Presence of DNA sequence homologous to RAS gene in the aquatic fungus Achlya ambisexualis: Possible role in sporangiogenesis

Elias K. Manavathu, Kalachar Suryanarayana, Seyed E. Hasnain, Wai Choi Leung

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Abstract

The water mold Achlya ambisexualis reproduces asexually by the formation of zoospores produced in sporangia. The differentiation of vegetative hyphae to sporangia involves a series of morphologic and biochemical changes. Thus far, very little is known about the regulation of sporogenesis in this organism. Using DNA-DNA and DNA-RNA hybridizations, we investigated the presence of DNA sequences homologous to the yeast RAS gene in A. ambisexualis. A 1.8-kilobase (kb)HindIII fragment containing the complete RASsc1 gene specifically hybridized to BamHI, PstI, and SalI digested A. ambisexualis DNA. RNA blot analysis revealed the presence of a detectable level of RNA transcript (1.3 kb) that hybridizes to RASsc1, only in differentiating mycelia. In addition, the35S-labeled polypeptide profile of sporulating hyphae differs from that of vegetative hyphae. The selective transcription of 1.3-kb RNA during sporangiogenesis indicates that it may play some significant role in the asexual sporulation in A. ambisexualis.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalCurrent Microbiology
Volume17
Issue number4
DOIs
StatePublished - Jul 1 1988

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ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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