Abstract
Cyclobutyl pyrimidine dimers, measured as sites recognized by the dimer-specific ultraviolet (UV) endonuclease from Micrococcus luteus, were produced in DNA of human skin exposed in situ to UVA (320-400 nm) radiation. The dimer yields produced by a broadband UVA source, by broadband UVA filtered to remove all light of wavelength less than 340 nm, and by narrow band radiation centered at 365 nm were similar, indicating that UVA radiation, and not stray shorter wavelength radiation, was responsible for dimer production. The identity of the UVA-induced DNA lesions was confirmed as pyrimidine dimers by photoreactivation of approximately 100% of the endonuclease-sensitive sites in vitro with the 40,000 dalton Escherichia coli photoreactivating enzyme.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 430-433 |
| Number of pages | 4 |
| Journal | Journal of Investigative Dermatology |
| Volume | 88 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology
- Cell Biology
Cite this
Production of pyrimidine dimers in DNA of human skin exposed in situ to UVA radiation. / Freeman, Steven E.; Gange, Richard W.; Sutherland, John C.; Matzinger, Ezra A.; Sutherland, Betsy M.
In: Journal of Investigative Dermatology, Vol. 88, No. 4, 04.1987, p. 430-433.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of pyrimidine dimers in DNA of human skin exposed in situ to UVA radiation
AU - Freeman, Steven E.
AU - Gange, Richard W.
AU - Sutherland, John C.
AU - Matzinger, Ezra A.
AU - Sutherland, Betsy M.
PY - 1987/4
Y1 - 1987/4
N2 - Cyclobutyl pyrimidine dimers, measured as sites recognized by the dimer-specific ultraviolet (UV) endonuclease from Micrococcus luteus, were produced in DNA of human skin exposed in situ to UVA (320-400 nm) radiation. The dimer yields produced by a broadband UVA source, by broadband UVA filtered to remove all light of wavelength less than 340 nm, and by narrow band radiation centered at 365 nm were similar, indicating that UVA radiation, and not stray shorter wavelength radiation, was responsible for dimer production. The identity of the UVA-induced DNA lesions was confirmed as pyrimidine dimers by photoreactivation of approximately 100% of the endonuclease-sensitive sites in vitro with the 40,000 dalton Escherichia coli photoreactivating enzyme.
AB - Cyclobutyl pyrimidine dimers, measured as sites recognized by the dimer-specific ultraviolet (UV) endonuclease from Micrococcus luteus, were produced in DNA of human skin exposed in situ to UVA (320-400 nm) radiation. The dimer yields produced by a broadband UVA source, by broadband UVA filtered to remove all light of wavelength less than 340 nm, and by narrow band radiation centered at 365 nm were similar, indicating that UVA radiation, and not stray shorter wavelength radiation, was responsible for dimer production. The identity of the UVA-induced DNA lesions was confirmed as pyrimidine dimers by photoreactivation of approximately 100% of the endonuclease-sensitive sites in vitro with the 40,000 dalton Escherichia coli photoreactivating enzyme.
UR - http://www.scopus.com/inward/record.url?scp=0023113867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023113867&partnerID=8YFLogxK
U2 - 10.1111/1523-1747.ep12469778
DO - 10.1111/1523-1747.ep12469778
M3 - Article
C2 - 3559269
AN - SCOPUS:0023113867
VL - 88
SP - 430
EP - 433
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 4
ER -