Pneumatic pressure bioreactor for cyclic hydrostatic stress application

Mechanobiology effects on periodontal ligament cells

Karl H. Wenger, Ahmed R. El-Awady, Regina L W Messer, Mohamed M.H. Sharawy, Greg White, Carol A. Lapp

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A bioreactor system was developed to provide high-amplitude cyclic hydrostatic compressive stress (cHSC) using compressed air mixed commercially as needed to create partial pressures of oxygen and carbon dioxide appropriate for the cells under investigation. Operating pressures as high as 300 psi are achievable in this system at cyclic speeds of up to 0.2 Hz. In this study, ligamentous fibroblasts from human periodontal ligaments (n = 6) were compressed on two consecutive days at 150 psi for 3 h each day, and the mRNA for families of extracellular matrix protein and protease isoforms was evaluated by real-time PCR array. Several integrins were significantly upregulated, most notably alpha-3 (6.4-fold), as was SPG7 (12.1-fold). Among the collagens, Col8a1 was highly upregulated at 53.5-fold, with Col6a1, Col6a2, and Col7a1 also significantly upregulated 4.4- to 8.5-fold. MMP-1 was the most affected at 122.9-fold upregulation. MMP-14 likewise increased 17.8-fold with slight reductions for the gelatinases and a significant increase of TIMP-2 at 5.8-fold. The development of this bioreactor system and its utility in characterizing periodontal ligament fibroblast mechanobiology in intermediate-term testing hold promise for better simulating the conditions of the musculoskeletal system and the large cyclic compressive stresses joints may experience in gait, exertion, and mastication.

Original languageEnglish (US)
Pages (from-to)1072-1079
Number of pages8
JournalJournal of Applied Physiology
Volume111
Issue number4
DOIs
StatePublished - Oct 1 2011
Externally publishedYes

Fingerprint

Biophysics
Periodontal Ligament
Bioreactors
Matrix Metalloproteinases
Fibroblasts
Compressed Air
Pressure
Tissue Inhibitor of Metalloproteinase-2
Musculoskeletal System
Gelatinases
Partial Pressure
Extracellular Matrix Proteins
Mastication
Gait
Carbon Dioxide
Integrins
Real-Time Polymerase Chain Reaction
Protein Isoforms
Peptide Hydrolases
Up-Regulation

Keywords

  • Cell adhesion
  • Compression
  • Extracellular matrix
  • Fibroblast
  • Gene expression
  • PCR array
  • Protease
  • Real-time PCR

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Pneumatic pressure bioreactor for cyclic hydrostatic stress application : Mechanobiology effects on periodontal ligament cells. / Wenger, Karl H.; El-Awady, Ahmed R.; Messer, Regina L W; Sharawy, Mohamed M.H.; White, Greg; Lapp, Carol A.

In: Journal of Applied Physiology, Vol. 111, No. 4, 01.10.2011, p. 1072-1079.

Research output: Contribution to journalArticle

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