Prognostic significance of BAG-1 expression in nonsmall cell lung cancer

Stewart Rorke, Sean Murphy, Mahmoud Khalifa, Garry Chernenko, Shou-Ching Tang

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the expression of BAG-1 in a cohort of patients with nonsmall cell lung cancer (NSCLC). The intensity and subcellular distribution of BAG-1 expression were correlated with overall survival. Tumor samples were collected from 85 patients diagnosed with NSCLC between 1993-1995 in St. John's, Newfoundland. Expression of BAG-1 was determined by immunohistochemistry using polyclonal anti-BAG-1 antibody. There was significant variation in the immunohistochemical staining patterns of BAG-1, including nonstaining and staining of either the cytoplasm, nucleus or both. Univariate Cox regression analysis showed that those patients whose tumor overexpressed BAG-1 had a significant reduction in the risk of death (hazard ratio = 0.53, p = 0.03). The survival advantage of patients with BAG-1 overexpression tumor was also demonstrated by Kaplan-Meier analysis and log-rank tests (median survival 30.10 months versus 17.04 months, p = 0.05). In addition, multivariate Cox regression analysis showed that patients whose tumor exhibited intense cytoplasmic staining had a further reduction of the risk of death (hazard ratio = 0.42, p = 0.03) and this effect was independent of age, stage and histology. All stages were included in the analysis. Our preliminary data strongly indicate that further investigation is warranted to better define the role of BAG-1 as an independent prognostic factor in NSCLC.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalInternational Journal of Cancer
Volume95
Issue number5
DOIs
StatePublished - Sep 20 2001

Fingerprint

Non-Small Cell Lung Carcinoma
Risk Reduction Behavior
Staining and Labeling
Survival
Neoplasms
Regression Analysis
Newfoundland and Labrador
Kaplan-Meier Estimate
Histology
Cytoplasm
Immunohistochemistry
Antibodies

Keywords

  • Apoptosis
  • BAG-1/RAP46
  • Lung cancer
  • Prognosis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Prognostic significance of BAG-1 expression in nonsmall cell lung cancer. / Rorke, Stewart; Murphy, Sean; Khalifa, Mahmoud; Chernenko, Garry; Tang, Shou-Ching.

In: International Journal of Cancer, Vol. 95, No. 5, 20.09.2001, p. 317-322.

Research output: Contribution to journalArticle

Rorke, Stewart ; Murphy, Sean ; Khalifa, Mahmoud ; Chernenko, Garry ; Tang, Shou-Ching. / Prognostic significance of BAG-1 expression in nonsmall cell lung cancer. In: International Journal of Cancer. 2001 ; Vol. 95, No. 5. pp. 317-322.
@article{55941a8efec348fca9f8121d83efa7d7,
title = "Prognostic significance of BAG-1 expression in nonsmall cell lung cancer",
abstract = "The purpose of this study was to evaluate the expression of BAG-1 in a cohort of patients with nonsmall cell lung cancer (NSCLC). The intensity and subcellular distribution of BAG-1 expression were correlated with overall survival. Tumor samples were collected from 85 patients diagnosed with NSCLC between 1993-1995 in St. John's, Newfoundland. Expression of BAG-1 was determined by immunohistochemistry using polyclonal anti-BAG-1 antibody. There was significant variation in the immunohistochemical staining patterns of BAG-1, including nonstaining and staining of either the cytoplasm, nucleus or both. Univariate Cox regression analysis showed that those patients whose tumor overexpressed BAG-1 had a significant reduction in the risk of death (hazard ratio = 0.53, p = 0.03). The survival advantage of patients with BAG-1 overexpression tumor was also demonstrated by Kaplan-Meier analysis and log-rank tests (median survival 30.10 months versus 17.04 months, p = 0.05). In addition, multivariate Cox regression analysis showed that patients whose tumor exhibited intense cytoplasmic staining had a further reduction of the risk of death (hazard ratio = 0.42, p = 0.03) and this effect was independent of age, stage and histology. All stages were included in the analysis. Our preliminary data strongly indicate that further investigation is warranted to better define the role of BAG-1 as an independent prognostic factor in NSCLC.",
keywords = "Apoptosis, BAG-1/RAP46, Lung cancer, Prognosis",
author = "Stewart Rorke and Sean Murphy and Mahmoud Khalifa and Garry Chernenko and Shou-Ching Tang",
year = "2001",
month = "9",
day = "20",
doi = "10.1002/1097-0215(20010920)95:5<317::AID-IJC1055>3.0.CO;2-J",
language = "English (US)",
volume = "95",
pages = "317--322",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Prognostic significance of BAG-1 expression in nonsmall cell lung cancer

AU - Rorke, Stewart

AU - Murphy, Sean

AU - Khalifa, Mahmoud

AU - Chernenko, Garry

AU - Tang, Shou-Ching

PY - 2001/9/20

Y1 - 2001/9/20

N2 - The purpose of this study was to evaluate the expression of BAG-1 in a cohort of patients with nonsmall cell lung cancer (NSCLC). The intensity and subcellular distribution of BAG-1 expression were correlated with overall survival. Tumor samples were collected from 85 patients diagnosed with NSCLC between 1993-1995 in St. John's, Newfoundland. Expression of BAG-1 was determined by immunohistochemistry using polyclonal anti-BAG-1 antibody. There was significant variation in the immunohistochemical staining patterns of BAG-1, including nonstaining and staining of either the cytoplasm, nucleus or both. Univariate Cox regression analysis showed that those patients whose tumor overexpressed BAG-1 had a significant reduction in the risk of death (hazard ratio = 0.53, p = 0.03). The survival advantage of patients with BAG-1 overexpression tumor was also demonstrated by Kaplan-Meier analysis and log-rank tests (median survival 30.10 months versus 17.04 months, p = 0.05). In addition, multivariate Cox regression analysis showed that patients whose tumor exhibited intense cytoplasmic staining had a further reduction of the risk of death (hazard ratio = 0.42, p = 0.03) and this effect was independent of age, stage and histology. All stages were included in the analysis. Our preliminary data strongly indicate that further investigation is warranted to better define the role of BAG-1 as an independent prognostic factor in NSCLC.

AB - The purpose of this study was to evaluate the expression of BAG-1 in a cohort of patients with nonsmall cell lung cancer (NSCLC). The intensity and subcellular distribution of BAG-1 expression were correlated with overall survival. Tumor samples were collected from 85 patients diagnosed with NSCLC between 1993-1995 in St. John's, Newfoundland. Expression of BAG-1 was determined by immunohistochemistry using polyclonal anti-BAG-1 antibody. There was significant variation in the immunohistochemical staining patterns of BAG-1, including nonstaining and staining of either the cytoplasm, nucleus or both. Univariate Cox regression analysis showed that those patients whose tumor overexpressed BAG-1 had a significant reduction in the risk of death (hazard ratio = 0.53, p = 0.03). The survival advantage of patients with BAG-1 overexpression tumor was also demonstrated by Kaplan-Meier analysis and log-rank tests (median survival 30.10 months versus 17.04 months, p = 0.05). In addition, multivariate Cox regression analysis showed that patients whose tumor exhibited intense cytoplasmic staining had a further reduction of the risk of death (hazard ratio = 0.42, p = 0.03) and this effect was independent of age, stage and histology. All stages were included in the analysis. Our preliminary data strongly indicate that further investigation is warranted to better define the role of BAG-1 as an independent prognostic factor in NSCLC.

KW - Apoptosis

KW - BAG-1/RAP46

KW - Lung cancer

KW - Prognosis

UR - http://www.scopus.com/inward/record.url?scp=0035922110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035922110&partnerID=8YFLogxK

U2 - 10.1002/1097-0215(20010920)95:5<317::AID-IJC1055>3.0.CO;2-J

DO - 10.1002/1097-0215(20010920)95:5<317::AID-IJC1055>3.0.CO;2-J

M3 - Article

VL - 95

SP - 317

EP - 322

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 5

ER -