Potential protein partners for the human TIMAP revealed by bacterial two-hybrid screening

Djanybek M. Adyshev, Irina A. Kolosova, Alexander D. Verin

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

BacterioMatch Two-Hybrid System (Stratagene) was applied in order to identify potential human TIMAP interaction proteins in the lung. TIMAP highly expressed in endothelial cells and may be involved in endothelial cytoskeletal and barrier regulation. Seven TIMAP interacting partner proteins were identified. Four of identified proteins: cystein and glycine-rich protein 1, eukaryotic translation elongation factor 2, U5 snRNP-specific protein 116 kD, and solute carrier family 3 member 2 are involved in actin cytoskeleton organization, cell adhesion or translation and transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalMolecular Biology Reports
Volume33
Issue number2
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

Keywords

  • BacterioMatch two-hybrid system
  • Cytoskeleton
  • Partner proteins
  • TIMAP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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