Protective effects of ethanolic extract of Delphinium denudatum in a rat model of Parkinson's disease

Muzamil Ahmad, Seema Yousuf, Mohammad Badruzzaman Khan, Abdullah Shafique Ahmad, Sofiyan Saleem, Md Nasrul Hoda, Fakhrul Islam

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Parkinson's disease (FD) is one of the major neurodegenerative disorders, and oxidative stress has been implicated in playing an important role in the pathogenesis of the disease. In the present study, we investigated if Delphinium denudatum extract can slow down the neuronal injury in 6-hydroxydopamine (6-OHDA) rat model of Parkinsonism. Rats were treated with 200, 400 and 600 mg/kg body weight (b.w.) of D. denudatum extract for 3 weeks. On day 22, 2 μL of 6-OHDA (10 μg in 0.1% ascorbic acid-saline) or vehicle was infused into the right striatum of the animals. Three weeks after the 6-OHDA injections, the rats were killed for estimation of lipid peroxidation (LPO), reduced glutathione (GSH) content, superoxide dismutase (SOD) and catalase (CAT) activities, catecholamines, dopaminergic D2 receptor binding and tyrosine hydroxylase (TH) expression. Increased LPO and significant depletion of reduced GSH content in the substantia nigra resulting from the lesion were appreciably prevented with Delphinium treatment. Delphinium extract also dose-dependently attenuated the activities of SOD and CAT in striatum, which had been reduced significantly by lesioning. A significant decrease in the level of dopamine (DA) and its metabolites and an increase in the number of dopaminergic D2 receptors in striatum were observed after 6-OHDA injection, both parameters were significantly recovered with treatment of the extract. Finally, all these results were confirmed by an increase in expression of TH in the ipsilateral striatum of the lesioned groups following treatment with Delphinium extract. Thus, the study indicates that D. denudation extract may be helpful in checking neuronal injury in Parkinsonism.

Original languageEnglish (US)
Pages (from-to)361-368
Number of pages8
JournalHuman and Experimental Toxicology
Volume25
Issue number7
DOIs
StatePublished - Jul 1 2006

Fingerprint

Delphinium
Oxidopamine
Parkinson Disease
Rats
Tyrosine 3-Monooxygenase
Parkinsonian Disorders
Catalase
Lipid Peroxidation
Superoxide Dismutase
Animal Rights
Lipids
Injections
Oxidative stress
Wounds and Injuries
Substantia Nigra
Metabolites
Neurodegenerative Diseases
Ascorbic Acid
Catecholamines
Glutathione

Keywords

  • 6-hydroxydopamine
  • Anti-oxidant
  • Delphinium denudatum
  • Herbal drugs
  • Neuroprotection
  • Oxidative stress

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Protective effects of ethanolic extract of Delphinium denudatum in a rat model of Parkinson's disease. / Ahmad, Muzamil; Yousuf, Seema; Khan, Mohammad Badruzzaman; Ahmad, Abdullah Shafique; Saleem, Sofiyan; Hoda, Md Nasrul; Islam, Fakhrul.

In: Human and Experimental Toxicology, Vol. 25, No. 7, 01.07.2006, p. 361-368.

Research output: Contribution to journalArticle

Ahmad, Muzamil ; Yousuf, Seema ; Khan, Mohammad Badruzzaman ; Ahmad, Abdullah Shafique ; Saleem, Sofiyan ; Hoda, Md Nasrul ; Islam, Fakhrul. / Protective effects of ethanolic extract of Delphinium denudatum in a rat model of Parkinson's disease. In: Human and Experimental Toxicology. 2006 ; Vol. 25, No. 7. pp. 361-368.
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AU - Ahmad, Abdullah Shafique

AU - Saleem, Sofiyan

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AU - Islam, Fakhrul

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AB - Parkinson's disease (FD) is one of the major neurodegenerative disorders, and oxidative stress has been implicated in playing an important role in the pathogenesis of the disease. In the present study, we investigated if Delphinium denudatum extract can slow down the neuronal injury in 6-hydroxydopamine (6-OHDA) rat model of Parkinsonism. Rats were treated with 200, 400 and 600 mg/kg body weight (b.w.) of D. denudatum extract for 3 weeks. On day 22, 2 μL of 6-OHDA (10 μg in 0.1% ascorbic acid-saline) or vehicle was infused into the right striatum of the animals. Three weeks after the 6-OHDA injections, the rats were killed for estimation of lipid peroxidation (LPO), reduced glutathione (GSH) content, superoxide dismutase (SOD) and catalase (CAT) activities, catecholamines, dopaminergic D2 receptor binding and tyrosine hydroxylase (TH) expression. Increased LPO and significant depletion of reduced GSH content in the substantia nigra resulting from the lesion were appreciably prevented with Delphinium treatment. Delphinium extract also dose-dependently attenuated the activities of SOD and CAT in striatum, which had been reduced significantly by lesioning. A significant decrease in the level of dopamine (DA) and its metabolites and an increase in the number of dopaminergic D2 receptors in striatum were observed after 6-OHDA injection, both parameters were significantly recovered with treatment of the extract. Finally, all these results were confirmed by an increase in expression of TH in the ipsilateral striatum of the lesioned groups following treatment with Delphinium extract. Thus, the study indicates that D. denudation extract may be helpful in checking neuronal injury in Parkinsonism.

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