Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity

Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, Wendy B. Bollag

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

In this chapter information is provided about the outer layer of the skin, the epidermis, and the predominant cells comprising this epithelium, the keratinocytes. The evidence supporting a possible role for the lipid-metabolizing enzyme phospholipase D in regulating keratinocyte differentiation is also discussed. A detailed protocol for the preparation of primary cultures of epidermal keratinocytes from neonatal mice is described, to allow other investigators to obtain data concerning these important cells involved in forming and maintaining the mechanical and water permeability of the skin. Finally, a complete protocol for monitoring phospholipase D activity in intact cells is supplied in the hope that additional research will result in a better understanding of the role of phospholipase D in controlling keratinocyte proliferation and differentiation.

Original languageEnglish (US)
Title of host publicationEpidermal Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages111-131
Number of pages21
ISBN (Print)9781493912230
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1195
ISSN (Print)1064-3745

Fingerprint

Phospholipase D
Keratinocytes
Skin
Epidermis
Permeability
Epithelium
Research Personnel
Lipids
Water
Enzymes
Research

Keywords

  • Epidermis
  • Keratinocytes
  • Mouse
  • Phosphatidic acid
  • Phosphatidylalcohol
  • Phospholipase D
  • Skin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bailey, L. J., Choudhary, V., Merai, P., & Bollag, W. B. (2014). Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity. In Epidermal Cells: Methods and Protocols (pp. 111-131). (Methods in Molecular Biology; Vol. 1195). Humana Press Inc.. https://doi.org/10.1007/7651-2014-80

Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity. / Bailey, Lakiea J.; Choudhary, Vivek; Merai, Purnima; Bollag, Wendy B.

Epidermal Cells: Methods and Protocols. Humana Press Inc., 2014. p. 111-131 (Methods in Molecular Biology; Vol. 1195).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bailey, LJ, Choudhary, V, Merai, P & Bollag, WB 2014, Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity. in Epidermal Cells: Methods and Protocols. Methods in Molecular Biology, vol. 1195, Humana Press Inc., pp. 111-131. https://doi.org/10.1007/7651-2014-80
Bailey LJ, Choudhary V, Merai P, Bollag WB. Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity. In Epidermal Cells: Methods and Protocols. Humana Press Inc. 2014. p. 111-131. (Methods in Molecular Biology). https://doi.org/10.1007/7651-2014-80
Bailey, Lakiea J. ; Choudhary, Vivek ; Merai, Purnima ; Bollag, Wendy B. / Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity. Epidermal Cells: Methods and Protocols. Humana Press Inc., 2014. pp. 111-131 (Methods in Molecular Biology).
@inbook{4bfd6e8a91134e6ea06d7839f6b0ff93,
title = "Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity",
abstract = "In this chapter information is provided about the outer layer of the skin, the epidermis, and the predominant cells comprising this epithelium, the keratinocytes. The evidence supporting a possible role for the lipid-metabolizing enzyme phospholipase D in regulating keratinocyte differentiation is also discussed. A detailed protocol for the preparation of primary cultures of epidermal keratinocytes from neonatal mice is described, to allow other investigators to obtain data concerning these important cells involved in forming and maintaining the mechanical and water permeability of the skin. Finally, a complete protocol for monitoring phospholipase D activity in intact cells is supplied in the hope that additional research will result in a better understanding of the role of phospholipase D in controlling keratinocyte proliferation and differentiation.",
keywords = "Epidermis, Keratinocytes, Mouse, Phosphatidic acid, Phosphatidylalcohol, Phospholipase D, Skin",
author = "Bailey, {Lakiea J.} and Vivek Choudhary and Purnima Merai and Bollag, {Wendy B.}",
year = "2014",
doi = "10.1007/7651-2014-80",
language = "English (US)",
isbn = "9781493912230",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "111--131",
booktitle = "Epidermal Cells",

}

TY - CHAP

T1 - Preparation of primary cultures of mouse epidermal keratinocytes and the measurement of phospholipase D activity

AU - Bailey, Lakiea J.

AU - Choudhary, Vivek

AU - Merai, Purnima

AU - Bollag, Wendy B.

PY - 2014

Y1 - 2014

N2 - In this chapter information is provided about the outer layer of the skin, the epidermis, and the predominant cells comprising this epithelium, the keratinocytes. The evidence supporting a possible role for the lipid-metabolizing enzyme phospholipase D in regulating keratinocyte differentiation is also discussed. A detailed protocol for the preparation of primary cultures of epidermal keratinocytes from neonatal mice is described, to allow other investigators to obtain data concerning these important cells involved in forming and maintaining the mechanical and water permeability of the skin. Finally, a complete protocol for monitoring phospholipase D activity in intact cells is supplied in the hope that additional research will result in a better understanding of the role of phospholipase D in controlling keratinocyte proliferation and differentiation.

AB - In this chapter information is provided about the outer layer of the skin, the epidermis, and the predominant cells comprising this epithelium, the keratinocytes. The evidence supporting a possible role for the lipid-metabolizing enzyme phospholipase D in regulating keratinocyte differentiation is also discussed. A detailed protocol for the preparation of primary cultures of epidermal keratinocytes from neonatal mice is described, to allow other investigators to obtain data concerning these important cells involved in forming and maintaining the mechanical and water permeability of the skin. Finally, a complete protocol for monitoring phospholipase D activity in intact cells is supplied in the hope that additional research will result in a better understanding of the role of phospholipase D in controlling keratinocyte proliferation and differentiation.

KW - Epidermis

KW - Keratinocytes

KW - Mouse

KW - Phosphatidic acid

KW - Phosphatidylalcohol

KW - Phospholipase D

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=84907330867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907330867&partnerID=8YFLogxK

U2 - 10.1007/7651-2014-80

DO - 10.1007/7651-2014-80

M3 - Chapter

AN - SCOPUS:84907330867

SN - 9781493912230

T3 - Methods in Molecular Biology

SP - 111

EP - 131

BT - Epidermal Cells

PB - Humana Press Inc.

ER -