Promotion of plasma membrane repair by vitamin e

Amber C. Howard, Anna K. McNeil, Paul L McNeil

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Severe vitamin E deficiency results in lethal myopathy in animal models. Membrane repair is an important myocyte response to plasma membrane disruption injury as when repair fails, myocytes die and muscular dystrophy ensues. Here we show that supplementation of cultured cells with α-tocopherol, the most common form of vitamin E, promotes plasma membrane repair. Conversely, in the absence of α-tocopherol supplementation, exposure of cultured cells to an oxidant challenge strikingly inhibits repair. Comparative measurements reveal that, to promote repair, an anti-oxidant must associate with membranes, as α-tocopherol does, or be capable of α-tocopherol regeneration. Finally, we show that myocytes in intact muscle cannot repair membranes when exposed to an oxidant challenge, but show enhanced repair when supplemented with vitamin E. Our work suggests a novel biological function for vitamin E in promoting myocyte plasma membrane repair. We propose that this function is essential for maintenance of skeletal muscle homeostasis.

Original languageEnglish (US)
Article number597
JournalNature Communications
Volume2
Issue number1
DOIs
StatePublished - Dec 29 2011

Fingerprint

tocopherol
vitamins
Tocopherols
promotion
Cell membranes
Vitamins
Muscle Cells
Repair
Vitamin E
Cell Membrane
Oxidants
membranes
muscle cells
Membranes
Cultured Cells
Vitamin E Deficiency
Muscular Dystrophies
Muscular Diseases
cultured cells
Muscle

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Howard, A. C., McNeil, A. K., & McNeil, P. L. (2011). Promotion of plasma membrane repair by vitamin e. Nature Communications, 2(1), [597]. https://doi.org/10.1038/ncomms1594

Promotion of plasma membrane repair by vitamin e. / Howard, Amber C.; McNeil, Anna K.; McNeil, Paul L.

In: Nature Communications, Vol. 2, No. 1, 597, 29.12.2011.

Research output: Contribution to journalArticle

Howard, Amber C. ; McNeil, Anna K. ; McNeil, Paul L. / Promotion of plasma membrane repair by vitamin e. In: Nature Communications. 2011 ; Vol. 2, No. 1.
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