Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

Djanybek M. Adyshev; Irina A. Kolosova; Alexander D. Verin

doi:10.1007/s11033-005-2311-y

Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

Djanybek M. Adyshev, Irina A. Kolosova, Alexander D. Verin

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.

Original language	English (US)
Pages (from-to)	83-89
Number of pages	7
Journal	Molecular Biology Reports
Volume	33
Issue number	2
DOIs	https://doi.org/10.1007/s11033-005-2311-y
State	Published - Jun 2006
Externally published	Yes

Keywords

BacterioMatch two-hybrid system
Cytoskeleton
Partner proteins
TIMAP

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/s11033-005-2311-y

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title = "Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening",

abstract = "BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.",

keywords = "BacterioMatch two-hybrid system, Cytoskeleton, Partner proteins, TIMAP",

author = "Adyshev, {Djanybek M.} and Kolosova, {Irina A.} and Verin, {Alexander D.}",

note = "Funding Information: This work was supported by NIH Grants HL 58064, HL 67307, HL 68062, HL 80675 (for AV) and ALA Research Grant for Maryland (for IK). The authors wish to thank Aigerim Adysheva for the technical assistance in preparation of the manuscript.",

year = "2006",

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doi = "10.1007/s11033-005-2311-y",

language = "English (US)",

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T1 - Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

AU - Adyshev, Djanybek M.

AU - Kolosova, Irina A.

AU - Verin, Alexander D.

N1 - Funding Information: This work was supported by NIH Grants HL 58064, HL 67307, HL 68062, HL 80675 (for AV) and ALA Research Grant for Maryland (for IK). The authors wish to thank Aigerim Adysheva for the technical assistance in preparation of the manuscript.

PY - 2006/6

Y1 - 2006/6

N2 - BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.

AB - BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.

KW - BacterioMatch two-hybrid system

KW - Cytoskeleton

KW - Partner proteins

KW - TIMAP

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Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

Abstract

Keywords

ASJC Scopus subject areas

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