Preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by CD and NMR

Rickey Paige Hicks

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The antibiotic activity of antimicrobial peptides is generally derived via some type of disruption of the cell membrane(s). The most common models used to mimic the properties of bacterial membranes consist of mixtures of various zwitterionic and anionic phospholipids. This approach works reasonably well for Gram-positive bacteria. However, since the membranes of Gram-negative bacteria contain lipopolysaccharides, as well as zwitterionic and anionic phospholipids, a more complex model is required to simulate the outer membrane of Gram-negative bacteria. Herein we present a protocol for the preparation of models of the outer membranes of the Gram-negative bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa. This protocol can be used to prepare models of other Gram-negative bacteria provided the strain-specific lipopolysaccharides are available.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-245
Number of pages15
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1548
ISSN (Print)1064-3745

Fingerprint

Gram-Negative Bacteria
Peptides
Membranes
Lipopolysaccharides
Phospholipids
Klebsiella pneumoniae
Gram-Positive Bacteria
Pseudomonas aeruginosa
Cell Membrane
Anti-Bacterial Agents

Keywords

  • Circular dichroism
  • Gram-negative bacteria membrane models
  • Klebsiella pneumoniae
  • Lipopolysaccharides
  • Nuclear magnetic resonance
  • Phospholipids
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hicks, R. P. (2017). Preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by CD and NMR. In Methods in Molecular Biology (pp. 231-245). (Methods in Molecular Biology; Vol. 1548). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6737-7_16

Preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by CD and NMR. / Hicks, Rickey Paige.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 231-245 (Methods in Molecular Biology; Vol. 1548).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hicks, RP 2017, Preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by CD and NMR. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1548, Humana Press Inc., pp. 231-245. https://doi.org/10.1007/978-1-4939-6737-7_16
Hicks, Rickey Paige. / Preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by CD and NMR. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 231-245 (Methods in Molecular Biology).
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