Protective effect of green tea polyphenols on tributyltin-induced oxidative damage detected by in vivo and in vitro models

Huigang Liu, Zonglou Guo, Lihong Xu, Stephen Hsu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The current study investigated the protective effects of green tea polyphenols (GTPP) on TBT-induced oxidative damage. The results showed that reactive oxygen species (ROS) production and malondialdehyde content of the liver in mice exposed to TBT were reduced in the GTPP-treated group compared to the untreated group. The intracellular ROS level was elevated in TBT-treated human FL cells in a time-dependent manner. Comet assay data demonstrated that the number of cells with damaged DNA in untreated mice was found to be significantly higher compared to GTPP-treated mice. Damage to the nuclei and mitochondria observed in TBT-treated mice were alleviated in mice treated with both TBT and GTPP. The results represent the first observation that GTPP were effective in reducing TBT-induced oxidative damage both in vivo and in vitro. The possible protective mechanism may be due to the powerful ability of GTPP to scavenge ROS and prevent DNA breaks. We conclude that GTPP could be an effective agent or food supplement to reduce the cytotoxicity of TBT.

Original languageEnglish (US)
Pages (from-to)77-83
Number of pages7
JournalEnvironmental Toxicology
Volume23
Issue number1
DOIs
StatePublished - Feb 26 2008

Fingerprint

tributyltin
tea
Polyphenols
Tea
damage
Reactive Oxygen Species
Oxygen
DNA
Mitochondria
DNA Breaks
Comet Assay
mitochondrion
Cytotoxicity
Dietary Supplements
In Vitro Techniques
effect
Malondialdehyde
Liver
comet
Assays

Keywords

  • Green tea polyphenols
  • Oxidative damage
  • Protective effect
  • Tributyltin

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Protective effect of green tea polyphenols on tributyltin-induced oxidative damage detected by in vivo and in vitro models. / Liu, Huigang; Guo, Zonglou; Xu, Lihong; Hsu, Stephen.

In: Environmental Toxicology, Vol. 23, No. 1, 26.02.2008, p. 77-83.

Research output: Contribution to journalArticle

@article{39835fecc0f643a3a46cc036cc3d59e6,
title = "Protective effect of green tea polyphenols on tributyltin-induced oxidative damage detected by in vivo and in vitro models",
abstract = "The current study investigated the protective effects of green tea polyphenols (GTPP) on TBT-induced oxidative damage. The results showed that reactive oxygen species (ROS) production and malondialdehyde content of the liver in mice exposed to TBT were reduced in the GTPP-treated group compared to the untreated group. The intracellular ROS level was elevated in TBT-treated human FL cells in a time-dependent manner. Comet assay data demonstrated that the number of cells with damaged DNA in untreated mice was found to be significantly higher compared to GTPP-treated mice. Damage to the nuclei and mitochondria observed in TBT-treated mice were alleviated in mice treated with both TBT and GTPP. The results represent the first observation that GTPP were effective in reducing TBT-induced oxidative damage both in vivo and in vitro. The possible protective mechanism may be due to the powerful ability of GTPP to scavenge ROS and prevent DNA breaks. We conclude that GTPP could be an effective agent or food supplement to reduce the cytotoxicity of TBT.",
keywords = "Green tea polyphenols, Oxidative damage, Protective effect, Tributyltin",
author = "Huigang Liu and Zonglou Guo and Lihong Xu and Stephen Hsu",
year = "2008",
month = "2",
day = "26",
doi = "10.1002/tox.20312",
language = "English (US)",
volume = "23",
pages = "77--83",
journal = "Environmental Toxicology",
issn = "1520-4081",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Protective effect of green tea polyphenols on tributyltin-induced oxidative damage detected by in vivo and in vitro models

AU - Liu, Huigang

AU - Guo, Zonglou

AU - Xu, Lihong

AU - Hsu, Stephen

PY - 2008/2/26

Y1 - 2008/2/26

N2 - The current study investigated the protective effects of green tea polyphenols (GTPP) on TBT-induced oxidative damage. The results showed that reactive oxygen species (ROS) production and malondialdehyde content of the liver in mice exposed to TBT were reduced in the GTPP-treated group compared to the untreated group. The intracellular ROS level was elevated in TBT-treated human FL cells in a time-dependent manner. Comet assay data demonstrated that the number of cells with damaged DNA in untreated mice was found to be significantly higher compared to GTPP-treated mice. Damage to the nuclei and mitochondria observed in TBT-treated mice were alleviated in mice treated with both TBT and GTPP. The results represent the first observation that GTPP were effective in reducing TBT-induced oxidative damage both in vivo and in vitro. The possible protective mechanism may be due to the powerful ability of GTPP to scavenge ROS and prevent DNA breaks. We conclude that GTPP could be an effective agent or food supplement to reduce the cytotoxicity of TBT.

AB - The current study investigated the protective effects of green tea polyphenols (GTPP) on TBT-induced oxidative damage. The results showed that reactive oxygen species (ROS) production and malondialdehyde content of the liver in mice exposed to TBT were reduced in the GTPP-treated group compared to the untreated group. The intracellular ROS level was elevated in TBT-treated human FL cells in a time-dependent manner. Comet assay data demonstrated that the number of cells with damaged DNA in untreated mice was found to be significantly higher compared to GTPP-treated mice. Damage to the nuclei and mitochondria observed in TBT-treated mice were alleviated in mice treated with both TBT and GTPP. The results represent the first observation that GTPP were effective in reducing TBT-induced oxidative damage both in vivo and in vitro. The possible protective mechanism may be due to the powerful ability of GTPP to scavenge ROS and prevent DNA breaks. We conclude that GTPP could be an effective agent or food supplement to reduce the cytotoxicity of TBT.

KW - Green tea polyphenols

KW - Oxidative damage

KW - Protective effect

KW - Tributyltin

UR - http://www.scopus.com/inward/record.url?scp=39449098989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39449098989&partnerID=8YFLogxK

U2 - 10.1002/tox.20312

DO - 10.1002/tox.20312

M3 - Article

C2 - 18214927

AN - SCOPUS:39449098989

VL - 23

SP - 77

EP - 83

JO - Environmental Toxicology

JF - Environmental Toxicology

SN - 1520-4081

IS - 1

ER -