Prostaglandin E2potentiates interleukin-1β induced interleukin-6 production by human gingival fibroblasts

Charlene Ann Czuszak

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Increased levels of cytokines and prostanoids have been detected in inflamed gingival tissue and may play an important role in periodontal pathogenesis. Recent studies suggest that monocytic products, such as interleukin (IL)-1β, could stimulate IL-6 production by human gingival fibroblasts (HGF). In this context, the production of local cytokines and inflammatory mediators could regulate the secretory capacity of resident gingival fibroblasts. Therefore, the purpose of this study was to determine if PGE2 induced by IL-1β could potentiate the IL-6 response by HGF. Utilizing an ELISA, it was determined that maximal IL-6 occurred when HGF were stimulated with 0.10-10 nM IL-1β. These concentrations of IL-1β also induced a small, but significant increase in PGE2 production by HGF. Interestingly, the combination of ILγβ and PGE2 induced a synergistic rise in IL-6 production by HGF. Moreover, inclusion of indomethacin caused a 20% reduction in IL-6 production and totally eliminated PGE2 production. These findings provide additional rationale for the clinical use of NSAIDs in the management of periodontal disease due to their ability to attenuate production of both PGE2, and IL-6. These results suggest the endogenous PGE2 induced by IL-1β plays an important regulatory role in IL 6 production by HGF. Moreover, they support the concept that elevated PGE2induced during inflammation can regulate HGF secretory function.

Original languageEnglish (US)
Pages (from-to)635-640
Number of pages6
JournalJournal of Clinical Periodontology
Volume23
Issue number7
DOIs
StatePublished - Jan 1 1996

Fingerprint

Interleukin-1
Prostaglandins
Interleukin-6
Fibroblasts
Dinoprostone
Cytokines
Interleukins
Periodontal Diseases
Non-Steroidal Anti-Inflammatory Agents
Indomethacin
Enzyme-Linked Immunosorbent Assay
Inflammation

Keywords

  • Gingival fibroblasts
  • Indomethacin
  • Inflammation
  • Interleukin-1β
  • Interleukin-6
  • Periodontitis
  • Prostaglandin-E

ASJC Scopus subject areas

  • Periodontics

Cite this

Prostaglandin E2potentiates interleukin-1β induced interleukin-6 production by human gingival fibroblasts. / Czuszak, Charlene Ann.

In: Journal of Clinical Periodontology, Vol. 23, No. 7, 01.01.1996, p. 635-640.

Research output: Contribution to journalArticle

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