Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum

Noriyuki Kasai; Masaharu Naiki; Toshio Ariga; Yoshio Hirabayashi; Robert K. Yu

doi:10.1016/0006-291X(85)90156-1

Preparation and specificity of avian anti-GM₂(NeuGc) ganglioside antiserum

Noriyuki Kasai, Masaharu Naiki, Toshio Ariga, Yoshio Hirabayashi, Robert K. Yu

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Antibodies to N-glycolyl neuraminic acid-containing GM₂ ganglioside, GM₂(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassy (RIA). One(C-4) of two antisera produced did not cross-react with GM₃(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM₂(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM₂(NeuGc) in human and animal tissues under normal and/or disease condition.

Original language	English (US)
Pages (from-to)	334-341
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(85)90156-1
State	Published - Jun 14 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0006-291X(85)90156-1

Cite this

@article{f472c8bdc4fe48ed9cb2451f68e136bc,

title = "Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum",

abstract = "Antibodies to N-glycolyl neuraminic acid-containing GM2 ganglioside, GM2(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassy (RIA). One(C-4) of two antisera produced did not cross-react with GM3(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM2(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM2(NeuGc) in human and animal tissues under normal and/or disease condition.",

author = "Noriyuki Kasai and Masaharu Naiki and Toshio Ariga and Yoshio Hirabayashi and Yu, {Robert K.}",

note = "Funding Information: ACNOWLEDGMENTS: NK and MN are supported Education, Science and Culture in Japan grant NS 11853 and National MS Society",

year = "1985",

month = jun,

day = "14",

doi = "10.1016/0006-291X(85)90156-1",

language = "English (US)",

volume = "129",

pages = "334--341",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Preparation and specificity of avian anti-GM2(NeuGc) ganglioside antiserum

AU - Kasai, Noriyuki

AU - Naiki, Masaharu

AU - Ariga, Toshio

AU - Hirabayashi, Yoshio

AU - Yu, Robert K.

N1 - Funding Information: ACNOWLEDGMENTS: NK and MN are supported Education, Science and Culture in Japan grant NS 11853 and National MS Society

PY - 1985/6/14

Y1 - 1985/6/14

N2 - Antibodies to N-glycolyl neuraminic acid-containing GM2 ganglioside, GM2(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassy (RIA). One(C-4) of two antisera produced did not cross-react with GM3(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM2(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM2(NeuGc) in human and animal tissues under normal and/or disease condition.

AB - Antibodies to N-glycolyl neuraminic acid-containing GM2 ganglioside, GM2(NeuGc), were prepared by immunizing chickens. The specificity of the antibodies was examined by the double immunodiffusion test and solid-phase radioimmunoassy (RIA). One(C-4) of two antisera produced did not cross-react with GM3(NeuGc) but the other(C-3) did as assessed by the double immunodiffusion test. In RIA, the antibody activity of C-4 antiserum was detected only in the IgG fraction. Specificity of the serum was examined using authentic glycolipids which were structurally related to GM2(NeuGc). The antiserum showed a high specificity for the homologous ganglioside by either an RIA or an inhibition assay. This antiserum is a useful tool for the detection of GM2(NeuGc) in human and animal tissues under normal and/or disease condition.

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