Post-translational modification of p53 by ubiquitin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Post-translational modification of p53 by ubiquitin resides in the center of a fine-tuned regulatory network that activates the tumor suppressor in response to genotoxic stress. Inhibition of p53 ubiquitination by DNA damage not only prevents p53 from degradation but also promotes its nuclear accumulation leading to transactivation of a number of downstream genes that are essential for the control of cell cycle progression, cell survival, and cellular senescence. Therefore, there are growing interests in studying p53 ubiquitination under physiological/pathological conditions. We describe herein a cell-based method and an in vitro reconstituted assay that are convenient, reproducible, and adaptable for various experimental conditions for detection of p53 ubiquitination. Wide application of these methods in studying mechanisms underlying regulation of p53 ubiquitination shall assist us in better understanding of the function of the tumor suppressor.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages305-315
Number of pages11
Volume647
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume647
ISSN (Print)10643745

Keywords

  • MDM2
  • p53
  • Post-translational modification
  • Reconstituted assay system
  • Transfection
  • Ubiquitin
  • Ubiquitination

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Yan, C. (2010). Post-translational modification of p53 by ubiquitin. In Methods in Molecular Biology (Vol. 647, pp. 305-315). (Methods in Molecular Biology; Vol. 647). https://doi.org/10.1007/978-1-60761-738-9-19