Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders

Qiuhua Yang, Qian Ma, Jiean Xu, Zhiping Liu, Jianqiu Zou, Jian Shen, Yaqi Zhou, Qingen Da, Xiaoxiao Mao, Sarah Lu, David J Fulton, Neal L Weintraub, Zsolt Bagi, Mei Hong, Yuqing Huo

Research output: Contribution to journalArticlepeer-review

Abstract

Myeloid cells, including monocytes/macrophages, primarily rely on glucose and lipid metabolism to provide the energy and metabolites needed for their functions and survival. AMP-activated protein kinase (AMPK, its gene is PRKA for human, Prka for rodent) is a key metabolic sensor that regulates many metabolic pathways. We studied recruitment and viability of Prkaa1-deficient myeloid cells in mice and the phenotype of these mice in the context of cardio-metabolic diseases. We found that the deficiency of Prkaa1 in myeloid cells downregulated genes for glucose and lipid metabolism, compromised glucose and lipid metabolism of macrophages, and suppressed their recruitment to adipose, liver and arterial vessel walls. The viability of macrophages in the above tissues/organs was also decreased. These cellular alterations resulted in decreases in body weight, insulin resistance, and lipid accumulation in liver of mice fed with a high fat diet, and reduced the size of atherosclerotic lesions of mice fed with a Western diet. Our results indicate that AMPKα1/PRKAA1-regulated metabolism supports monocyte recruitment and macrophage viability, contributing to the development of diet-induced metabolic disorders including diabetes and atherosclerosis.

Original languageEnglish (US)
Article number611354
Pages (from-to)611354
JournalFrontiers in Cell and Developmental Biology
Volume8
DOIs
StatePublished - 2020

Keywords

  • AMPKα1/PRKAA1
  • glycolysis
  • macrophage viability
  • metabolic disorders
  • monocyte recruitment

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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