Protective effect of lipoic acid on micronuclei induction by cyclophosphamide

Elangovan Selvakumar, Chidambaram Prahalathan, Sudharsan Periyasamy Thandavan, Palaninathan Varalakshmi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present study investigated the protective efficacy of DL-α-lipoic acid on the cyclophosphamide (CP)-induced clastogenicity using the in vivo micronucleus assay. Male Wistar rats of 140 ± 20 g were categorized into eight groups. Five groups were administered CP (40 mg/kg body weight, intraperitonealy) to induce genotoxicity; four of these groups received a single intraperitoneal injection of lipoic acid at a dose of either 100 or 200 mg/kg body weight, and either 30 or 60 min prior to CP administration. A vehicle-treated control group and lipoic acid control groups were also included. The number of micronucleated polychromatic erythrocytes (MNPCEs) was determined at 24 h after CP administration. In rats injected with CP, the frequency of MNPCEs in bone marrow and peripheral blood was increased significantly in comparison with the controls, and in rats treated with lipoic acid and CP, the number of MNPCEs was decreased significantly in comparison to those given CP alone. The chemoprotective effect was found to be stronger after the administration of lipoic acid at a dose of 200 mg/kg body weight than 100 mg/kg body weight dosage, indicating the dose-dependent protective effect of lipoic acid. However, the protection by lipoic acid was not dependent on the time intervals between lipoic acid and CP administration. Our results illustrate the protective effect of lipoic acid on the in vivo clastogenicity induced by CP.

Original languageEnglish (US)
Pages (from-to)115-119
Number of pages5
JournalArchives of Toxicology
Volume80
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Thioctic Acid
Cyclophosphamide
Body Weight
Rats
Erythrocytes
Micronucleus Tests
Control Groups
Intraperitoneal Injections
Wistar Rats
Assays
Bone
Blood
Bone Marrow

Keywords

  • Bone marrow
  • Cyclophosphamide
  • Lipoic acid
  • Micronuclei
  • Peripheral blood

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Selvakumar, E., Prahalathan, C., Periyasamy Thandavan, S., & Varalakshmi, P. (2006). Protective effect of lipoic acid on micronuclei induction by cyclophosphamide. Archives of Toxicology, 80(2), 115-119. https://doi.org/10.1007/s00204-005-0015-7

Protective effect of lipoic acid on micronuclei induction by cyclophosphamide. / Selvakumar, Elangovan; Prahalathan, Chidambaram; Periyasamy Thandavan, Sudharsan; Varalakshmi, Palaninathan.

In: Archives of Toxicology, Vol. 80, No. 2, 01.02.2006, p. 115-119.

Research output: Contribution to journalArticle

Selvakumar, E, Prahalathan, C, Periyasamy Thandavan, S & Varalakshmi, P 2006, 'Protective effect of lipoic acid on micronuclei induction by cyclophosphamide', Archives of Toxicology, vol. 80, no. 2, pp. 115-119. https://doi.org/10.1007/s00204-005-0015-7
Selvakumar E, Prahalathan C, Periyasamy Thandavan S, Varalakshmi P. Protective effect of lipoic acid on micronuclei induction by cyclophosphamide. Archives of Toxicology. 2006 Feb 1;80(2):115-119. https://doi.org/10.1007/s00204-005-0015-7
Selvakumar, Elangovan ; Prahalathan, Chidambaram ; Periyasamy Thandavan, Sudharsan ; Varalakshmi, Palaninathan. / Protective effect of lipoic acid on micronuclei induction by cyclophosphamide. In: Archives of Toxicology. 2006 ; Vol. 80, No. 2. pp. 115-119.
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