Prenatal alcohol exposure triggers ceramide-induced apoptosis in neural crest-derived tissues concurrent with defective cranial development

Guanghu Wang, Erhard Bieberich

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Fetal alcohol syndrome (FAS) is caused by maternal alcohol consumption during pregnancy. The reason why specific embryonic tissues are sensitive toward ethanol is not understood. We found that in neural crest-derived cell (NCC) cultures from the first branchial arch of E10 mouse embryos, incubation with ethanol increases the number of apoptotic cells by fivefold. Apoptotic cells stain intensely for ceramide, suggesting that ceramide-induced apoptosis mediates ethanol damage to NCCs. Apoptosis is reduced by incubation with CDP-choline (citicoline), a precursor for the conversion of ceramide to sphingomyelin. Consistent with NCC cultures, ethanol intubation of pregnant mice results in ceramide elevation and increased apoptosis of NCCs in vivo. Ethanol also increases the protein level of prostate apoptosis response 4 (PAR-4), a sensitizer to ceramide-induced apoptosis. Prenatal ethanol exposure is concurrent with malformation of parietal bones in 20% of embryos at day E18. Meninges, a tissue complex derived from NCCs, is disrupted and generates reduced levels of TGF-β1, a growth factor critical for bone and brain development. Ethanol-induced apoptosis of NCCs leading to defects in the meninges may explain the simultaneous presence of cranial bone malformation and cognitive retardation in FAS. In addition, our data suggest that treatment with CDP-choline may alleviate the tissue damage caused by alcohol.

Original languageEnglish (US)
Article numbere46
JournalCell Death and Disease
Volume1
Issue number5
DOIs
StatePublished - May 1 2010

Fingerprint

Neural Crest
Ceramides
Ethanol
Alcohols
Apoptosis
Cytidine Diphosphate Choline
Fetal Alcohol Spectrum Disorders
Meninges
Embryonic Structures
Cell Culture Techniques
Parietal Bone
Branchial Region
Sphingomyelins
Bone Development
Intubation
Alcohol Drinking
Intercellular Signaling Peptides and Proteins
Coloring Agents
Cell Count
Mothers

Keywords

  • Apoptosis
  • Ceramide
  • Ethanol
  • Fetal alcohol syndrome
  • Neural crest

ASJC Scopus subject areas

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

Prenatal alcohol exposure triggers ceramide-induced apoptosis in neural crest-derived tissues concurrent with defective cranial development. / Wang, Guanghu; Bieberich, Erhard.

In: Cell Death and Disease, Vol. 1, No. 5, e46, 01.05.2010.

Research output: Contribution to journalArticle

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