PLD1 negatively regulates dendritic branching

Yan Bing Zhu, Kai Kang, Ying Zhang, Cai Qi, Gang Li, Dongmin Yin, Yun Wang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Neurons have characteristic dendritic arborization patterns that contribute to information processing. One essential component of dendritic arborization is the formation of a specific number of branches. Although intracellular pathways promoting dendritic growth and branching are being elucidated, the mechanisms that negatively regulate the branching of dendrites remain enigmatic. In this study, using gain-of-function and loss-of-function studies, we show that phospholipase D1 (PLD1) acts as a negative regulator of dendritic branching in cultured hippocampal neurons from embryonic day 18 rat embryos. Overexpression of wild-type PLD1 (WT-PLD1) decreases the complexity of dendrites, whereas knockdown or inhibition of PLD1 increases dendritic branching. We further demonstrated that PLD1 acts downstream of RhoA, one of the small Rho GTPases, to suppress dendritic branching. The restriction of dendritic branching by constitutively active RhoA (V14-RhoA) can be partially rescued by knockdown of PLD1. Moreover, the inhibition of dendritic branching by V14-RhoA andWT-PLD1can be partially ameliorated by reducing the level of phosphatidic acid (PA), which is the enzymatic product of PLD1. Together, these results suggest that RhoA-PLD1-PA may represent a novel signaling pathway in the restriction of dendritic branching and may thus provide insight into the mechanisms of dendritic morphogenesis.

Original languageEnglish (US)
Pages (from-to)7960-7969
Number of pages10
JournalJournal of Neuroscience
Volume32
Issue number23
DOIs
StatePublished - Jun 6 2012

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Phosphatidic Acids
Neuronal Plasticity
Dendrites
Neurons
rho GTP-Binding Proteins
Monomeric GTP-Binding Proteins
phospholipase D1
Automatic Data Processing
Morphogenesis
Embryonic Structures
Growth

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Zhu, Y. B., Kang, K., Zhang, Y., Qi, C., Li, G., Yin, D., & Wang, Y. (2012). PLD1 negatively regulates dendritic branching. Journal of Neuroscience, 32(23), 7960-7969. https://doi.org/10.1523/JNEUROSCI.5378-11.2012

PLD1 negatively regulates dendritic branching. / Zhu, Yan Bing; Kang, Kai; Zhang, Ying; Qi, Cai; Li, Gang; Yin, Dongmin; Wang, Yun.

In: Journal of Neuroscience, Vol. 32, No. 23, 06.06.2012, p. 7960-7969.

Research output: Contribution to journalArticle

Zhu, YB, Kang, K, Zhang, Y, Qi, C, Li, G, Yin, D & Wang, Y 2012, 'PLD1 negatively regulates dendritic branching', Journal of Neuroscience, vol. 32, no. 23, pp. 7960-7969. https://doi.org/10.1523/JNEUROSCI.5378-11.2012
Zhu YB, Kang K, Zhang Y, Qi C, Li G, Yin D et al. PLD1 negatively regulates dendritic branching. Journal of Neuroscience. 2012 Jun 6;32(23):7960-7969. https://doi.org/10.1523/JNEUROSCI.5378-11.2012
Zhu, Yan Bing ; Kang, Kai ; Zhang, Ying ; Qi, Cai ; Li, Gang ; Yin, Dongmin ; Wang, Yun. / PLD1 negatively regulates dendritic branching. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 23. pp. 7960-7969.
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