Plutonium-induced Proliferative Lesions and Pulmonary Epithelial Neoplasms in the Rat: Immunohistochemical and Ultrastructural Evidence for Their Origin from Type II Pneumocytes

R. A. Herbert, B. S. Stegelmeier, N. A. Gillett, F. F. Hahn, A. H. Rebar, W. W. Carlton, G. Singh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Immunohistochemistry and transmission electron microscopy were used to clarify the cellular origin for plutonium-239-induced pulmonary proliferative (preneoplastic) epithelial lesions and epithelial neoplasms in F344 rats. Examples of each histologic type of proliferative lesion and neoplasm were stained by the avidin-biotin complex immunoperoxidase method using antibodies to rat surfactant apoprotein and Clara cell antigen. Rat surfactant apoprotein immunostaining was detected in type II pneumocytes in sections of normal lung, in the cells of the proliferative lesions classified histologically as alveolar epithelial hyperplasia (51) and mixed foci (alveolar epithelial hyperplasia with fibrosis) (30), and in adenomas (2), adenocarcinomas (3), and adenosquamous carcinomas (2). With the exception of one adenosquamous carcinoma, Clara cell antigen immunostaining was not detected in any of the pulmonary lesions but was detected in nonciliated cuboidal epithelial (Clara) cells in normal bronchioles. The epithelial cells of the proliferative lesions and neoplasms had ultrastructural features consistent with type II pneumocytes, i.e., the presence of cytoplasmic lamellar and multivesicular bodies. The results of these studies indicate that the majority of plutonium-induced proliferative epithelial lesions and neoplasms in the rat originate from alveolar type II pneumocytes.

Original languageEnglish (US)
Pages (from-to)366-374
Number of pages9
JournalVeterinary Pathology
Volume31
Issue number3
DOIs
StatePublished - May 1994

Fingerprint

pneumocytes
Plutonium
Alveolar Epithelial Cells
plutonium
Glandular and Epithelial Neoplasms
lesions (animal)
Adenosquamous Carcinoma
Lung Neoplasms
Apoproteins
lungs
Surface-Active Agents
Lung
neoplasms
Hyperplasia
rats
Epithelial Cells
Multivesicular Bodies
Bronchioles
apoproteins
Avidin

Keywords

  • Immunohistochemistry
  • lung
  • neoplasia
  • plutonium
  • rats
  • type II pneumocytes
  • ultrastructure

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Plutonium-induced Proliferative Lesions and Pulmonary Epithelial Neoplasms in the Rat : Immunohistochemical and Ultrastructural Evidence for Their Origin from Type II Pneumocytes. / Herbert, R. A.; Stegelmeier, B. S.; Gillett, N. A.; Hahn, F. F.; Rebar, A. H.; Carlton, W. W.; Singh, G.

In: Veterinary Pathology, Vol. 31, No. 3, 05.1994, p. 366-374.

Research output: Contribution to journalArticle

Herbert, R. A. ; Stegelmeier, B. S. ; Gillett, N. A. ; Hahn, F. F. ; Rebar, A. H. ; Carlton, W. W. ; Singh, G. / Plutonium-induced Proliferative Lesions and Pulmonary Epithelial Neoplasms in the Rat : Immunohistochemical and Ultrastructural Evidence for Their Origin from Type II Pneumocytes. In: Veterinary Pathology. 1994 ; Vol. 31, No. 3. pp. 366-374.
@article{a31e18edfc9845f8a33db45bb2acbfe6,
title = "Plutonium-induced Proliferative Lesions and Pulmonary Epithelial Neoplasms in the Rat: Immunohistochemical and Ultrastructural Evidence for Their Origin from Type II Pneumocytes",
abstract = "Immunohistochemistry and transmission electron microscopy were used to clarify the cellular origin for plutonium-239-induced pulmonary proliferative (preneoplastic) epithelial lesions and epithelial neoplasms in F344 rats. Examples of each histologic type of proliferative lesion and neoplasm were stained by the avidin-biotin complex immunoperoxidase method using antibodies to rat surfactant apoprotein and Clara cell antigen. Rat surfactant apoprotein immunostaining was detected in type II pneumocytes in sections of normal lung, in the cells of the proliferative lesions classified histologically as alveolar epithelial hyperplasia (51) and mixed foci (alveolar epithelial hyperplasia with fibrosis) (30), and in adenomas (2), adenocarcinomas (3), and adenosquamous carcinomas (2). With the exception of one adenosquamous carcinoma, Clara cell antigen immunostaining was not detected in any of the pulmonary lesions but was detected in nonciliated cuboidal epithelial (Clara) cells in normal bronchioles. The epithelial cells of the proliferative lesions and neoplasms had ultrastructural features consistent with type II pneumocytes, i.e., the presence of cytoplasmic lamellar and multivesicular bodies. The results of these studies indicate that the majority of plutonium-induced proliferative epithelial lesions and neoplasms in the rat originate from alveolar type II pneumocytes.",
keywords = "Immunohistochemistry, lung, neoplasia, plutonium, rats, type II pneumocytes, ultrastructure",
author = "Herbert, {R. A.} and Stegelmeier, {B. S.} and Gillett, {N. A.} and Hahn, {F. F.} and Rebar, {A. H.} and Carlton, {W. W.} and G. Singh",
year = "1994",
month = "5",
doi = "10.1177/030098589403100310",
language = "English (US)",
volume = "31",
pages = "366--374",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Plutonium-induced Proliferative Lesions and Pulmonary Epithelial Neoplasms in the Rat

T2 - Immunohistochemical and Ultrastructural Evidence for Their Origin from Type II Pneumocytes

AU - Herbert, R. A.

AU - Stegelmeier, B. S.

AU - Gillett, N. A.

AU - Hahn, F. F.

AU - Rebar, A. H.

AU - Carlton, W. W.

AU - Singh, G.

PY - 1994/5

Y1 - 1994/5

N2 - Immunohistochemistry and transmission electron microscopy were used to clarify the cellular origin for plutonium-239-induced pulmonary proliferative (preneoplastic) epithelial lesions and epithelial neoplasms in F344 rats. Examples of each histologic type of proliferative lesion and neoplasm were stained by the avidin-biotin complex immunoperoxidase method using antibodies to rat surfactant apoprotein and Clara cell antigen. Rat surfactant apoprotein immunostaining was detected in type II pneumocytes in sections of normal lung, in the cells of the proliferative lesions classified histologically as alveolar epithelial hyperplasia (51) and mixed foci (alveolar epithelial hyperplasia with fibrosis) (30), and in adenomas (2), adenocarcinomas (3), and adenosquamous carcinomas (2). With the exception of one adenosquamous carcinoma, Clara cell antigen immunostaining was not detected in any of the pulmonary lesions but was detected in nonciliated cuboidal epithelial (Clara) cells in normal bronchioles. The epithelial cells of the proliferative lesions and neoplasms had ultrastructural features consistent with type II pneumocytes, i.e., the presence of cytoplasmic lamellar and multivesicular bodies. The results of these studies indicate that the majority of plutonium-induced proliferative epithelial lesions and neoplasms in the rat originate from alveolar type II pneumocytes.

AB - Immunohistochemistry and transmission electron microscopy were used to clarify the cellular origin for plutonium-239-induced pulmonary proliferative (preneoplastic) epithelial lesions and epithelial neoplasms in F344 rats. Examples of each histologic type of proliferative lesion and neoplasm were stained by the avidin-biotin complex immunoperoxidase method using antibodies to rat surfactant apoprotein and Clara cell antigen. Rat surfactant apoprotein immunostaining was detected in type II pneumocytes in sections of normal lung, in the cells of the proliferative lesions classified histologically as alveolar epithelial hyperplasia (51) and mixed foci (alveolar epithelial hyperplasia with fibrosis) (30), and in adenomas (2), adenocarcinomas (3), and adenosquamous carcinomas (2). With the exception of one adenosquamous carcinoma, Clara cell antigen immunostaining was not detected in any of the pulmonary lesions but was detected in nonciliated cuboidal epithelial (Clara) cells in normal bronchioles. The epithelial cells of the proliferative lesions and neoplasms had ultrastructural features consistent with type II pneumocytes, i.e., the presence of cytoplasmic lamellar and multivesicular bodies. The results of these studies indicate that the majority of plutonium-induced proliferative epithelial lesions and neoplasms in the rat originate from alveolar type II pneumocytes.

KW - Immunohistochemistry

KW - lung

KW - neoplasia

KW - plutonium

KW - rats

KW - type II pneumocytes

KW - ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0028435224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028435224&partnerID=8YFLogxK

U2 - 10.1177/030098589403100310

DO - 10.1177/030098589403100310

M3 - Article

C2 - 8053132

AN - SCOPUS:0028435224

VL - 31

SP - 366

EP - 374

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 3

ER -