Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model

Ganapathy Rajaseger, Chin Leong Lim, Koon Wui Lee, Pachiappan Arjunan, Lu Jia, Shabbir Moochhala

Research output: Contribution to journalArticle

Abstract

Heat induced complications cause an increase in a large number of proteins which play a role in diverse pathways during heat shock. A detailed characterization of these proteins is essential for understanding the molecular mechanisms involved in heat stroke. In this report, the proteins present in rat liver were compared at 37 °C (control) and at core temperature (Tc) 42 °C (heat stress) by 1D PAGE and MALDI/MS/MS. Among proteins identified in the sample after heat stress are dimethyglycine dehydrogenase, transketolase, carboxylic ester hydrolase, pyruvate kinase, L-type pyruvate kinase, arginosuccinate synthetase; fumarylacetoacetate hydrolase and peptidylpropyl isomerase A. These findings show that analysis of large scale proteins by MALDI/MS/MS provides a better understanding of the molecular mechanisms associated with heat shock. The resolution of proteins examined by 1D-PAGE was less than that obtained with 2D-PAGE. More specifically, 2D-PAGE allows better identification of low molecular weight proteins that can not be resolved by 1D-PAGE.

Original languageEnglish (US)
Pages (from-to)2924-2928
Number of pages5
JournalFrontiers in Bioscience
Volume11
Issue numberSUPPL. 3
DOIs
StatePublished - Jun 5 2006
Externally publishedYes

Keywords

  • 1D PAGE
  • Heat stress
  • Hepatocellular proteins
  • MALDI
  • MS
  • Polyacrylamide gel electrophoresis
  • Rat model

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model'. Together they form a unique fingerprint.

  • Cite this

    Rajaseger, G., Lim, C. L., Lee, K. W., Arjunan, P., Jia, L., & Moochhala, S. (2006). Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model. Frontiers in Bioscience, 11(SUPPL. 3), 2924-2928. https://doi.org/10.2741/2021