TY - JOUR
T1 - Properties of porcine and recombinant human collagen matrices for optically clear tissue engineering applications
AU - Liu, Y.
AU - Griffith, M.
AU - Watsky, M. A.
AU - Forrester, J. V.
AU - Kuffová, L.
AU - Grant, D.
AU - Merrett, K.
AU - Carlsson, Dave J.
PY - 2006/6
Y1 - 2006/6
N2 - Porcine and recombinant human atelocollagen I solutions were cross-linked with a water soluble carbodiimide at vanous stoichiometries and collagen concentrations (5-20 w/w %). The resulting hydrogels were clear and, when used as cell growth matrices, allowed cell and nerve visualization in vitro and in vivo. We have previously reported that, after six months of implantation in pigs' and rabbits' corneas, these robust hydrogels allowed regeneration of host cells and nerves to give optically clear corneas with no detected loss in thickness, indicating stable engraftment. Here, the biocompatible hydrogel formulations leading to this novel in vivo performance were characterized for amine consumption, gel hydration, thermal properties, optical clarity, refractive index, nutrient diffusion, biodegradation, tensile measurements, and average pore diameters. Gels with excellent in vitro (epithelial overgrowth, neurite penetration) and in vivo performance (clarity, touch sensitivity regeneration) had 4-11 nm pores, yet had glucose and albumin diffusive coefficients similar to mammalian corneas and allowed neurite extension through the gels.
AB - Porcine and recombinant human atelocollagen I solutions were cross-linked with a water soluble carbodiimide at vanous stoichiometries and collagen concentrations (5-20 w/w %). The resulting hydrogels were clear and, when used as cell growth matrices, allowed cell and nerve visualization in vitro and in vivo. We have previously reported that, after six months of implantation in pigs' and rabbits' corneas, these robust hydrogels allowed regeneration of host cells and nerves to give optically clear corneas with no detected loss in thickness, indicating stable engraftment. Here, the biocompatible hydrogel formulations leading to this novel in vivo performance were characterized for amine consumption, gel hydration, thermal properties, optical clarity, refractive index, nutrient diffusion, biodegradation, tensile measurements, and average pore diameters. Gels with excellent in vitro (epithelial overgrowth, neurite penetration) and in vivo performance (clarity, touch sensitivity regeneration) had 4-11 nm pores, yet had glucose and albumin diffusive coefficients similar to mammalian corneas and allowed neurite extension through the gels.
UR - http://www.scopus.com/inward/record.url?scp=33745615427&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745615427&partnerID=8YFLogxK
U2 - 10.1021/bm060160o
DO - 10.1021/bm060160o
M3 - Article
C2 - 16768403
AN - SCOPUS:33745615427
SN - 1525-7797
VL - 7
SP - 1819
EP - 1828
JO - Biomacromolecules
JF - Biomacromolecules
IS - 6
ER -