Pro-apoptotic effect of fly ash leachates in hepatocytes of freshwater fish (Channa punctata Bloch)

Mehboob Ali, Shakilur Rahman, Hasibur Rehman, Kanchan Bhatia, Rizwan A. Ansari, Sheikh Raisuddin

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The pro-apoptotic effect of fly ash leachates (FAL) was studied in the hepatocytes of an Indian freshwater fish, Channa punctata Bloch. Hepatocytes were exposed to different concentrations of '7-day' FAL for 24 and 48 h and various parameters of apoptosis were studied using standardized procedures. FAL-induced apoptosis in hepatocytes was indicated by cytological examination, DNA fragmentation and DNA laddering. The induction in cytochrome-c release, caspases 3, 7, 10 and 9 activities and lactate dehydrogenase level provide mechanistic platform for FAL-induced apoptosis. Cytological examination showed an unambiguous apoptotic effect of ash leachates in fish hepatocytes. Exposed hepatocytes also showed increased production of H2O2, superoxide ions and an increase in lipid peroxidation (LPO). The present study suggests a possible role of reactive oxygen species (ROS) in FAL-induced apoptosis in hepatocytes. Lactate dehydrogenase, LPO and apoptosis as biomarkers of cytotoxicity have recently been used for assessment of ecotoxicological impact of environmental chemicals. Our findings show that these biomarkers may also be used for evaluation of ecotoxicological impact of complex chemical mixture such as fly ash and its leachates.

Original languageEnglish (US)
Pages (from-to)63-71
Number of pages9
JournalToxicology in Vitro
Issue number1
StatePublished - Feb 2007
Externally publishedYes


  • Apoptosis
  • Biomarkers
  • Caspases
  • Fish hepatocytes
  • Fly ash leachates
  • Reactive oxygen species

ASJC Scopus subject areas

  • Toxicology


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