Properties of porcine and recombinant human collagen matrices for optically clear tissue engineering applications

Y. Liu, M. Griffith, Mitchell Aaron Watsky, J. V. Forrester, L. Kuffová, D. Grant, K. Merrett, Dave J. Carlsson

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Porcine and recombinant human atelocollagen I solutions were cross-linked with a water soluble carbodiimide at vanous stoichiometries and collagen concentrations (5-20 w/w %). The resulting hydrogels were clear and, when used as cell growth matrices, allowed cell and nerve visualization in vitro and in vivo. We have previously reported that, after six months of implantation in pigs' and rabbits' corneas, these robust hydrogels allowed regeneration of host cells and nerves to give optically clear corneas with no detected loss in thickness, indicating stable engraftment. Here, the biocompatible hydrogel formulations leading to this novel in vivo performance were characterized for amine consumption, gel hydration, thermal properties, optical clarity, refractive index, nutrient diffusion, biodegradation, tensile measurements, and average pore diameters. Gels with excellent in vitro (epithelial overgrowth, neurite penetration) and in vivo performance (clarity, touch sensitivity regeneration) had 4-11 nm pores, yet had glucose and albumin diffusive coefficients similar to mammalian corneas and allowed neurite extension through the gels.

Original languageEnglish (US)
Pages (from-to)1819-1828
Number of pages10
JournalBiomacromolecules
Volume7
Issue number6
DOIs
StatePublished - Jan 1 2006

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Tissue engineering
Collagen
Hydrogels
Gels
Carbodiimides
Hydrogel
Cell growth
Biodegradation
Stoichiometry
Hydration
Nutrients
Amines
Glucose
Albumins
Refractive index
Thermodynamic properties
Visualization
Water

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Properties of porcine and recombinant human collagen matrices for optically clear tissue engineering applications. / Liu, Y.; Griffith, M.; Watsky, Mitchell Aaron; Forrester, J. V.; Kuffová, L.; Grant, D.; Merrett, K.; Carlsson, Dave J.

In: Biomacromolecules, Vol. 7, No. 6, 01.01.2006, p. 1819-1828.

Research output: Contribution to journalArticle

Liu, Y, Griffith, M, Watsky, MA, Forrester, JV, Kuffová, L, Grant, D, Merrett, K & Carlsson, DJ 2006, 'Properties of porcine and recombinant human collagen matrices for optically clear tissue engineering applications', Biomacromolecules, vol. 7, no. 6, pp. 1819-1828. https://doi.org/10.1021/bm060160o
Liu, Y. ; Griffith, M. ; Watsky, Mitchell Aaron ; Forrester, J. V. ; Kuffová, L. ; Grant, D. ; Merrett, K. ; Carlsson, Dave J. / Properties of porcine and recombinant human collagen matrices for optically clear tissue engineering applications. In: Biomacromolecules. 2006 ; Vol. 7, No. 6. pp. 1819-1828.
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