Pre-clinical orthotopic murine model of human prostate cancer

Varahram Shahryari, Hannah Nip, Sharanjot Saini, Altaf A. Dar, Soichiro Yamamura, Yozo Mitsui, Melissa Colden, Nathan Bucay, Laura Z. Tabatabai, Kirsten Greene, Guoren Deng, Yuichiro Tanaka, Rajvir Dahiya, Shahana Majid

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To study the multifaceted biology of prostate cancer, pre-clinical in vivo models offer a range of options to uncover critical biological information about this disease. The human orthotopic prostate cancer xenograft mouse model provides a useful alternative approach for understanding the specific interactions between genetically and molecularly altered tumor cells, their organ microenvironment, and for evaluation of efficacy of therapeutic regimens. This is a well characterized model designed to study the molecular events of primary tumor development and it recapitulates the early events in the metastatic cascade prior to embolism and entry of tumor cells into the circulation. Thus it allows elucidation of molecular mechanisms underlying the initial phase of metastatic disease. In addition, this model can annotate drug targets of clinical relevance and is a valuable tool to study prostate cancer progression. In this manuscript we describe a detailed procedure to establish a human orthotopic prostate cancer xenograft mouse model.

Original languageEnglish (US)
Article numbere54125
JournalJournal of Visualized Experiments
Volume2016
Issue number114
DOIs
StatePublished - Aug 29 2016
Externally publishedYes

Keywords

  • Bioimaging
  • Cancer
  • Human orthotopic prostate cancer xenograft mouse model
  • Intraprostatic implantation
  • Issue 114
  • Medicine
  • Mouse model
  • Prostate

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Shahryari, V., Nip, H., Saini, S., Dar, A. A., Yamamura, S., Mitsui, Y., Colden, M., Bucay, N., Tabatabai, L. Z., Greene, K., Deng, G., Tanaka, Y., Dahiya, R., & Majid, S. (2016). Pre-clinical orthotopic murine model of human prostate cancer. Journal of Visualized Experiments, 2016(114), [e54125]. https://doi.org/10.3791/54125