Protective effects of EGCG on salivary gland cells treated with γ-radiation or cis-platinum(II) diammine dichloride

Tetsuya Yamamoto, Jared Staples, John Wataha, Jill Lewis, Petra Lockwood, Patricia Schoenlein, Sushma Rao, Tokio Osaki, Douglas Dickinson, Takaaki Kamatani, George Schuster, Stephen Hsu

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Dysfunction of salivary glands is often associated with aging and cancer therapy. Green tea polyphenols were previously found to protect normal epithelial cells from reactive oxygen species, and to induce apoptosis in tumor cells. The current study investigated whether -(-) epigallocatechin-3-gallate (EGCG), the major green tea polyphenol, protects normal salivary gland cells from the effects of γ-irradiation and the chemotherapy drug cis-platinum(II)diammine dichloride (CDDP). Human immortalized salivary acinar and ductal cells, and oral squamous cell carcinoma cells were irradiated with γ-rays or treated with CDDP, with or without pretreatment with EGCG, followed by MTT and BrdU incorporation assays. The results demonstrated that EGCG protected the normal salivary gland cells from chemical or irradiation-induced damage. However, protection of oral cancer cells by EGCG was also observed if EGCG was at physiologically achievable salivary concentrations but not at higher concentrations, suggesting that the combination of green tea consumption with cancer therapy requires further evaluation.

Original languageEnglish (US)
Pages (from-to)3065-3073
Number of pages9
JournalAnticancer research
Volume24
Issue number5 A
Publication statusPublished - Sep 1 2004

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Keywords

  • EGCG
  • Oral carcinomas
  • Salivary gland
  • Tea
  • Xerostomia

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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