TY - JOUR
T1 - Production of pyrimidine dimers in DNA of human skin exposed in situ to UVA radiation
AU - Freeman, Steven E.
AU - Gange, Richard W.
AU - Sutherland, John C.
AU - Matzinger, Ezra A.
AU - Sutherland, Betsy M.
N1 - Funding Information:
Manuscript received May 15, 1986; accepted for publication September 25. 1986. This investigation was supported by the Office of Health and Environm ental Research of the U.S. Department of E nergy and by N atio nal Institutes of Health grants CA 26492 and CA 23096 to BMS, and by a grant from the Arthur O. and Gullan M. Wellman Foundation. *Data presented in preliminary form: Freeman SE. Gange RW, Matzinger EA. Sutherland JC, Sutherland BM: Production of pyrimidine dimers in skin of humans exposed to UVA. Photochcl11 Photobiol 41: 90S. 1985. t Recipient of a fellowship frolll a National Cancer Institute training grant CA 09121. :j: Present address: Lovelace Medical Foundation. Research Division, 2425 Hidgecrest Drive, S.E .• Albuquerque. New Mexico 87108. Reprint requests to: Betsy M. Sutherland. Ph. D., Departmcnt of Biology, Brookhavcn National Laboratory, Upton. New York 11973. Abbreviatio ns: ESS: Microco((lIs III/clls UV endonuclease-sensitive sites ESS/kb: ESS per 1000 bases L,,: nUl11ber average molecular length PilE: photoreactivating enzyme UVA: 320-400 nl11 U V 13: 290-320 nll1
PY - 1987/4
Y1 - 1987/4
N2 - Cyclobutyl pyrimidine dimers, measured as sites recognized by the dimer-specific ultraviolet (UV) endonuclease from Micrococcus luteus, were produced in DNA of human skin exposed in situ to UVA (320-400 nm) radiation. The dimer yields produced by a broadband UVA source, by broadband UVA filtered to remove all light of wavelength less than 340 nm, and by narrow band radiation centered at 365 nm were similar, indicating that UVA radiation, and not stray shorter wavelength radiation, was responsible for dimer production. The identity of the UVA-induced DNA lesions was confirmed as pyrimidine dimers by photoreactivation of approximately 100% of the endonuclease-sensitive sites in vitro with the 40,000 dalton Escherichia coli photoreactivating enzyme.
AB - Cyclobutyl pyrimidine dimers, measured as sites recognized by the dimer-specific ultraviolet (UV) endonuclease from Micrococcus luteus, were produced in DNA of human skin exposed in situ to UVA (320-400 nm) radiation. The dimer yields produced by a broadband UVA source, by broadband UVA filtered to remove all light of wavelength less than 340 nm, and by narrow band radiation centered at 365 nm were similar, indicating that UVA radiation, and not stray shorter wavelength radiation, was responsible for dimer production. The identity of the UVA-induced DNA lesions was confirmed as pyrimidine dimers by photoreactivation of approximately 100% of the endonuclease-sensitive sites in vitro with the 40,000 dalton Escherichia coli photoreactivating enzyme.
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U2 - 10.1111/1523-1747.ep12469778
DO - 10.1111/1523-1747.ep12469778
M3 - Article
C2 - 3559269
AN - SCOPUS:0023113867
VL - 88
SP - 430
EP - 433
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 4
ER -