Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum

Noriyuki Kasai, Masaharu Naiki, Toshio Ariga, Yoshio Hirabayashi, Robert K. Yu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Antibodies to N-glycolyl neuraminic acid-containing GM2 ganglioside, GM2(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassy (RIA). One(C-4) of two antisera produced did not cross-react with GM3(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM2(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM2(NeuGc) in human and animal tissues under normal and/or disease condition.

Original languageEnglish (US)
Pages (from-to)334-341
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume129
Issue number2
DOIs
StatePublished - Jun 14 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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