 74% decrease in CD45-positive leukocytes (P<0.01) and a 73% decrease in Mac-3-positive macrophages (P<0.05). Conclusions-In atherosclerotic mice, reduced platelet dense-granule secretion is associated with marked protection against the development of arterial thrombosis, inflammation, and neointimal hyperplasia after vascular injury,

Original languageEnglish (US)
Pages (from-to)785-791
Number of pages7
JournalCirculation
Volume120
Issue number9
DOIs
StatePublished - Nov 12 2009

Fingerprint

Apolipoproteins E
Thrombosis
Blood Platelets
Arteritis
Hyperplasia
Atherosclerosis
Wounds and Injuries
Leukocytes
Hermanski-Pudlak Syndrome
Vascular Remodeling
P-Selectin
Secretory Pathway
Vascular System Injuries
High Fat Diet
Platelet Count
Vascular Diseases
Platelet Aggregation
Carotid Arteries
Smooth Muscle
Actins

Keywords

  • Arteriosclerosis
  • Atherosclerosis
  • Carotid arteries
  • Platelet-derived factors
  • Platelets
  • Thrombosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Platelet dense-granule secretion plays a critical role in thrombosis and subsequent vascular remodeling in atherosclerotic mice. / King, Sarah M.; McNamee, Rachel A.; Houng, Aiilyan K.; Patel, Rakesh; Brands, Michael; Reed, Guy L.

In: Circulation, Vol. 120, No. 9, 12.11.2009, p. 785-791.

Research output: Contribution to journalArticle

King, Sarah M. ; McNamee, Rachel A. ; Houng, Aiilyan K. ; Patel, Rakesh ; Brands, Michael ; Reed, Guy L. / Platelet dense-granule secretion plays a critical role in thrombosis and subsequent vascular remodeling in atherosclerotic mice. In: Circulation. 2009 ; Vol. 120, No. 9. pp. 785-791.
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abstract = "Background-Platelet aggregation plays a critical role in myocardial infarction and stroke; however, the role of platelet secretion in atherosclerotic vascular disease is poorly understood. Therefore, we examined the hypothesis that platelet dense-granule secretion modulates thrombosis, inflammation, and atherosclerotic vascular remodeling after injury. Methods and Results-Functional deletion of the Hermansky-Pudlak syndrome 3 gene (HPS 3 -/-) markedly reduces platelet dense-granule secretion. HPS3 -/- mice have normal platelet counts, platelet morphology, and a-granule number, as well as maximal secretion of the a-granule marker P-selectin; however, their capacity to form platelet-leukocyte aggregates is significantly reduced (P<0.05). To examine the role of platelet dense-granule secretion in these processes, atherosclerosis-prone mice with combined genetic deficiency of apolipoprotein E and HPS3 (ApoE -/-, HPS3 -/-) were compared with congenie, atherosclerosis-prone mice with normal platelet secretion (ApoE -/-, HPS3 +/+). After 16 to 18 weeks on a high-fat diet, both groups of mice had similar fasting cholesterol levels and body weight. Carotid arteries of ApoE -/-, HPS3 +/+ mice thrombosed rapidly after FeCl 3 injury, but ApoE -/-, HPS3 -/- mice were completely resistant to thrombotic arterial occlusion (P<0.01). Three weeks after injury, neointimal hyperplasia (from a-smooth muscle actin-positive cells) was significantly less (P<0.001) in arteries from ApoE -/-, HPS3 -/- mice. In ApoE -/-, HPS3 -/- mice, there were also pronounced reductions in arterial inflammation, as indicated by a 74{\%} decrease in CD45-positive leukocytes (P<0.01) and a 73{\%} decrease in Mac-3-positive macrophages (P<0.05). Conclusions-In atherosclerotic mice, reduced platelet dense-granule secretion is associated with marked protection against the development of arterial thrombosis, inflammation, and neointimal hyperplasia after vascular injury,",
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AU - King, Sarah M.

AU - McNamee, Rachel A.

AU - Houng, Aiilyan K.

AU - Patel, Rakesh

AU - Brands, Michael

AU - Reed, Guy L.

PY - 2009/11/12

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N2 - Background-Platelet aggregation plays a critical role in myocardial infarction and stroke; however, the role of platelet secretion in atherosclerotic vascular disease is poorly understood. Therefore, we examined the hypothesis that platelet dense-granule secretion modulates thrombosis, inflammation, and atherosclerotic vascular remodeling after injury. Methods and Results-Functional deletion of the Hermansky-Pudlak syndrome 3 gene (HPS 3 -/-) markedly reduces platelet dense-granule secretion. HPS3 -/- mice have normal platelet counts, platelet morphology, and a-granule number, as well as maximal secretion of the a-granule marker P-selectin; however, their capacity to form platelet-leukocyte aggregates is significantly reduced (P<0.05). To examine the role of platelet dense-granule secretion in these processes, atherosclerosis-prone mice with combined genetic deficiency of apolipoprotein E and HPS3 (ApoE -/-, HPS3 -/-) were compared with congenie, atherosclerosis-prone mice with normal platelet secretion (ApoE -/-, HPS3 +/+). After 16 to 18 weeks on a high-fat diet, both groups of mice had similar fasting cholesterol levels and body weight. Carotid arteries of ApoE -/-, HPS3 +/+ mice thrombosed rapidly after FeCl 3 injury, but ApoE -/-, HPS3 -/- mice were completely resistant to thrombotic arterial occlusion (P<0.01). Three weeks after injury, neointimal hyperplasia (from a-smooth muscle actin-positive cells) was significantly less (P<0.001) in arteries from ApoE -/-, HPS3 -/- mice. In ApoE -/-, HPS3 -/- mice, there were also pronounced reductions in arterial inflammation, as indicated by a 74% decrease in CD45-positive leukocytes (P<0.01) and a 73% decrease in Mac-3-positive macrophages (P<0.05). Conclusions-In atherosclerotic mice, reduced platelet dense-granule secretion is associated with marked protection against the development of arterial thrombosis, inflammation, and neointimal hyperplasia after vascular injury,

AB - Background-Platelet aggregation plays a critical role in myocardial infarction and stroke; however, the role of platelet secretion in atherosclerotic vascular disease is poorly understood. Therefore, we examined the hypothesis that platelet dense-granule secretion modulates thrombosis, inflammation, and atherosclerotic vascular remodeling after injury. Methods and Results-Functional deletion of the Hermansky-Pudlak syndrome 3 gene (HPS 3 -/-) markedly reduces platelet dense-granule secretion. HPS3 -/- mice have normal platelet counts, platelet morphology, and a-granule number, as well as maximal secretion of the a-granule marker P-selectin; however, their capacity to form platelet-leukocyte aggregates is significantly reduced (P<0.05). To examine the role of platelet dense-granule secretion in these processes, atherosclerosis-prone mice with combined genetic deficiency of apolipoprotein E and HPS3 (ApoE -/-, HPS3 -/-) were compared with congenie, atherosclerosis-prone mice with normal platelet secretion (ApoE -/-, HPS3 +/+). After 16 to 18 weeks on a high-fat diet, both groups of mice had similar fasting cholesterol levels and body weight. Carotid arteries of ApoE -/-, HPS3 +/+ mice thrombosed rapidly after FeCl 3 injury, but ApoE -/-, HPS3 -/- mice were completely resistant to thrombotic arterial occlusion (P<0.01). Three weeks after injury, neointimal hyperplasia (from a-smooth muscle actin-positive cells) was significantly less (P<0.001) in arteries from ApoE -/-, HPS3 -/- mice. In ApoE -/-, HPS3 -/- mice, there were also pronounced reductions in arterial inflammation, as indicated by a 74% decrease in CD45-positive leukocytes (P<0.01) and a 73% decrease in Mac-3-positive macrophages (P<0.05). Conclusions-In atherosclerotic mice, reduced platelet dense-granule secretion is associated with marked protection against the development of arterial thrombosis, inflammation, and neointimal hyperplasia after vascular injury,

KW - Arteriosclerosis

KW - Atherosclerosis

KW - Carotid arteries

KW - Platelet-derived factors

KW - Platelets

KW - Thrombosis

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