Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle

Xi Luo, Lu Xue, Hao Xu, Qing Yang Zhao, Qian Wang, Yu Shan She, Dun An Zang, Jinhua Shen, Yong Bo Peng, Ping Zhao, Meng Fei Yu, Weiwei Chen, Li Qun Ma, Shu Chen, Shanshan Chen, Xiangning Fu, Sheng Hu, Xiaowei Nie, Chenyou Shen, Chunbin ZouGangjian Qin, Jiapei Dai, Guangju Ji, Yunchao Su, Shen Hu, Jingyu Chen, Qing Hua Liu

Research output: Contribution to journalArticle

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Abstract

Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L. contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K+-induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH). Additionally, the ACH-induced precontraction was partly reduced by pyrazole-3 (Pyr3), a selective blocker of TRPC3 and stromal interaction molecule (STIM)/Orai channels. These channel-mediated currents were inhibited by the compounds present in P. aviculare extracts, suggesting that this inhibition was mediated by LVDCCs, TRPC3 and/or STIM/Orai channels. Moreover, these channel-mediated currents were inhibited by quercetin, which is present in P. aviculare extracts. Furthermore, quercetin inhibited ACH-induced precontraction in ASM. Overall, our data indicate that the ethyl acetate fraction of P. aviculare and quercetin can inhibit Ca2+-permeant LVDCCs, TRPC3 and STIM/Orai channels, which inhibits the precontraction of ASM. These findings suggest that P. aviculare could be used to develop new bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease.

Original languageEnglish (US)
Article number3114
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Polygonum
Quercetin
Smooth Muscle
Acetylcholine
Bronchodilator Agents
Nifedipine
Obstructive Lung Diseases
Chronic Obstructive Pulmonary Disease
Asthma
Stromal Interaction Molecules

ASJC Scopus subject areas

  • General

Cite this

Luo, X., Xue, L., Xu, H., Zhao, Q. Y., Wang, Q., She, Y. S., ... Liu, Q. H. (2018). Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle. Scientific Reports, 8(1), [3114]. https://doi.org/10.1038/s41598-018-20409-x

Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle. / Luo, Xi; Xue, Lu; Xu, Hao; Zhao, Qing Yang; Wang, Qian; She, Yu Shan; Zang, Dun An; Shen, Jinhua; Peng, Yong Bo; Zhao, Ping; Yu, Meng Fei; Chen, Weiwei; Ma, Li Qun; Chen, Shu; Chen, Shanshan; Fu, Xiangning; Hu, Sheng; Nie, Xiaowei; Shen, Chenyou; Zou, Chunbin; Qin, Gangjian; Dai, Jiapei; Ji, Guangju; Su, Yunchao; Hu, Shen; Chen, Jingyu; Liu, Qing Hua.

In: Scientific Reports, Vol. 8, No. 1, 3114, 01.12.2018.

Research output: Contribution to journalArticle

Luo, X, Xue, L, Xu, H, Zhao, QY, Wang, Q, She, YS, Zang, DA, Shen, J, Peng, YB, Zhao, P, Yu, MF, Chen, W, Ma, LQ, Chen, S, Chen, S, Fu, X, Hu, S, Nie, X, Shen, C, Zou, C, Qin, G, Dai, J, Ji, G, Su, Y, Hu, S, Chen, J & Liu, QH 2018, 'Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle', Scientific Reports, vol. 8, no. 1, 3114. https://doi.org/10.1038/s41598-018-20409-x
Luo, Xi ; Xue, Lu ; Xu, Hao ; Zhao, Qing Yang ; Wang, Qian ; She, Yu Shan ; Zang, Dun An ; Shen, Jinhua ; Peng, Yong Bo ; Zhao, Ping ; Yu, Meng Fei ; Chen, Weiwei ; Ma, Li Qun ; Chen, Shu ; Chen, Shanshan ; Fu, Xiangning ; Hu, Sheng ; Nie, Xiaowei ; Shen, Chenyou ; Zou, Chunbin ; Qin, Gangjian ; Dai, Jiapei ; Ji, Guangju ; Su, Yunchao ; Hu, Shen ; Chen, Jingyu ; Liu, Qing Hua. / Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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abstract = "Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L. contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K+-induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH). Additionally, the ACH-induced precontraction was partly reduced by pyrazole-3 (Pyr3), a selective blocker of TRPC3 and stromal interaction molecule (STIM)/Orai channels. These channel-mediated currents were inhibited by the compounds present in P. aviculare extracts, suggesting that this inhibition was mediated by LVDCCs, TRPC3 and/or STIM/Orai channels. Moreover, these channel-mediated currents were inhibited by quercetin, which is present in P. aviculare extracts. Furthermore, quercetin inhibited ACH-induced precontraction in ASM. Overall, our data indicate that the ethyl acetate fraction of P. aviculare and quercetin can inhibit Ca2+-permeant LVDCCs, TRPC3 and STIM/Orai channels, which inhibits the precontraction of ASM. These findings suggest that P. aviculare could be used to develop new bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease.",
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AU - Luo, Xi

AU - Xue, Lu

AU - Xu, Hao

AU - Zhao, Qing Yang

AU - Wang, Qian

AU - She, Yu Shan

AU - Zang, Dun An

AU - Shen, Jinhua

AU - Peng, Yong Bo

AU - Zhao, Ping

AU - Yu, Meng Fei

AU - Chen, Weiwei

AU - Ma, Li Qun

AU - Chen, Shu

AU - Chen, Shanshan

AU - Fu, Xiangning

AU - Hu, Sheng

AU - Nie, Xiaowei

AU - Shen, Chenyou

AU - Zou, Chunbin

AU - Qin, Gangjian

AU - Dai, Jiapei

AU - Ji, Guangju

AU - Su, Yunchao

AU - Hu, Shen

AU - Chen, Jingyu

AU - Liu, Qing Hua

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L. contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K+-induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH). Additionally, the ACH-induced precontraction was partly reduced by pyrazole-3 (Pyr3), a selective blocker of TRPC3 and stromal interaction molecule (STIM)/Orai channels. These channel-mediated currents were inhibited by the compounds present in P. aviculare extracts, suggesting that this inhibition was mediated by LVDCCs, TRPC3 and/or STIM/Orai channels. Moreover, these channel-mediated currents were inhibited by quercetin, which is present in P. aviculare extracts. Furthermore, quercetin inhibited ACH-induced precontraction in ASM. Overall, our data indicate that the ethyl acetate fraction of P. aviculare and quercetin can inhibit Ca2+-permeant LVDCCs, TRPC3 and STIM/Orai channels, which inhibits the precontraction of ASM. These findings suggest that P. aviculare could be used to develop new bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease.

AB - Because of the serious side effects of the currently used bronchodilators, new compounds with similar functions must be developed. We screened several herbs and found that Polygonum aviculare L. contains ingredients that inhibit the precontraction of mouse and human airway smooth muscle (ASM). High K+-induced precontraction in ASM was completely inhibited by nifedipine, a selective blocker of L-type voltage-dependent Ca2+ channels (LVDCCs). However, nifedipine only partially reduced the precontraction induced by acetylcholine chloride (ACH). Additionally, the ACH-induced precontraction was partly reduced by pyrazole-3 (Pyr3), a selective blocker of TRPC3 and stromal interaction molecule (STIM)/Orai channels. These channel-mediated currents were inhibited by the compounds present in P. aviculare extracts, suggesting that this inhibition was mediated by LVDCCs, TRPC3 and/or STIM/Orai channels. Moreover, these channel-mediated currents were inhibited by quercetin, which is present in P. aviculare extracts. Furthermore, quercetin inhibited ACH-induced precontraction in ASM. Overall, our data indicate that the ethyl acetate fraction of P. aviculare and quercetin can inhibit Ca2+-permeant LVDCCs, TRPC3 and STIM/Orai channels, which inhibits the precontraction of ASM. These findings suggest that P. aviculare could be used to develop new bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease.

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