Prevention of water contamination of ethanol-saturated dentin and hydrophobic hybrid layers

Salvatore Sauro, Timothy F. Watson, Francesco Mannocci, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Purpose: This in vitro study evaluated the amount and distribution of outward fluid flow that occurred when an experimental etch-and-rinse hydrophobic adhesive was applied to ethanol-saturated dentin before and after oxalate pretreatment. Materials and Methods: Measurements of dentin permeability were performed under a constant pulpal pressure of 20 cm H2O in deep and middle dentin. A lucifer yellow solution was placed in the pulp chamber to determine the distribution of the water contamination of the hybrid layers. Results: The distribution of fluorescence in dentin specimens that were not pretreated with oxalate revealed that the dye permeated around the resin tags and filled the hybrid layer. Dentin specimens pretreated with oxalate prior to resin bonding, showed 80% to 83% less (p < 0.05) water contamination compared to controls. The dentin permeability results obtained before and after oxalate pretreatment showed that oxalate decreased dentin permeability by 98% (p < 0.05) compared to acid-etched controls. This prevented outward fluid movement during bonding, resulting in better resin sealing of dentin due to the formation of a double seal of resin tags over calcium oxalate crystals in the tubules. Conclusion: Outward dentinal fluid flow may contaminate hybrid layers during adhesive bonding procedures. Pretreatment of acid-etched dentin with 3% oxalic acid prior to bonding procedures can prevent outward fluid flow during bonding and water contamination of the hydrophobic hybrid layers. Keywords: water contamination, two-photon confocal microscopy, ethanol-saturated dentin, hydrophobic hybrid layer, dentin permeability.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
JournalJournal of Adhesive Dentistry
Volume11
Issue number4
DOIs
StatePublished - Jan 1 2009

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Dentin
Dentin Permeability
Oxalates
Ethanol
Water
Adhesives
Dentinal Fluid
Oxalic Acid
Calcium Oxalate
Acids
Dental Pulp Cavity
Photons
Confocal Microscopy
Coloring Agents
Fluorescence
Pressure

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics

Cite this

Prevention of water contamination of ethanol-saturated dentin and hydrophobic hybrid layers. / Sauro, Salvatore; Watson, Timothy F.; Mannocci, Francesco; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Journal of Adhesive Dentistry, Vol. 11, No. 4, 01.01.2009, p. 271-278.

Research output: Contribution to journalArticle

Sauro, Salvatore ; Watson, Timothy F. ; Mannocci, Francesco ; Tay, Franklin Chi Meng ; Pashley, David Henry. / Prevention of water contamination of ethanol-saturated dentin and hydrophobic hybrid layers. In: Journal of Adhesive Dentistry. 2009 ; Vol. 11, No. 4. pp. 271-278.
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abstract = "Purpose: This in vitro study evaluated the amount and distribution of outward fluid flow that occurred when an experimental etch-and-rinse hydrophobic adhesive was applied to ethanol-saturated dentin before and after oxalate pretreatment. Materials and Methods: Measurements of dentin permeability were performed under a constant pulpal pressure of 20 cm H2O in deep and middle dentin. A lucifer yellow solution was placed in the pulp chamber to determine the distribution of the water contamination of the hybrid layers. Results: The distribution of fluorescence in dentin specimens that were not pretreated with oxalate revealed that the dye permeated around the resin tags and filled the hybrid layer. Dentin specimens pretreated with oxalate prior to resin bonding, showed 80{\%} to 83{\%} less (p < 0.05) water contamination compared to controls. The dentin permeability results obtained before and after oxalate pretreatment showed that oxalate decreased dentin permeability by 98{\%} (p < 0.05) compared to acid-etched controls. This prevented outward fluid movement during bonding, resulting in better resin sealing of dentin due to the formation of a double seal of resin tags over calcium oxalate crystals in the tubules. Conclusion: Outward dentinal fluid flow may contaminate hybrid layers during adhesive bonding procedures. Pretreatment of acid-etched dentin with 3{\%} oxalic acid prior to bonding procedures can prevent outward fluid flow during bonding and water contamination of the hydrophobic hybrid layers. Keywords: water contamination, two-photon confocal microscopy, ethanol-saturated dentin, hydrophobic hybrid layer, dentin permeability.",
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