TY - JOUR
T1 - Presentation of antagonist peptides to naive CD4+ T cells abrogates spatial reorganization of class II MHC peptide complexes on the surface of dendritic cells
AU - Chmielowski, Bartosz
AU - Pacholczyk, Rafal
AU - Kraj, Piotr
AU - Kisielow, Pawel
AU - Ignatowicz, Leszek
PY - 2002/11/12
Y1 - 2002/11/12
N2 - By using dendritic cells (DCs) transduced with retroviruses encoding covalent Abβ/peptide fusion proteins tagged with fluorescent proteins, we followed the relocation of class II MHC molecules loaded with agonist or null peptides during the onset of activation of naive and effector CD4+ T cells. Clusters of T cell receptor (TCR)/CD3 complex formed in parallel with clusters of agonist class II MHC/peptide complexes on the surface of DCs. However, activation of naive but not effector T cells was accompanied by expulsion of the null class II MHC/peptide complexes from the T cell-DC interface. These effects were perturbed in the presence of exogenously supplied antagonist peptide. These results suggest that interference with selective relocation of agonist and null MHC/peptide complexes in the immunological synapse contributes to the inhibitory effect of antagonist peptides on the response of naive CD4+ T cells to agonist ligands.
AB - By using dendritic cells (DCs) transduced with retroviruses encoding covalent Abβ/peptide fusion proteins tagged with fluorescent proteins, we followed the relocation of class II MHC molecules loaded with agonist or null peptides during the onset of activation of naive and effector CD4+ T cells. Clusters of T cell receptor (TCR)/CD3 complex formed in parallel with clusters of agonist class II MHC/peptide complexes on the surface of DCs. However, activation of naive but not effector T cells was accompanied by expulsion of the null class II MHC/peptide complexes from the T cell-DC interface. These effects were perturbed in the presence of exogenously supplied antagonist peptide. These results suggest that interference with selective relocation of agonist and null MHC/peptide complexes in the immunological synapse contributes to the inhibitory effect of antagonist peptides on the response of naive CD4+ T cells to agonist ligands.
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U2 - 10.1073/pnas.222463499
DO - 10.1073/pnas.222463499
M3 - Article
C2 - 12411579
AN - SCOPUS:0037069369
SN - 0027-8424
VL - 99
SP - 15012
EP - 15017
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23
ER -