Protective effect of Enicostemma Littorale blume methanolic extract on Gentamicin-induced nephrotoxicity in rats

Niraj Mukundray Bhatt, Kinjal Chauhan, Sharad Gupta, Prakash Pillai, Chirayu Pandya, Jyoti V. Thaikoottathil, Sarita S. Gupta

Research output: Contribution to journalArticle

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Abstract

Problem statement: The Amino glycoside antibiotics cause drug induced nephrotoxicity at therapeutic doses and thus limits the use clinically. Oxidative stress is one of the main reasons for the development of nephrotoxicity. Approach: In light of this the aim of the present study was to investigate the role of mitochondrial as well as post-mitochondrial oxidative stress in the development of Gentamicin (GM) induced nephrotoxicity and to elucidate the role of Enicostemma Littoral blame (EL) extract, as a promising antioxidant therapy. Rats were divided into four groups, 1) (Control 2) GM (i.p., 80 mg kg -1 day -1 for 8 days) (treated 3) GM+EL treated (i.g, 2.5 gm kg -1 day -1) and (4) GM+ Vitamin C (VC) treated (i.g, 600 mg kg -1 day -1). GM treated animals showed high oxidative stress in mitochondrial as well as post-mitochondrial fractions of renal tissues as evidenced by increased lipid per oxidation levels, decrease in GSH content and activities of antioxidant enzymes, SOD and GPx. Results: Oxidative stress was more pronounced in mitochondrial fraction as compared to post-mitochondrial fraction. GM-induced nephrotoxicity was further corroborated by an increase in serum cretonne and Blood Urea Nitrogen (BUN) levels and altered kidney histopathological observations. Treatment with EL ameliorates antioxidant defense system of mitochondrial as well as post-mitochondrial fraction, with better improvement seen in mitochondrial fraction. Conclusion: The present study explored beneficial effect of EL extract as an antioxidant therapy to counteract mitochondrial and post-mitochondrial oxidative stress generated in kidney upon GM-treatment, thus prevented nephrotoxicity.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalAmerican Journal of Infectious Diseases
Volume7
Issue number4
DOIs
StatePublished - Dec 1 2011

Fingerprint

Gentamicins
Oxidative Stress
Antioxidants
Kidney
Blood Urea Nitrogen
Glycosides
Ascorbic Acid
Therapeutics
Anti-Bacterial Agents
Lipids
Control Groups
Enzymes
Serum
Pharmaceutical Preparations

Keywords

  • Amino glycoside antibiotics
  • Antioxidant therapy
  • Enicostemma littoral blame
  • Lipid per oxidation
  • Mitochondrial fraction
  • Oxidative stress
  • Post-mitochondrial
  • Vitamin C (VC)

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Protective effect of Enicostemma Littorale blume methanolic extract on Gentamicin-induced nephrotoxicity in rats. / Bhatt, Niraj Mukundray; Chauhan, Kinjal; Gupta, Sharad; Pillai, Prakash; Pandya, Chirayu; Thaikoottathil, Jyoti V.; Gupta, Sarita S.

In: American Journal of Infectious Diseases, Vol. 7, No. 4, 01.12.2011, p. 83-90.

Research output: Contribution to journalArticle

Bhatt, Niraj Mukundray ; Chauhan, Kinjal ; Gupta, Sharad ; Pillai, Prakash ; Pandya, Chirayu ; Thaikoottathil, Jyoti V. ; Gupta, Sarita S. / Protective effect of Enicostemma Littorale blume methanolic extract on Gentamicin-induced nephrotoxicity in rats. In: American Journal of Infectious Diseases. 2011 ; Vol. 7, No. 4. pp. 83-90.
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abstract = "Problem statement: The Amino glycoside antibiotics cause drug induced nephrotoxicity at therapeutic doses and thus limits the use clinically. Oxidative stress is one of the main reasons for the development of nephrotoxicity. Approach: In light of this the aim of the present study was to investigate the role of mitochondrial as well as post-mitochondrial oxidative stress in the development of Gentamicin (GM) induced nephrotoxicity and to elucidate the role of Enicostemma Littoral blame (EL) extract, as a promising antioxidant therapy. Rats were divided into four groups, 1) (Control 2) GM (i.p., 80 mg kg -1 day -1 for 8 days) (treated 3) GM+EL treated (i.g, 2.5 gm kg -1 day -1) and (4) GM+ Vitamin C (VC) treated (i.g, 600 mg kg -1 day -1). GM treated animals showed high oxidative stress in mitochondrial as well as post-mitochondrial fractions of renal tissues as evidenced by increased lipid per oxidation levels, decrease in GSH content and activities of antioxidant enzymes, SOD and GPx. Results: Oxidative stress was more pronounced in mitochondrial fraction as compared to post-mitochondrial fraction. GM-induced nephrotoxicity was further corroborated by an increase in serum cretonne and Blood Urea Nitrogen (BUN) levels and altered kidney histopathological observations. Treatment with EL ameliorates antioxidant defense system of mitochondrial as well as post-mitochondrial fraction, with better improvement seen in mitochondrial fraction. Conclusion: The present study explored beneficial effect of EL extract as an antioxidant therapy to counteract mitochondrial and post-mitochondrial oxidative stress generated in kidney upon GM-treatment, thus prevented nephrotoxicity.",
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AU - Pillai, Prakash

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AU - Gupta, Sarita S.

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