Progressing Preemptive Genotyping of CYP2C19 Allelic Variants for Sickle Cell Disease Patients

Cheedy Jaja, Nadine Barrett, Niren Patel, Matt Lyon, Hongyan Xu, Abdullah Kutlar

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Aims: Interindividual variability in drug response and adverse effects have been described for proton pump inhibitors, anticonvulsants, selective serotonin reuptake inhibitors, tricyclic antidepressants, and anti-infectives, but little is known about the safety and efficacy of these medications in patients with sickle cell disease (SCD). We genotyped the CYP2C19 gene which has been implicated in the metabolism of these drugs in an SCD patient cohort to determine the frequencies of reduced function, increased function, or complete loss-of-function variants. Materials and Methods: DNAs from 165 unrelated SCD patients were genotyped for nine CYP2C19 (∗2, ∗3, ∗4, ∗5, ∗6, ∗7,∗8, ∗12, and ∗17) alleles using the iPLEX® ADME PGx multiplex panel. Results: Three CYP2C19 alleles (∗2, ∗12, and ∗17) were detected with the following frequencies: 0.209, 0.006, and 0.236, respectively. The predicted phenotype frequencies were distributed as extensive (31.5%), intermediate (24.8%), poor (5.5%), ultrarapid (30.3%), and unknown metabolizers (7.9%). Discussion: Prognostic genotyping is potentially useful for identifying SCD patients with allelic variants linked to proven clinical pharmacokinetic consequences for several drugs metabolized by the CYP2C19 gene. However, the main challenge to implementing a genetics-guided prescribing practice is ensuring concordance between CYP2C19 genotypes and metabolic phenotypes in SCD patients.

Original languageEnglish (US)
Pages (from-to)609-615
Number of pages7
JournalGenetic Testing and Molecular Biomarkers
Volume20
Issue number10
DOIs
StatePublished - Oct 1 2016

Keywords

  • drug metabolizing enzyme
  • pharmacogenetics
  • precision medicine
  • preemptive genotyping
  • sickle cell disease

ASJC Scopus subject areas

  • Genetics(clinical)

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