Primary structure and functional characteristics of a mammalian sodium- coupled high affinity dicarboxylate transporter

Ramesh Kekuda, Haiping Wang, Wei Huang, Ana M. Pajor, Frederick H. Leibach, Lawrence D Devoe, Puttur D Prasad, Vadivel Ganapathy

Research output: Contribution to journalArticle

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Abstract

We have cloned a Na+-dependent, high affinity dicarboxylate transporter (NaDC3) from rat placenta. NaDC3 exhibits 48% identity in amino acid sequence with rat NaDC1, a Na+-dependent, low affinity dicarboxylate transporter. NaDC3-specific mRNA is detectable in kidney, brain, liver, and placenta. When expressed in mammalian cells, NaDC3 mediates Na+-dependent transport of succinate with a K(t) of 2 μM. The transport function of NaDC3 shows a sigmoidal relationship with regard to Na+ concentration, with a Hill coefficient of 2.7. NaDC3 accepts a number of dicarboxylates including dimethylsuccinate as substrates and excludes monocarboxylates. Li+ inhibits NaDC3 in the presence of Na+. Transport of succinate by NaDC3 is markedly influenced by pH, the transport function gradually decreasing when pH is acidified from 8.0 to 5.5. In contrast, the influence of pH on NaDC3-mediated transport of citrate is biphasic in which a pH change from 8.0 to 6.5 stimulates the transport and any further acidification inhibits the transport. In addition, the potency of citrate to compete with NaDC3-mediated transport of succinate increases 25-fold when pH is changed from 7.5 to 5.5. These data show that NaDC3 interacts preferentially with the divalent anionic species of citrate. This represents the first report on the cloning and functional characterization of a mammalian Na+-dependent, high affinity dicarboxylate transporter.

Original languageEnglish (US)
Pages (from-to)3422-3429
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number6
DOIs
StatePublished - Feb 5 1999

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Dicarboxylic Acid Transporters
Succinic Acid
Citric Acid
Sodium
Rats
Acidification
Cloning
Placenta
Liver
Brain
Cells
Amino Acids
Messenger RNA
Substrates
Organism Cloning
Amino Acid Sequence
Kidney

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Primary structure and functional characteristics of a mammalian sodium- coupled high affinity dicarboxylate transporter. / Kekuda, Ramesh; Wang, Haiping; Huang, Wei; Pajor, Ana M.; Leibach, Frederick H.; Devoe, Lawrence D; Prasad, Puttur D; Ganapathy, Vadivel.

In: Journal of Biological Chemistry, Vol. 274, No. 6, 05.02.1999, p. 3422-3429.

Research output: Contribution to journalArticle

Kekuda, Ramesh ; Wang, Haiping ; Huang, Wei ; Pajor, Ana M. ; Leibach, Frederick H. ; Devoe, Lawrence D ; Prasad, Puttur D ; Ganapathy, Vadivel. / Primary structure and functional characteristics of a mammalian sodium- coupled high affinity dicarboxylate transporter. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 6. pp. 3422-3429.
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