TY - CHAP
T1 - Post-translational modification of p53 by ubiquitin
AU - Yan, Chunhong
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Post-translational modification of p53 by ubiquitin resides in the center of a fine-tuned regulatory network that activates the tumor suppressor in response to genotoxic stress. Inhibition of p53 ubiquitination by DNA damage not only prevents p53 from degradation but also promotes its nuclear accumulation leading to transactivation of a number of downstream genes that are essential for the control of cell cycle progression, cell survival, and cellular senescence. Therefore, there are growing interests in studying p53 ubiquitination under physiological/pathological conditions. We describe herein a cell-based method and an in vitro reconstituted assay that are convenient, reproducible, and adaptable for various experimental conditions for detection of p53 ubiquitination. Wide application of these methods in studying mechanisms underlying regulation of p53 ubiquitination shall assist us in better understanding of the function of the tumor suppressor.
AB - Post-translational modification of p53 by ubiquitin resides in the center of a fine-tuned regulatory network that activates the tumor suppressor in response to genotoxic stress. Inhibition of p53 ubiquitination by DNA damage not only prevents p53 from degradation but also promotes its nuclear accumulation leading to transactivation of a number of downstream genes that are essential for the control of cell cycle progression, cell survival, and cellular senescence. Therefore, there are growing interests in studying p53 ubiquitination under physiological/pathological conditions. We describe herein a cell-based method and an in vitro reconstituted assay that are convenient, reproducible, and adaptable for various experimental conditions for detection of p53 ubiquitination. Wide application of these methods in studying mechanisms underlying regulation of p53 ubiquitination shall assist us in better understanding of the function of the tumor suppressor.
KW - MDM2
KW - p53
KW - Post-translational modification
KW - Reconstituted assay system
KW - Transfection
KW - Ubiquitin
KW - Ubiquitination
UR - http://www.scopus.com/inward/record.url?scp=79952113112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952113112&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-738-9-19
DO - 10.1007/978-1-60761-738-9-19
M3 - Chapter
C2 - 20694676
SN - 9781607617372
VL - 647
T3 - Methods in Molecular Biology
SP - 305
EP - 315
BT - Methods in Molecular Biology
ER -