Protection against HeMA-induced mitochondrial injury in vitro by Nrf2 activation

Yang Jiao, Tao Niu, Huan Liu, Franklin Chi Meng Tay, Ji hua Chen

Research output: Contribution to journalArticle

Abstract

Dental resin monomers such as 2-hydroxyethyl methacrylate (HEMA) disturb vital cell functions and induce mitochondrial intrinsic apoptosis via generation of oxidative stress. Nuclear factor erythroid 2-related factor 2 (Nrf2) regulates the gene expression of antioxidative enzymes and plays a crucial role in the maintenance of cellular redox equilibrium and mitochondrial homeostasis. The present study investigated the functional significance of Nrf2 in cellular response toward HEMA. It was found that HEMA stimulation promoted nuclear translocation of Nrf2 and increased Nrf2 and heme oxygenase-1 (HO-1) expression, which was further enhanced by Nrf2 activator tert-butylhydroquinone (tBHQ), but suppressed by Nrf2 inhibitor ML385. Pretreatment of primary human dental pulp cells (hDPCs) with tBHQ protected the cells from HEMA-induced oxidative injury (increased reactive oxygen species production and apoptosis) and mitochondrial impairment (morphological alterations, decreased ATP production, suppressed oxidative phosphorylation activity, depolarization of mitochondrial membrane potential, and disrupted electron transport chain). In contrast, pretreatment with ML385 increased cell sensitivity to these injurious processes. This protective effect on mitochondria could be related to peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α)/nuclear respiratory factor 1 (NRF1) pathway. These results contribute to the understanding of the function of Nrf2 and the development of novel therapies to counteract the adverse effects of dental resin monomers.

Original languageEnglish (US)
Article number3501059
JournalOxidative medicine and cellular longevity
Volume2019
DOIs
StatePublished - Jan 1 2019

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Chemical activation
Synthetic Resins
Wounds and Injuries
Explosive well stimulation
Nuclear Respiratory Factor 1
Monomers
Apoptosis
Dental Pulp
Peroxisome Proliferator-Activated Receptors
Heme Oxygenase-1
Mitochondria
Oxidative stress
Deciduous Tooth
Mitochondrial Membrane Potential
Oxidative Phosphorylation
Depolarization
Electron Transport
Gene expression
Oxidation-Reduction
Pulp

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Cell Biology

Cite this

Protection against HeMA-induced mitochondrial injury in vitro by Nrf2 activation. / Jiao, Yang; Niu, Tao; Liu, Huan; Tay, Franklin Chi Meng; Chen, Ji hua.

In: Oxidative medicine and cellular longevity, Vol. 2019, 3501059, 01.01.2019.

Research output: Contribution to journalArticle

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