TY - JOUR
T1 - Preventing bone loss and weight gain with combinations of vitamin d and phytochemicals
AU - Lai, Ching Yi
AU - Yang, Jeong Yeh
AU - Rayalam, Srujana
AU - Della-Fera, Mary Anne
AU - Ambati, Suresh
AU - Lewis, Richard D.
AU - Hamrick, Mark W.
AU - Hartzell, Diane L.
AU - Baile, Clifton A.
PY - 2011/11/1
Y1 - 2011/11/1
N2 - Vitamin D and certain natural compounds have been shown to regulate both lipid metabolism and bone formation. Treatments that prevent or reverse age-related increase in bone marrow adiposity could both increase new bone formation and inhibit bone destruction. We tested the hypothesis that dietary supplementation with combinations of vitamin D and phytochemicals inhibits bone loss and decreases adiposity to a greater extent than control or vitamin D-alone diets. Aged ovariectomized female rats (12 months old, n=50, initial body weight=240g) were given control (AIN-93M diet), vitamin D (2,400IU/kg), or vitamin D plus resveratrol (16, 80, or 400mg/kg of diet [low, medium, and high dose, respectively]), quercetin (80, 400, or 2,000mg/kg of diet), and genistein (64, 256, or 1,040mg/kg of diet) for 8 weeks. The high-dose treatment (vitamin D+400mg/kg resveratrol+2,000mg/kg quercetin+1,040mg/kg genistein) reduced body weight gain (P<.05) and the fat pad weights (P<.05). This treatment also increased the serum concentration of insulin-like growth factor-1 (P<.05) and the bone mineral content of the femur. Micro-computed tomography and histomorphometric analyses indicated that the high-dose treatment prevented loss of trabecular bone (P<.05) and reduced marrow adipocytes (P<.001) and osteoclasts (P<.05) compared with the control and vitamin D alone (P<.05). We conclude that aged ovariectomized female rats supplemented with vitamin D combined with genistein, quercetin, and resveratrol had improved bone mineral density and reduced body weight gain and a significant decrease in bone marrow adipocytes. The synergistic effects of a combination of phytochemicals with vitamin D may be effective in reducing bone loss and weight gain after menopause.
AB - Vitamin D and certain natural compounds have been shown to regulate both lipid metabolism and bone formation. Treatments that prevent or reverse age-related increase in bone marrow adiposity could both increase new bone formation and inhibit bone destruction. We tested the hypothesis that dietary supplementation with combinations of vitamin D and phytochemicals inhibits bone loss and decreases adiposity to a greater extent than control or vitamin D-alone diets. Aged ovariectomized female rats (12 months old, n=50, initial body weight=240g) were given control (AIN-93M diet), vitamin D (2,400IU/kg), or vitamin D plus resveratrol (16, 80, or 400mg/kg of diet [low, medium, and high dose, respectively]), quercetin (80, 400, or 2,000mg/kg of diet), and genistein (64, 256, or 1,040mg/kg of diet) for 8 weeks. The high-dose treatment (vitamin D+400mg/kg resveratrol+2,000mg/kg quercetin+1,040mg/kg genistein) reduced body weight gain (P<.05) and the fat pad weights (P<.05). This treatment also increased the serum concentration of insulin-like growth factor-1 (P<.05) and the bone mineral content of the femur. Micro-computed tomography and histomorphometric analyses indicated that the high-dose treatment prevented loss of trabecular bone (P<.05) and reduced marrow adipocytes (P<.001) and osteoclasts (P<.05) compared with the control and vitamin D alone (P<.05). We conclude that aged ovariectomized female rats supplemented with vitamin D combined with genistein, quercetin, and resveratrol had improved bone mineral density and reduced body weight gain and a significant decrease in bone marrow adipocytes. The synergistic effects of a combination of phytochemicals with vitamin D may be effective in reducing bone loss and weight gain after menopause.
KW - bone loss
KW - genistein
KW - osteoporosis
KW - ovariectomized rats
KW - quercetin
KW - resveratrol
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U2 - 10.1089/jmf.2010.0232
DO - 10.1089/jmf.2010.0232
M3 - Article
C2 - 21663481
AN - SCOPUS:80755175258
SN - 1096-620X
VL - 14
SP - 1352
EP - 1362
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 11
ER -