Presence of DNA sequence homologous to RAS gene in the aquatic fungus Achlya ambisexualis

Possible role in sporangiogenesis

Elias K. Manavathu, Kalachar Suryanarayana, Seyed E. Hasnain, Wai Choi Leung

Research output: Contribution to journalArticle

Abstract

The water mold Achlya ambisexualis reproduces asexually by the formation of zoospores produced in sporangia. The differentiation of vegetative hyphae to sporangia involves a series of morphologic and biochemical changes. Thus far, very little is known about the regulation of sporogenesis in this organism. Using DNA-DNA and DNA-RNA hybridizations, we investigated the presence of DNA sequences homologous to the yeast RAS gene in A. ambisexualis. A 1.8-kilobase (kb)HindIII fragment containing the complete RASsc1 gene specifically hybridized to BamHI, PstI, and SalI digested A. ambisexualis DNA. RNA blot analysis revealed the presence of a detectable level of RNA transcript (1.3 kb) that hybridizes to RASsc1, only in differentiating mycelia. In addition, the35S-labeled polypeptide profile of sporulating hyphae differs from that of vegetative hyphae. The selective transcription of 1.3-kb RNA during sporangiogenesis indicates that it may play some significant role in the asexual sporulation in A. ambisexualis.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalCurrent Microbiology
Volume17
Issue number4
DOIs
StatePublished - Jul 1 1988

Fingerprint

Achlya
Hyphae
Fungi
Sporangia
RNA
DNA
Genes
Mycelium
Yeasts
Peptides
Water

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Presence of DNA sequence homologous to RAS gene in the aquatic fungus Achlya ambisexualis : Possible role in sporangiogenesis. / Manavathu, Elias K.; Suryanarayana, Kalachar; Hasnain, Seyed E.; Leung, Wai Choi.

In: Current Microbiology, Vol. 17, No. 4, 01.07.1988, p. 183-187.

Research output: Contribution to journalArticle

Manavathu, Elias K. ; Suryanarayana, Kalachar ; Hasnain, Seyed E. ; Leung, Wai Choi. / Presence of DNA sequence homologous to RAS gene in the aquatic fungus Achlya ambisexualis : Possible role in sporangiogenesis. In: Current Microbiology. 1988 ; Vol. 17, No. 4. pp. 183-187.
@article{065abe58e2a64410a4a92377de72b1b8,
title = "Presence of DNA sequence homologous to RAS gene in the aquatic fungus Achlya ambisexualis: Possible role in sporangiogenesis",
abstract = "The water mold Achlya ambisexualis reproduces asexually by the formation of zoospores produced in sporangia. The differentiation of vegetative hyphae to sporangia involves a series of morphologic and biochemical changes. Thus far, very little is known about the regulation of sporogenesis in this organism. Using DNA-DNA and DNA-RNA hybridizations, we investigated the presence of DNA sequences homologous to the yeast RAS gene in A. ambisexualis. A 1.8-kilobase (kb)HindIII fragment containing the complete RASsc1 gene specifically hybridized to BamHI, PstI, and SalI digested A. ambisexualis DNA. RNA blot analysis revealed the presence of a detectable level of RNA transcript (1.3 kb) that hybridizes to RASsc1, only in differentiating mycelia. In addition, the35S-labeled polypeptide profile of sporulating hyphae differs from that of vegetative hyphae. The selective transcription of 1.3-kb RNA during sporangiogenesis indicates that it may play some significant role in the asexual sporulation in A. ambisexualis.",
author = "Manavathu, {Elias K.} and Kalachar Suryanarayana and Hasnain, {Seyed E.} and Leung, {Wai Choi}",
year = "1988",
month = "7",
day = "1",
doi = "10.1007/BF01589449",
language = "English (US)",
volume = "17",
pages = "183--187",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Presence of DNA sequence homologous to RAS gene in the aquatic fungus Achlya ambisexualis

T2 - Possible role in sporangiogenesis

AU - Manavathu, Elias K.

AU - Suryanarayana, Kalachar

AU - Hasnain, Seyed E.

AU - Leung, Wai Choi

PY - 1988/7/1

Y1 - 1988/7/1

N2 - The water mold Achlya ambisexualis reproduces asexually by the formation of zoospores produced in sporangia. The differentiation of vegetative hyphae to sporangia involves a series of morphologic and biochemical changes. Thus far, very little is known about the regulation of sporogenesis in this organism. Using DNA-DNA and DNA-RNA hybridizations, we investigated the presence of DNA sequences homologous to the yeast RAS gene in A. ambisexualis. A 1.8-kilobase (kb)HindIII fragment containing the complete RASsc1 gene specifically hybridized to BamHI, PstI, and SalI digested A. ambisexualis DNA. RNA blot analysis revealed the presence of a detectable level of RNA transcript (1.3 kb) that hybridizes to RASsc1, only in differentiating mycelia. In addition, the35S-labeled polypeptide profile of sporulating hyphae differs from that of vegetative hyphae. The selective transcription of 1.3-kb RNA during sporangiogenesis indicates that it may play some significant role in the asexual sporulation in A. ambisexualis.

AB - The water mold Achlya ambisexualis reproduces asexually by the formation of zoospores produced in sporangia. The differentiation of vegetative hyphae to sporangia involves a series of morphologic and biochemical changes. Thus far, very little is known about the regulation of sporogenesis in this organism. Using DNA-DNA and DNA-RNA hybridizations, we investigated the presence of DNA sequences homologous to the yeast RAS gene in A. ambisexualis. A 1.8-kilobase (kb)HindIII fragment containing the complete RASsc1 gene specifically hybridized to BamHI, PstI, and SalI digested A. ambisexualis DNA. RNA blot analysis revealed the presence of a detectable level of RNA transcript (1.3 kb) that hybridizes to RASsc1, only in differentiating mycelia. In addition, the35S-labeled polypeptide profile of sporulating hyphae differs from that of vegetative hyphae. The selective transcription of 1.3-kb RNA during sporangiogenesis indicates that it may play some significant role in the asexual sporulation in A. ambisexualis.

UR - http://www.scopus.com/inward/record.url?scp=0023740980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023740980&partnerID=8YFLogxK

U2 - 10.1007/BF01589449

DO - 10.1007/BF01589449

M3 - Article

VL - 17

SP - 183

EP - 187

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 4

ER -