Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum

Noriyuki Kasai, Masaharu Naiki, Toshio Ariga, Yoshio Hirabayashi, Robert K Yu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Antibodies to N-glycolyl neuraminic acid-containing GM2 ganglioside, GM2(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassy (RIA). One(C-4) of two antisera produced did not cross-react with GM3(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM2(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM2(NeuGc) in human and animal tissues under normal and/or disease condition.

Original languageEnglish (US)
Pages (from-to)334-341
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume129
Issue number2
DOIs
StatePublished - Jun 14 1985
Externally publishedYes

Fingerprint

Gangliosides
Immune Sera
Immunodiffusion
Antibodies
G(M2) Ganglioside
Neuraminic Acids
Antibody Specificity
Glycolipids
Chickens
Assays
Animals
Immunoglobulin G
Tissue
GM2 N-glycolylneuraminic acid ganglioside
Serum

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum. / Kasai, Noriyuki; Naiki, Masaharu; Ariga, Toshio; Hirabayashi, Yoshio; Yu, Robert K.

In: Biochemical and Biophysical Research Communications, Vol. 129, No. 2, 14.06.1985, p. 334-341.

Research output: Contribution to journalArticle

Kasai, Noriyuki ; Naiki, Masaharu ; Ariga, Toshio ; Hirabayashi, Yoshio ; Yu, Robert K. / Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum. In: Biochemical and Biophysical Research Communications. 1985 ; Vol. 129, No. 2. pp. 334-341.
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