Abstract
Abstract: In some patients with demyelinating neuropathy there are immunoglobulin M paraproteins that react with carbohydrate determinants shared by myelin‐associated glycoprotein (MAG) and two peripheral nerve acidic glycolipids, termed sulfoglucuronosylglycosphingolipids (SGGLs). To study the antigenicity of these glycolipids, we immunized three New Zealand white rabbits with sulfoglucuronosylparagloboside (SGPG), a major SGGL in peripheral nerve, emulsified in Freund's complete adjuvant and keyhole limpet hemocyanin. All three rabbits inoculated with SGPG showed weight loss and mild weakness, predominantly in their hind feet, 2–5 weeks postinoculation (PI). Two of the three rabbits again showed moderate weakness 3 and 8 months PI, respectively. Electrophysiological studies demonstrated a slowed nerve conduction velocity in the sciatic nerve. Anti‐SGPG antibody titers in sera were detected at dilutions of 1:1,000 to 1:2,500 by an enzyme‐linked immunosorbent assay. Although all three rabbit sera reacted with SGGLs, two reacted with a desulfated form of SGPG and the other did not, suggesting a fine heterogeneity in antigenic specificity. As with sera from patients with demyelinative paraproteinemia, all rabbit sera reacted with MAG in human CNS and PNS myelin. They also reacted with MAG from bovine CNS myelin as well as several low‐molecular‐weight glycoproteins in bovine peripheral nerve myelin. Thus, we demonstrated that the rabbit antisera generated against SGPG have the same or similar antigenic specificity as those of the anti‐MAG M‐proteins from patients with neuropathy. The results suggest that an autoimmune response against the sulfoglucuronosyl residue may participate in the immunopathogenesis of this type of neuropathy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 869-877 |
| Number of pages | 9 |
| Journal | Journal of Neurochemistry |
| Volume | 51 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1988 |
| Externally published | Yes |
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Keywords
- Anti‐myelin‐associated glycoprotein antibody
- Sulfoglucuronosylglycosphingolipids
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
Preparation and Characterization of Antibodies Against a Sulfated Glucuronic Acid‐Containing Glycosphingolipid. / Kohriyama, Tatsuo; Ariga, Toshio; Yu, Robert K.
In: Journal of Neurochemistry, Vol. 51, No. 3, 09.1988, p. 869-877.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation and Characterization of Antibodies Against a Sulfated Glucuronic Acid‐Containing Glycosphingolipid
AU - Kohriyama, Tatsuo
AU - Ariga, Toshio
AU - Yu, Robert K.
PY - 1988/9
Y1 - 1988/9
N2 - Abstract: In some patients with demyelinating neuropathy there are immunoglobulin M paraproteins that react with carbohydrate determinants shared by myelin‐associated glycoprotein (MAG) and two peripheral nerve acidic glycolipids, termed sulfoglucuronosylglycosphingolipids (SGGLs). To study the antigenicity of these glycolipids, we immunized three New Zealand white rabbits with sulfoglucuronosylparagloboside (SGPG), a major SGGL in peripheral nerve, emulsified in Freund's complete adjuvant and keyhole limpet hemocyanin. All three rabbits inoculated with SGPG showed weight loss and mild weakness, predominantly in their hind feet, 2–5 weeks postinoculation (PI). Two of the three rabbits again showed moderate weakness 3 and 8 months PI, respectively. Electrophysiological studies demonstrated a slowed nerve conduction velocity in the sciatic nerve. Anti‐SGPG antibody titers in sera were detected at dilutions of 1:1,000 to 1:2,500 by an enzyme‐linked immunosorbent assay. Although all three rabbit sera reacted with SGGLs, two reacted with a desulfated form of SGPG and the other did not, suggesting a fine heterogeneity in antigenic specificity. As with sera from patients with demyelinative paraproteinemia, all rabbit sera reacted with MAG in human CNS and PNS myelin. They also reacted with MAG from bovine CNS myelin as well as several low‐molecular‐weight glycoproteins in bovine peripheral nerve myelin. Thus, we demonstrated that the rabbit antisera generated against SGPG have the same or similar antigenic specificity as those of the anti‐MAG M‐proteins from patients with neuropathy. The results suggest that an autoimmune response against the sulfoglucuronosyl residue may participate in the immunopathogenesis of this type of neuropathy.
AB - Abstract: In some patients with demyelinating neuropathy there are immunoglobulin M paraproteins that react with carbohydrate determinants shared by myelin‐associated glycoprotein (MAG) and two peripheral nerve acidic glycolipids, termed sulfoglucuronosylglycosphingolipids (SGGLs). To study the antigenicity of these glycolipids, we immunized three New Zealand white rabbits with sulfoglucuronosylparagloboside (SGPG), a major SGGL in peripheral nerve, emulsified in Freund's complete adjuvant and keyhole limpet hemocyanin. All three rabbits inoculated with SGPG showed weight loss and mild weakness, predominantly in their hind feet, 2–5 weeks postinoculation (PI). Two of the three rabbits again showed moderate weakness 3 and 8 months PI, respectively. Electrophysiological studies demonstrated a slowed nerve conduction velocity in the sciatic nerve. Anti‐SGPG antibody titers in sera were detected at dilutions of 1:1,000 to 1:2,500 by an enzyme‐linked immunosorbent assay. Although all three rabbit sera reacted with SGGLs, two reacted with a desulfated form of SGPG and the other did not, suggesting a fine heterogeneity in antigenic specificity. As with sera from patients with demyelinative paraproteinemia, all rabbit sera reacted with MAG in human CNS and PNS myelin. They also reacted with MAG from bovine CNS myelin as well as several low‐molecular‐weight glycoproteins in bovine peripheral nerve myelin. Thus, we demonstrated that the rabbit antisera generated against SGPG have the same or similar antigenic specificity as those of the anti‐MAG M‐proteins from patients with neuropathy. The results suggest that an autoimmune response against the sulfoglucuronosyl residue may participate in the immunopathogenesis of this type of neuropathy.
KW - Anti‐myelin‐associated glycoprotein antibody
KW - Sulfoglucuronosylglycosphingolipids
UR - http://www.scopus.com/inward/record.url?scp=0023772540&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023772540&partnerID=8YFLogxK
U2 - 10.1111/j.1471-4159.1988.tb01823.x
DO - 10.1111/j.1471-4159.1988.tb01823.x
M3 - Article
C2 - 2457655
AN - SCOPUS:0023772540
VL - 51
SP - 869
EP - 877
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 3
ER -