Preferential interaction of the core histone tail domains with linker DNA

Dimitar Angelov, Joseph M Vitolo, Vesco Mutskov, Stefan Dimitrov, Jeffrey J. Hayes

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Within chromatin, the core histone tail domains play critical roles in regulating the structure and accessibility of nucleosomal DNA within the chromatin fiber. Thus, many nuclear processes are facilitated by concomitant posttranslational modification of these domains. However, elucidation of the mechanisms by which the tails mediate such processes awaits definition of tail interactions within chromatin. In this study we have investigated the primary DNA target of the majority of the tails in mononucleosomes. The results clearly show that the tails bind preferentially to "linker" DNA, outside of the DNA encompassed by the nucleosome core. These results have important implications for models of tail function within the chromatin fiber and for in vitro structural and functional studies using nucleosome core particles.

Original languageEnglish (US)
Pages (from-to)6599-6604
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number12
DOIs
StatePublished - Jun 5 2001
Externally publishedYes

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Histones
Chromatin
Nucleosomes
DNA
Post Translational Protein Processing

Keywords

  • Chromatin
  • Nucleosomes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Preferential interaction of the core histone tail domains with linker DNA. / Angelov, Dimitar; Vitolo, Joseph M; Mutskov, Vesco; Dimitrov, Stefan; Hayes, Jeffrey J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 12, 05.06.2001, p. 6599-6604.

Research output: Contribution to journalArticle

Angelov, Dimitar ; Vitolo, Joseph M ; Mutskov, Vesco ; Dimitrov, Stefan ; Hayes, Jeffrey J. / Preferential interaction of the core histone tail domains with linker DNA. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 12. pp. 6599-6604.
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