Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris

Xiu Yun Ding, Chun Hai Li, Yun Feng Zhu, Li Ya Sun, Chunhong Yan, Li Xin Zhang, Dong Xiao Feng, Gao Ming Chen

Research output: Contribution to journalArticle

Abstract

Matrix metalloproteinase-2 (MMP-2) plays important role in tumor invasion and metastasis. MMP-2 is expressed in yeast expression system for further studying its function mechanism. The expression sequence of MMP-2 (gelatinase A) have been obtained by PCR amplifying, restriction enzyme cut and sequencing analysis demostrate that the sequence is correct. The expression vector pPIC9/GelA containing gelatinase A sequence is constructed and transformed the yeast Pichia pastoris. The gelatin enzymography demostrate that recombinant protein secreted by transformant yeast is capable of degrading gelatin and the molecular weight of the matrix metalloproteinase-2 is 66 ku in SDS-PAGE.

Original languageEnglish (US)
Pages (from-to)293-294
Number of pages2
JournalProgress in Biochemistry and Biophysics
Volume26
Issue number3
StatePublished - Dec 1 1999
Externally publishedYes

Fingerprint

Pichia
Matrix Metalloproteinase 2
Yeast
Yeasts
Gelatin
Recombinant Proteins
Sequence Analysis
Tumors
Polyacrylamide Gel Electrophoresis
Molecular Weight
Molecular weight
Neoplasm Metastasis
Polymerase Chain Reaction
Enzymes
Neoplasms

Keywords

  • Expression
  • Matrix metalloproteinase-2
  • Pichia pastoris

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Ding, X. Y., Li, C. H., Zhu, Y. F., Sun, L. Y., Yan, C., Zhang, L. X., ... Chen, G. M. (1999). Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris. Progress in Biochemistry and Biophysics, 26(3), 293-294.

Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris. / Ding, Xiu Yun; Li, Chun Hai; Zhu, Yun Feng; Sun, Li Ya; Yan, Chunhong; Zhang, Li Xin; Feng, Dong Xiao; Chen, Gao Ming.

In: Progress in Biochemistry and Biophysics, Vol. 26, No. 3, 01.12.1999, p. 293-294.

Research output: Contribution to journalArticle

Ding, XY, Li, CH, Zhu, YF, Sun, LY, Yan, C, Zhang, LX, Feng, DX & Chen, GM 1999, 'Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris', Progress in Biochemistry and Biophysics, vol. 26, no. 3, pp. 293-294.
Ding, Xiu Yun ; Li, Chun Hai ; Zhu, Yun Feng ; Sun, Li Ya ; Yan, Chunhong ; Zhang, Li Xin ; Feng, Dong Xiao ; Chen, Gao Ming. / Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris. In: Progress in Biochemistry and Biophysics. 1999 ; Vol. 26, No. 3. pp. 293-294.
@article{84b3d44869434394acde3c39373b445a,
title = "Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris",
abstract = "Matrix metalloproteinase-2 (MMP-2) plays important role in tumor invasion and metastasis. MMP-2 is expressed in yeast expression system for further studying its function mechanism. The expression sequence of MMP-2 (gelatinase A) have been obtained by PCR amplifying, restriction enzyme cut and sequencing analysis demostrate that the sequence is correct. The expression vector pPIC9/GelA containing gelatinase A sequence is constructed and transformed the yeast Pichia pastoris. The gelatin enzymography demostrate that recombinant protein secreted by transformant yeast is capable of degrading gelatin and the molecular weight of the matrix metalloproteinase-2 is 66 ku in SDS-PAGE.",
keywords = "Expression, Matrix metalloproteinase-2, Pichia pastoris",
author = "Ding, {Xiu Yun} and Li, {Chun Hai} and Zhu, {Yun Feng} and Sun, {Li Ya} and Chunhong Yan and Zhang, {Li Xin} and Feng, {Dong Xiao} and Chen, {Gao Ming}",
year = "1999",
month = "12",
day = "1",
language = "English (US)",
volume = "26",
pages = "293--294",
journal = "Progress in Biochemistry and Biophysics",
issn = "1000-3282",
publisher = "Science Press",
number = "3",

}

TY - JOUR

T1 - Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris

AU - Ding, Xiu Yun

AU - Li, Chun Hai

AU - Zhu, Yun Feng

AU - Sun, Li Ya

AU - Yan, Chunhong

AU - Zhang, Li Xin

AU - Feng, Dong Xiao

AU - Chen, Gao Ming

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Matrix metalloproteinase-2 (MMP-2) plays important role in tumor invasion and metastasis. MMP-2 is expressed in yeast expression system for further studying its function mechanism. The expression sequence of MMP-2 (gelatinase A) have been obtained by PCR amplifying, restriction enzyme cut and sequencing analysis demostrate that the sequence is correct. The expression vector pPIC9/GelA containing gelatinase A sequence is constructed and transformed the yeast Pichia pastoris. The gelatin enzymography demostrate that recombinant protein secreted by transformant yeast is capable of degrading gelatin and the molecular weight of the matrix metalloproteinase-2 is 66 ku in SDS-PAGE.

AB - Matrix metalloproteinase-2 (MMP-2) plays important role in tumor invasion and metastasis. MMP-2 is expressed in yeast expression system for further studying its function mechanism. The expression sequence of MMP-2 (gelatinase A) have been obtained by PCR amplifying, restriction enzyme cut and sequencing analysis demostrate that the sequence is correct. The expression vector pPIC9/GelA containing gelatinase A sequence is constructed and transformed the yeast Pichia pastoris. The gelatin enzymography demostrate that recombinant protein secreted by transformant yeast is capable of degrading gelatin and the molecular weight of the matrix metalloproteinase-2 is 66 ku in SDS-PAGE.

KW - Expression

KW - Matrix metalloproteinase-2

KW - Pichia pastoris

UR - http://www.scopus.com/inward/record.url?scp=21944448312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21944448312&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:21944448312

VL - 26

SP - 293

EP - 294

JO - Progress in Biochemistry and Biophysics

JF - Progress in Biochemistry and Biophysics

SN - 1000-3282

IS - 3

ER -