Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

Djanybek M. Adyshev, Irina A. Kolosova, Alexander Dmitriyevich Verin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalMolecular Biology Reports
Volume33
Issue number2
DOIs
StatePublished - Jun 1 2006

Fingerprint

Screening
U5 Small Nuclear Ribonucleoproteins
Proteins
Peptide Elongation Factor 2
Actin Cytoskeleton
Cell Adhesion
Glycine
Cell adhesion
Endothelial cells
Hybrid systems
Endothelial Cells
Organizations
Actins
Lung
human PPP1R16B protein

Keywords

  • BacterioMatch two-hybrid system
  • Cytoskeleton
  • Partner proteins
  • TIMAP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening. / Adyshev, Djanybek M.; Kolosova, Irina A.; Verin, Alexander Dmitriyevich.

In: Molecular Biology Reports, Vol. 33, No. 2, 01.06.2006, p. 83-89.

Research output: Contribution to journalArticle

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