Abstract
AimsWe determined the role of the Rho kinase (ROCK) isoforms in diabetes-induced vascular endothelial dysfunction and enhancement of arginase activity and expression.Methods and resultsStudies were performed in aortic tissues from haplo-insufficient (H-I) ROCK1 and ROCK2 mice and wild-type (WT) mice rendered diabetic with streptozotocin and in bovine aortic endothelial cells (BAECs) treated with high glucose (HG, 25 mM). Protein expression of both ROCK isoforms was substantially elevated in aortas of WT mice after 8 weeks of diabetes and in BAECs after 48 h in HG. Impairment of endothelium-dependent vasorelaxation of aortas was observed in diabetic WT mice. However, there was no impairment in aortas of diabetic ROCK1 H-I mice and less impairment in aortas of diabetic ROCK2 H-I mice, compared with non-diabetic mice. These vascular effects were associated with the prevention of diabetes-induced decrease in nitric oxide (NO) production and a rise in arginase activity/expression. Acute treatment with the arginase inhibitor, BEC, improved endothelium-dependent vasorelaxation of aortas of both diabetic WT and ROCK2, but not of ROCK1 mice.ConclusionPartial deletion of either ROCK isoform, but to a greater extent ROCK1, attenuates diabetes-induced vascular endothelial dysfunction by preventing increased arginase activity and expression and reduction in NO production in type 1 diabetes. Limiting ROCK and arginase activity improves vascular function in diabetes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 509-519 |
| Number of pages | 11 |
| Journal | Cardiovascular Research |
| Volume | 97 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2013 |
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Keywords
- Arginase
- Isoforms
- Rho kinase
- Type 1 diabetes
- Vascular function
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
Prevention of diabetes-induced arginase activation and vascular dysfunction by Rho kinase (ROCK) knockout. / Yao, Lin; Chandra, Surabhi; Toque, Haroldo A.; Bhatta, Anil; Rojas, Modesto; Caldwell, Ruth B.; Caldwell, R. William.
In: Cardiovascular Research, Vol. 97, No. 3, 01.03.2013, p. 509-519.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevention of diabetes-induced arginase activation and vascular dysfunction by Rho kinase (ROCK) knockout
AU - Yao, Lin
AU - Chandra, Surabhi
AU - Toque, Haroldo A.
AU - Bhatta, Anil
AU - Rojas, Modesto
AU - Caldwell, Ruth B.
AU - Caldwell, R. William
PY - 2013/3/1
Y1 - 2013/3/1
N2 - AimsWe determined the role of the Rho kinase (ROCK) isoforms in diabetes-induced vascular endothelial dysfunction and enhancement of arginase activity and expression.Methods and resultsStudies were performed in aortic tissues from haplo-insufficient (H-I) ROCK1 and ROCK2 mice and wild-type (WT) mice rendered diabetic with streptozotocin and in bovine aortic endothelial cells (BAECs) treated with high glucose (HG, 25 mM). Protein expression of both ROCK isoforms was substantially elevated in aortas of WT mice after 8 weeks of diabetes and in BAECs after 48 h in HG. Impairment of endothelium-dependent vasorelaxation of aortas was observed in diabetic WT mice. However, there was no impairment in aortas of diabetic ROCK1 H-I mice and less impairment in aortas of diabetic ROCK2 H-I mice, compared with non-diabetic mice. These vascular effects were associated with the prevention of diabetes-induced decrease in nitric oxide (NO) production and a rise in arginase activity/expression. Acute treatment with the arginase inhibitor, BEC, improved endothelium-dependent vasorelaxation of aortas of both diabetic WT and ROCK2, but not of ROCK1 mice.ConclusionPartial deletion of either ROCK isoform, but to a greater extent ROCK1, attenuates diabetes-induced vascular endothelial dysfunction by preventing increased arginase activity and expression and reduction in NO production in type 1 diabetes. Limiting ROCK and arginase activity improves vascular function in diabetes.
AB - AimsWe determined the role of the Rho kinase (ROCK) isoforms in diabetes-induced vascular endothelial dysfunction and enhancement of arginase activity and expression.Methods and resultsStudies were performed in aortic tissues from haplo-insufficient (H-I) ROCK1 and ROCK2 mice and wild-type (WT) mice rendered diabetic with streptozotocin and in bovine aortic endothelial cells (BAECs) treated with high glucose (HG, 25 mM). Protein expression of both ROCK isoforms was substantially elevated in aortas of WT mice after 8 weeks of diabetes and in BAECs after 48 h in HG. Impairment of endothelium-dependent vasorelaxation of aortas was observed in diabetic WT mice. However, there was no impairment in aortas of diabetic ROCK1 H-I mice and less impairment in aortas of diabetic ROCK2 H-I mice, compared with non-diabetic mice. These vascular effects were associated with the prevention of diabetes-induced decrease in nitric oxide (NO) production and a rise in arginase activity/expression. Acute treatment with the arginase inhibitor, BEC, improved endothelium-dependent vasorelaxation of aortas of both diabetic WT and ROCK2, but not of ROCK1 mice.ConclusionPartial deletion of either ROCK isoform, but to a greater extent ROCK1, attenuates diabetes-induced vascular endothelial dysfunction by preventing increased arginase activity and expression and reduction in NO production in type 1 diabetes. Limiting ROCK and arginase activity improves vascular function in diabetes.
KW - Arginase
KW - Isoforms
KW - Rho kinase
KW - Type 1 diabetes
KW - Vascular function
UR - http://www.scopus.com/inward/record.url?scp=84874266181&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874266181&partnerID=8YFLogxK
U2 - 10.1093/cvr/cvs371
DO - 10.1093/cvr/cvs371
M3 - Article
C2 - 23250919
AN - SCOPUS:84874266181
VL - 97
SP - 509
EP - 519
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 3
ER -