Abstract
Purpose: This in vitro study evaluated the amount and distribution of outward fluid flow that occurred when an experimental etch-and-rinse hydrophobic adhesive was applied to ethanol-saturated dentin before and after oxalate pretreatment. Materials and Methods: Measurements of dentin permeability were performed under a constant pulpal pressure of 20 cm H2O in deep and middle dentin. A lucifer yellow solution was placed in the pulp chamber to determine the distribution of the water contamination of the hybrid layers. Results: The distribution of fluorescence in dentin specimens that were not pretreated with oxalate revealed that the dye permeated around the resin tags and filled the hybrid layer. Dentin specimens pretreated with oxalate prior to resin bonding, showed 80% to 83% less (p < 0.05) water contamination compared to controls. The dentin permeability results obtained before and after oxalate pretreatment showed that oxalate decreased dentin permeability by 98% (p < 0.05) compared to acid-etched controls. This prevented outward fluid movement during bonding, resulting in better resin sealing of dentin due to the formation of a double seal of resin tags over calcium oxalate crystals in the tubules. Conclusion: Outward dentinal fluid flow may contaminate hybrid layers during adhesive bonding procedures. Pretreatment of acid-etched dentin with 3% oxalic acid prior to bonding procedures can prevent outward fluid flow during bonding and water contamination of the hydrophobic hybrid layers. Keywords: water contamination, two-photon confocal microscopy, ethanol-saturated dentin, hydrophobic hybrid layer, dentin permeability.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 271-278 |
| Number of pages | 8 |
| Journal | Journal of Adhesive Dentistry |
| Volume | 11 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 2009 |
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ASJC Scopus subject areas
- Orthodontics
- Oral Surgery
- Periodontics
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Prevention of water contamination of ethanol-saturated dentin and hydrophobic hybrid layers. / Sauro, Salvatore; Watson, Timothy F.; Mannocci, Francesco; Tay, Franklin R.; Pashley, David H.
In: Journal of Adhesive Dentistry, Vol. 11, No. 4, 01.01.2009, p. 271-278.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevention of water contamination of ethanol-saturated dentin and hydrophobic hybrid layers
AU - Sauro, Salvatore
AU - Watson, Timothy F.
AU - Mannocci, Francesco
AU - Tay, Franklin R.
AU - Pashley, David H.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Purpose: This in vitro study evaluated the amount and distribution of outward fluid flow that occurred when an experimental etch-and-rinse hydrophobic adhesive was applied to ethanol-saturated dentin before and after oxalate pretreatment. Materials and Methods: Measurements of dentin permeability were performed under a constant pulpal pressure of 20 cm H2O in deep and middle dentin. A lucifer yellow solution was placed in the pulp chamber to determine the distribution of the water contamination of the hybrid layers. Results: The distribution of fluorescence in dentin specimens that were not pretreated with oxalate revealed that the dye permeated around the resin tags and filled the hybrid layer. Dentin specimens pretreated with oxalate prior to resin bonding, showed 80% to 83% less (p < 0.05) water contamination compared to controls. The dentin permeability results obtained before and after oxalate pretreatment showed that oxalate decreased dentin permeability by 98% (p < 0.05) compared to acid-etched controls. This prevented outward fluid movement during bonding, resulting in better resin sealing of dentin due to the formation of a double seal of resin tags over calcium oxalate crystals in the tubules. Conclusion: Outward dentinal fluid flow may contaminate hybrid layers during adhesive bonding procedures. Pretreatment of acid-etched dentin with 3% oxalic acid prior to bonding procedures can prevent outward fluid flow during bonding and water contamination of the hydrophobic hybrid layers. Keywords: water contamination, two-photon confocal microscopy, ethanol-saturated dentin, hydrophobic hybrid layer, dentin permeability.
AB - Purpose: This in vitro study evaluated the amount and distribution of outward fluid flow that occurred when an experimental etch-and-rinse hydrophobic adhesive was applied to ethanol-saturated dentin before and after oxalate pretreatment. Materials and Methods: Measurements of dentin permeability were performed under a constant pulpal pressure of 20 cm H2O in deep and middle dentin. A lucifer yellow solution was placed in the pulp chamber to determine the distribution of the water contamination of the hybrid layers. Results: The distribution of fluorescence in dentin specimens that were not pretreated with oxalate revealed that the dye permeated around the resin tags and filled the hybrid layer. Dentin specimens pretreated with oxalate prior to resin bonding, showed 80% to 83% less (p < 0.05) water contamination compared to controls. The dentin permeability results obtained before and after oxalate pretreatment showed that oxalate decreased dentin permeability by 98% (p < 0.05) compared to acid-etched controls. This prevented outward fluid movement during bonding, resulting in better resin sealing of dentin due to the formation of a double seal of resin tags over calcium oxalate crystals in the tubules. Conclusion: Outward dentinal fluid flow may contaminate hybrid layers during adhesive bonding procedures. Pretreatment of acid-etched dentin with 3% oxalic acid prior to bonding procedures can prevent outward fluid flow during bonding and water contamination of the hydrophobic hybrid layers. Keywords: water contamination, two-photon confocal microscopy, ethanol-saturated dentin, hydrophobic hybrid layer, dentin permeability.
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UR - http://www.scopus.com/inward/citedby.url?scp=76849112902&partnerID=8YFLogxK
U2 - 10.3290/j.jad.a17073
DO - 10.3290/j.jad.a17073
M3 - Article
C2 - 19701507
AN - SCOPUS:76849112902
VL - 11
SP - 271
EP - 278
JO - The journal of adhesive dentistry
JF - The journal of adhesive dentistry
SN - 1461-5185
IS - 4
ER -