Pneumocystis carinii: Surface reactive carbohydrates detected by lectin probes

Melanie T. Cushion, Jamie A. DeStefano, Peter D. Walzer

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Pneumocystis carinii obtained from rat lungs (RLH) and in vitro culture (RTC) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. Percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. All RLH and RTC derived organisms bound strongly concanavalin A (Con A), and wheat germ agglutinin (WGA). However, differences in soybean agglutinin (SBA) binding between RLH and RTC organisms was observed. Different subsets of the organism bound lectins from Griffonia simplicifolia I, Maclura pomifera, and Bauhinia purpurea, indicating heterogeneity in the surface carbohydrates within each of the RLH and RTC populations. Eight lectins reacted very weakly or not at all: Dolichos bifiorus, Arachis hypogaea, Griffonia simplicifolia I-β4, Solanum tuberosum, Ulex europeus, Griffonia simplicifolia II, Viscum album, and Limax flavus. The results indicate that P. carinii trophic and cyst forms have surface constituents containing mannose, N-acetylglucosamine and N-acetylgalactosamine as the predominant carbohydrates. Molecules resembling sialic acid and β-galactose are absent or inaccessible. The surface glycoconjugates identified in these studies may play a role in the adherent properties of P. carinii.

Original languageEnglish (US)
Pages (from-to)137-147
Number of pages11
JournalExperimental Parasitology
Volume67
Issue number2
DOIs
StatePublished - Dec 1988

Fingerprint

Pneumocystis carinii
Griffonia
Lectins
Carbohydrates
Cysts
Maclura
Bauhinia
Viscum album
Dolichos
Ulex
Acetylgalactosamine
Wheat Germ Agglutinins
Glycoconjugates
Acetylglucosamine
N-Acetylneuraminic Acid
Mannose
Concanavalin A
Solanum tuberosum
Fluorescein
Galactose

Keywords

  • Carbohydrates
  • Lectins
  • Opportunistic pathogen
  • Parasite
  • Pneumocystis carinii
  • Pneumonia

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

Cite this

Pneumocystis carinii : Surface reactive carbohydrates detected by lectin probes. / Cushion, Melanie T.; DeStefano, Jamie A.; Walzer, Peter D.

In: Experimental Parasitology, Vol. 67, No. 2, 12.1988, p. 137-147.

Research output: Contribution to journalArticle

Cushion, Melanie T. ; DeStefano, Jamie A. ; Walzer, Peter D. / Pneumocystis carinii : Surface reactive carbohydrates detected by lectin probes. In: Experimental Parasitology. 1988 ; Vol. 67, No. 2. pp. 137-147.
@article{ade3f506ef7145b888f307fd6280f4c0,
title = "Pneumocystis carinii: Surface reactive carbohydrates detected by lectin probes",
abstract = "Pneumocystis carinii obtained from rat lungs (RLH) and in vitro culture (RTC) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. Percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. All RLH and RTC derived organisms bound strongly concanavalin A (Con A), and wheat germ agglutinin (WGA). However, differences in soybean agglutinin (SBA) binding between RLH and RTC organisms was observed. Different subsets of the organism bound lectins from Griffonia simplicifolia I, Maclura pomifera, and Bauhinia purpurea, indicating heterogeneity in the surface carbohydrates within each of the RLH and RTC populations. Eight lectins reacted very weakly or not at all: Dolichos bifiorus, Arachis hypogaea, Griffonia simplicifolia I-β4, Solanum tuberosum, Ulex europeus, Griffonia simplicifolia II, Viscum album, and Limax flavus. The results indicate that P. carinii trophic and cyst forms have surface constituents containing mannose, N-acetylglucosamine and N-acetylgalactosamine as the predominant carbohydrates. Molecules resembling sialic acid and β-galactose are absent or inaccessible. The surface glycoconjugates identified in these studies may play a role in the adherent properties of P. carinii.",
keywords = "Carbohydrates, Lectins, Opportunistic pathogen, Parasite, Pneumocystis carinii, Pneumonia",
author = "Cushion, {Melanie T.} and DeStefano, {Jamie A.} and Walzer, {Peter D.}",
year = "1988",
month = "12",
doi = "10.1016/0014-4894(88)90061-6",
language = "English (US)",
volume = "67",
pages = "137--147",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Pneumocystis carinii

T2 - Surface reactive carbohydrates detected by lectin probes

AU - Cushion, Melanie T.

AU - DeStefano, Jamie A.

AU - Walzer, Peter D.

PY - 1988/12

Y1 - 1988/12

N2 - Pneumocystis carinii obtained from rat lungs (RLH) and in vitro culture (RTC) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. Percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. All RLH and RTC derived organisms bound strongly concanavalin A (Con A), and wheat germ agglutinin (WGA). However, differences in soybean agglutinin (SBA) binding between RLH and RTC organisms was observed. Different subsets of the organism bound lectins from Griffonia simplicifolia I, Maclura pomifera, and Bauhinia purpurea, indicating heterogeneity in the surface carbohydrates within each of the RLH and RTC populations. Eight lectins reacted very weakly or not at all: Dolichos bifiorus, Arachis hypogaea, Griffonia simplicifolia I-β4, Solanum tuberosum, Ulex europeus, Griffonia simplicifolia II, Viscum album, and Limax flavus. The results indicate that P. carinii trophic and cyst forms have surface constituents containing mannose, N-acetylglucosamine and N-acetylgalactosamine as the predominant carbohydrates. Molecules resembling sialic acid and β-galactose are absent or inaccessible. The surface glycoconjugates identified in these studies may play a role in the adherent properties of P. carinii.

AB - Pneumocystis carinii obtained from rat lungs (RLH) and in vitro culture (RTC) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. Percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. All RLH and RTC derived organisms bound strongly concanavalin A (Con A), and wheat germ agglutinin (WGA). However, differences in soybean agglutinin (SBA) binding between RLH and RTC organisms was observed. Different subsets of the organism bound lectins from Griffonia simplicifolia I, Maclura pomifera, and Bauhinia purpurea, indicating heterogeneity in the surface carbohydrates within each of the RLH and RTC populations. Eight lectins reacted very weakly or not at all: Dolichos bifiorus, Arachis hypogaea, Griffonia simplicifolia I-β4, Solanum tuberosum, Ulex europeus, Griffonia simplicifolia II, Viscum album, and Limax flavus. The results indicate that P. carinii trophic and cyst forms have surface constituents containing mannose, N-acetylglucosamine and N-acetylgalactosamine as the predominant carbohydrates. Molecules resembling sialic acid and β-galactose are absent or inaccessible. The surface glycoconjugates identified in these studies may play a role in the adherent properties of P. carinii.

KW - Carbohydrates

KW - Lectins

KW - Opportunistic pathogen

KW - Parasite

KW - Pneumocystis carinii

KW - Pneumonia

UR - http://www.scopus.com/inward/record.url?scp=0024208371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024208371&partnerID=8YFLogxK

U2 - 10.1016/0014-4894(88)90061-6

DO - 10.1016/0014-4894(88)90061-6

M3 - Article

C2 - 3142787

AN - SCOPUS:0024208371

VL - 67

SP - 137

EP - 147

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 2

ER -