Prevention of mammary tumorigenesis by intermittent caloric restriction: Does caloric intake during refeeding modulate the response?

Margot P. Cleary, Xin Hu, Michael E. Grossmann, Subhash C. Juneja, Soner Dogan, Joseph P. Grande, Nita Jane Maihle

Research output: Contribution to journalArticle

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Abstract

Chronic caloric restriction (CCR) prevents mammary tumorigenesis in rodents, but a protective effect for intermittent caloric restriction (ICR) is less well documented. We recently reported that ICR reduced mammary tumor (MT) incidence of mouse mammary tumor virus-transforming growth factor (MMTV-TGF)-α mice to a greater extent than did CCR. Here, we repeated this protocol and obtained serum and tissue samples. Ad libitum (AL) MMTV-TGF-α mice were fed AIN-93M diet. Beginning at 10 weeks of age, ICR mice received isocaloric AIN-93M-mod diet (2-fold increases in protein, fat, vitamins, and minerals) at 50% of ad libitum for 3 weeks followed by 3 weeks refeeding with AIN-93M diet. CCR mice were pair-fed AIN-93M:AIN-93M-mod (2:1) matching intakes for restriction/refeeding cycles. Mice were sacrificed for MT size, at 79 (end of 12th restriction) or at 80 (1 week after 12th refeeding) weeks of age. AL and ICR-80 mice had heavier body weights than ICR-79 and CCR mice (P < 0.0001). Cumulative food intakes of ICR and CCR mice were reduced 12% and 15% versus AL mice (P < 0.0001). However, ICR mice consumed significantly (P < 0.0001) more food than did AL mice during refeeding. MT incidence was 84%, 13%, and 27% for AL, ICR, and CCR mice, respectively. MT weight (P < 0.0011) and number (P < 0.01) were higher for AL mice compared with ICR and CCR mice. AL and ICR-80 mice had similar serum IGF-I levels, but only AL values were higher than those of ICR-79 and CCR mice (P < 0.0017). ICR mice had more MT DNA breaks compared with AL and CCR mice, suggesting enhanced apoptosis (P < 0.02). AL mice had higher mammary fat pad ObR and ObRb leptin receptor mRNA expression than did ICR and CCR mice (P < 0.001), but there was no effect on MTs. These results confirm that ICR prevents development of MTs to a greater extent than does CCR, although "overeating" during refeeding may compromise this protection.

Original languageEnglish (US)
Pages (from-to)70-80
Number of pages11
JournalExperimental Biology and Medicine
Volume232
Issue number1
StatePublished - Jan 1 2007
Externally publishedYes

Fingerprint

Caloric Restriction
Energy Intake
Carcinogenesis
Breast
Tumors
Breast Neoplasms
Nutrition
Mouse mammary tumor virus
Transforming Growth Factors
Diet
Viruses

Keywords

  • Apoptosis
  • Caloric restriction
  • IGF-I
  • Leptin receptor
  • Mammary tumors
  • Mice
  • Prevention
  • Weight gain
  • Weight loss

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cleary, M. P., Hu, X., Grossmann, M. E., Juneja, S. C., Dogan, S., Grande, J. P., & Maihle, N. J. (2007). Prevention of mammary tumorigenesis by intermittent caloric restriction: Does caloric intake during refeeding modulate the response? Experimental Biology and Medicine, 232(1), 70-80.

Prevention of mammary tumorigenesis by intermittent caloric restriction : Does caloric intake during refeeding modulate the response? / Cleary, Margot P.; Hu, Xin; Grossmann, Michael E.; Juneja, Subhash C.; Dogan, Soner; Grande, Joseph P.; Maihle, Nita Jane.

In: Experimental Biology and Medicine, Vol. 232, No. 1, 01.01.2007, p. 70-80.

Research output: Contribution to journalArticle

Cleary, MP, Hu, X, Grossmann, ME, Juneja, SC, Dogan, S, Grande, JP & Maihle, NJ 2007, 'Prevention of mammary tumorigenesis by intermittent caloric restriction: Does caloric intake during refeeding modulate the response?', Experimental Biology and Medicine, vol. 232, no. 1, pp. 70-80.
Cleary, Margot P. ; Hu, Xin ; Grossmann, Michael E. ; Juneja, Subhash C. ; Dogan, Soner ; Grande, Joseph P. ; Maihle, Nita Jane. / Prevention of mammary tumorigenesis by intermittent caloric restriction : Does caloric intake during refeeding modulate the response?. In: Experimental Biology and Medicine. 2007 ; Vol. 232, No. 1. pp. 70-80.
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