Prenatal diagnosis of sickle cell anemia using PCR and restriction enzyme Dde I.

A. Gürgey, S. Beksaç, L. Mesci, N. Cakar, U. Karakaş, Abdullah Kutlar, C. Altay

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Prenatal diagnosis of sickle cell anemia was carried out in four fetuses using DNA technology. Fetal chorionic villus specimen were obtained at the 10th week of pregnancy from women at risk of giving birth to children with sickle cell anemia. Whole cellular DNA was obtained and the part of the DNA presumed to have a mutation increased after PCR was performed. After the application of Dde I restriction enzyme, mini gel electrophoresis was performed. The study of the electrophoretic patterns of the DNA indicated that one of the four fetuses was unaffected, one was a carrier and the remaining two were affected.

Original languageEnglish (US)
Pages (from-to)159-162
Number of pages4
JournalThe Turkish journal of pediatrics
Volume35
Issue number3
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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    Gürgey, A., Beksaç, S., Mesci, L., Cakar, N., Karakaş, U., Kutlar, A., & Altay, C. (1993). Prenatal diagnosis of sickle cell anemia using PCR and restriction enzyme Dde I. The Turkish journal of pediatrics, 35(3), 159-162.