Protective effects of EGCG on salivary gland cells treated with γ-radiation or cis-platinum(II) diammine dichloride

Tetsuya Yamamoto, Jared Staples, John Wataha, Jill Lewis, Petra Lockwood, Patricia V Schoenlein, Sushma Rao, Tokio Osaki, Douglas Dickinson, Takaaki Kamatani, George Schuster, Stephen Hsu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Dysfunction of salivary glands is often associated with aging and cancer therapy. Green tea polyphenols were previously found to protect normal epithelial cells from reactive oxygen species, and to induce apoptosis in tumor cells. The current study investigated whether -(-) epigallocatechin-3-gallate (EGCG), the major green tea polyphenol, protects normal salivary gland cells from the effects of γ-irradiation and the chemotherapy drug cis-platinum(II)diammine dichloride (CDDP). Human immortalized salivary acinar and ductal cells, and oral squamous cell carcinoma cells were irradiated with γ-rays or treated with CDDP, with or without pretreatment with EGCG, followed by MTT and BrdU incorporation assays. The results demonstrated that EGCG protected the normal salivary gland cells from chemical or irradiation-induced damage. However, protection of oral cancer cells by EGCG was also observed if EGCG was at physiologically achievable salivary concentrations but not at higher concentrations, suggesting that the combination of green tea consumption with cancer therapy requires further evaluation.

Original languageEnglish (US)
Pages (from-to)3065-3073
Number of pages9
JournalAnticancer Research
Volume24
Issue number5 A
StatePublished - Sep 1 2004

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Salivary Glands
Cisplatin
Radiation
Tea
Polyphenols
Neoplasms
Acinar Cells
Mouth Neoplasms
Bromodeoxyuridine
Squamous Cell Carcinoma
Reactive Oxygen Species
Epithelial Cells
epigallocatechin gallate
Apoptosis
Drug Therapy
Therapeutics
Pharmaceutical Preparations

Keywords

  • EGCG
  • Oral carcinomas
  • Salivary gland
  • Tea
  • Xerostomia

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Protective effects of EGCG on salivary gland cells treated with γ-radiation or cis-platinum(II) diammine dichloride. / Yamamoto, Tetsuya; Staples, Jared; Wataha, John; Lewis, Jill; Lockwood, Petra; Schoenlein, Patricia V; Rao, Sushma; Osaki, Tokio; Dickinson, Douglas; Kamatani, Takaaki; Schuster, George; Hsu, Stephen.

In: Anticancer Research, Vol. 24, No. 5 A, 01.09.2004, p. 3065-3073.

Research output: Contribution to journalArticle

Yamamoto, T, Staples, J, Wataha, J, Lewis, J, Lockwood, P, Schoenlein, PV, Rao, S, Osaki, T, Dickinson, D, Kamatani, T, Schuster, G & Hsu, S 2004, 'Protective effects of EGCG on salivary gland cells treated with γ-radiation or cis-platinum(II) diammine dichloride', Anticancer Research, vol. 24, no. 5 A, pp. 3065-3073.
Yamamoto, Tetsuya ; Staples, Jared ; Wataha, John ; Lewis, Jill ; Lockwood, Petra ; Schoenlein, Patricia V ; Rao, Sushma ; Osaki, Tokio ; Dickinson, Douglas ; Kamatani, Takaaki ; Schuster, George ; Hsu, Stephen. / Protective effects of EGCG on salivary gland cells treated with γ-radiation or cis-platinum(II) diammine dichloride. In: Anticancer Research. 2004 ; Vol. 24, No. 5 A. pp. 3065-3073.
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