TY - JOUR
T1 - Porphyromonas gingivalis lipopolysaccharide affinity for two casting alloys
AU - Knoernschild, Kent L.
AU - Tompkins, Geoffrey R.
AU - Lefebvre, Carol A.
AU - Schuster, George S.
N1 - Funding Information:
In the shift from periodontal health to gingivitis, the proportion of gram-negative bacteria in the crevicular microbial flora increases. I-5 Among this gram-negative population, Porphyromonas gingivalis may be found. 6 P. gingi-valis has been recovered in low numbers from patients with a healthy periodontium or gingivitis/However, P. gingi-valis prevalence increases with periodontitis and maintains elevated levels after recovery. S Thus, P. gingivalis is a frequent inhabitant of both normal and diseased gingival sulci. P. gingivalis manufactures endotoxin, a cell envelope lipopolysaccharide (LPS) with a wide spectrum of molecular weights, which is released after cell death and lysis. This LPS is capable of inhibiting human cell growth. 9 In addition, LPS can stimulate osteoclasis 1~ and a variety of pathways involved with inflammation. 14-17 Therefore, LPS may contribute to insidious periodontal deterioration by delaying healing and encouraging tissue degradation. The presence of LPSin the crevicular fluid 1S, 19 and the adherence of LPS to subgingival cementum 2~ has been documented. In vitro studies also have substantiated Escherichia coli LPS adherence to commonly used dental casting alloys 21 and E. coli and P. gingivalis LPS adherence to resins. 22 Because LPS is capable of crossing crevicular Supported by the American Fund for Dental Health, the Medical College of Georgia Research Institute, Inc. and the Medical College of Georgia Dental Foundation. aAssistant Professor, Department of Oral Rehabilitation and De-partment of Oral Biology. bAssistant Professor, Department of Oral Biology. CAssociate Professor, Department of Oral Biology. dIone and Arthur Merritt Professor, Department of Oral Rehabil-itation and Department of Oral Biology. Copyright 9 1995 by The Editorial Council of THE JOURNALO F PROSTHETICD ENTISTRY. 0022-3913/95/$3.00 + 0. 10/1/64121 epithelial barriers, 23 LPS adherence to tooth structure or restorative materials could support continuous perfusion of the biologically active toxin into surrounding periodontal tissues. This perfusion may encourage soft tissue inflammation.
PY - 1995/7
Y1 - 1995/7
N2 - With the exception of plaque, the affinity of biologically active bacterial products for restorative materials and the influence of that affinity on periodontal health has not been detailed. This study recognized that Porphyromonas gingivalis endotoxin, which is cell envelope lipopolysaccharide (LPS) produced by a bacterium that is common to the crevicular microbial flora, has an affinity for dental casting alloys. Regardless of surface finish, no difference in LPS initial adherence or eluation was recorded between a type III gold or nickel-chromium-beryllium alloy (p>0.05), but LPS readily adhered and remained attached to both alloys. LPS affinity could contribute to periodontal inflammation in tissues that approximate restorations fabricated from either alloy.
AB - With the exception of plaque, the affinity of biologically active bacterial products for restorative materials and the influence of that affinity on periodontal health has not been detailed. This study recognized that Porphyromonas gingivalis endotoxin, which is cell envelope lipopolysaccharide (LPS) produced by a bacterium that is common to the crevicular microbial flora, has an affinity for dental casting alloys. Regardless of surface finish, no difference in LPS initial adherence or eluation was recorded between a type III gold or nickel-chromium-beryllium alloy (p>0.05), but LPS readily adhered and remained attached to both alloys. LPS affinity could contribute to periodontal inflammation in tissues that approximate restorations fabricated from either alloy.
UR - http://www.scopus.com/inward/record.url?scp=0029340732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029340732&partnerID=8YFLogxK
U2 - 10.1016/S0022-3913(05)80225-2
DO - 10.1016/S0022-3913(05)80225-2
M3 - Article
C2 - 7674187
AN - SCOPUS:0029340732
SN - 0022-3913
VL - 74
SP - 33
EP - 38
JO - The Journal of Prosthetic Dentistry
JF - The Journal of Prosthetic Dentistry
IS - 1
ER -