Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes

Bong Sook Jhun, Jin O-Uchi, Stephanie M. Adaniya, Thomas J. Mancini, Jessica L. Cao, Michelle E. King, Amy K. Landi, Hanley Ma, Milla Shin, Donqin Yang, Xiaole Xu, Yisang Yoon, Gaurav Choudhary, Richard T. Clements, Ulrike Mende, Shey Shing Sheu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Key points: Abnormal mitochondrial morphology and function in cardiomyocytes are frequently observed under persistent G q protein-coupled receptor (G q PCR) stimulation. Cardiac signalling mechanisms for regulating mitochondrial morphology and function under pathophysiological conditions in the heart are still poorly understood. We demonstrate that a downstream kinase of G q PCR, protein kinase D (PKD) induces mitochondrial fragmentation via phosphorylation of dynamin-like protein 1 (DLP1), a mitochondrial fission protein. The fragmented mitochondria enhance reactive oxygen species generation and permeability transition pore opening in mitochondria, which initiate apoptotic signalling activation. This study identifies a novel PKD-specific substrate in cardiac mitochondria and uncovers the role of PKD on cardiac mitochondria, with special emphasis on the molecular mechanism(s) underlying mitochondrial injury with abnormal mitochondrial morphology under persistent G q PCR stimulation. These findings provide new insights into the molecular basis of cardiac mitochondrial physiology and pathophysiology, linking G q PCR signalling with the regulation of mitochondrial morphology and function. Abstract: Regulation of mitochondrial morphology is crucial for the maintenance of physiological functions in many cell types including cardiomyocytes. Small and fragmented mitochondria are frequently observed in pathological conditions, but it is still unclear which cardiac signalling pathway is responsible for regulating the abnormal mitochondrial morphology in cardiomyocytes. Here we demonstrate that a downstream kinase of G q protein-coupled receptor (G q PCR) signalling, protein kinase D (PKD), mediates pathophysiological modifications in mitochondrial morphology and function, which consequently contribute to the activation of apoptotic signalling. We show that G q PCR stimulation induced by α 1 -adrenergic stimulation mediates mitochondrial fragmentation in a fission- and PKD-dependent manner in H9c2 cardiac myoblasts and rat neonatal cardiomyocytes. Upon G q PCR stimulation, PKD translocates from the cytoplasm to the outer mitochondrial membrane (OMM) and phosphorylates a mitochondrial fission protein, dynamin-like protein 1 (DLP1), at S637. PKD-dependent phosphorylation of DLP1 initiates DLP1 association with the OMM, which then enhances mitochondrial fragmentation, mitochondrial superoxide generation, mitochondrial permeability transition pore opening and apoptotic signalling. Finally, we demonstrate that DLP1 phosphorylation at S637 by PKD occurs in vivo using ventricular tissues from transgenic mice with cardiac-specific overexpression of constitutively active Gα q protein. In conclusion, G q PCR-PKD signalling induces mitochondrial fragmentation and dysfunction via PKD-dependent DLP1 phosphorylation in cardiomyocytes. This study is the first to identify a novel PKD-specific substrate, DLP1 in mitochondria, as well as the functional role of PKD in cardiac mitochondria. Elucidation of these molecular mechanisms by which PKD-dependent enhanced fission mediates cardiac mitochondrial injury will provide novel insight into the relationship among mitochondrial form, function and G q PCR signalling.

Original languageEnglish (US)
Pages (from-to)827-855
Number of pages29
JournalJournal of Physiology
Volume596
Issue number5
DOIs
StatePublished - Mar 1 2018

Fingerprint

Cardiac Myocytes
Dynamins
G-Protein-Coupled Receptors
Mitochondria
Proteins
G-Protein-Coupled Receptor Kinases
Phosphorylation
Mitochondrial Dynamics
Mitochondrial Proteins
Mitochondrial Membranes
protein kinase D
Cardiac Myoblasts
Dynamin I
Wounds and Injuries
Superoxides
Adrenergic Agents
Transgenic Mice
Permeability
Reactive Oxygen Species
Cytoplasm

Keywords

  • DRP1
  • ROS
  • adrenoceptor

ASJC Scopus subject areas

  • Physiology

Cite this

Jhun, B. S., O-Uchi, J., Adaniya, S. M., Mancini, T. J., Cao, J. L., King, M. E., ... Sheu, S. S. (2018). Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes. Journal of Physiology, 596(5), 827-855. https://doi.org/10.1113/JP275418

Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes. / Jhun, Bong Sook; O-Uchi, Jin; Adaniya, Stephanie M.; Mancini, Thomas J.; Cao, Jessica L.; King, Michelle E.; Landi, Amy K.; Ma, Hanley; Shin, Milla; Yang, Donqin; Xu, Xiaole; Yoon, Yisang; Choudhary, Gaurav; Clements, Richard T.; Mende, Ulrike; Sheu, Shey Shing.

In: Journal of Physiology, Vol. 596, No. 5, 01.03.2018, p. 827-855.

Research output: Contribution to journalArticle

Jhun, BS, O-Uchi, J, Adaniya, SM, Mancini, TJ, Cao, JL, King, ME, Landi, AK, Ma, H, Shin, M, Yang, D, Xu, X, Yoon, Y, Choudhary, G, Clements, RT, Mende, U & Sheu, SS 2018, 'Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes', Journal of Physiology, vol. 596, no. 5, pp. 827-855. https://doi.org/10.1113/JP275418
Jhun, Bong Sook ; O-Uchi, Jin ; Adaniya, Stephanie M. ; Mancini, Thomas J. ; Cao, Jessica L. ; King, Michelle E. ; Landi, Amy K. ; Ma, Hanley ; Shin, Milla ; Yang, Donqin ; Xu, Xiaole ; Yoon, Yisang ; Choudhary, Gaurav ; Clements, Richard T. ; Mende, Ulrike ; Sheu, Shey Shing. / Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes. In: Journal of Physiology. 2018 ; Vol. 596, No. 5. pp. 827-855.
@article{a364a944923b4cef8a0304843b2c042e,
title = "Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes",
abstract = "Key points: Abnormal mitochondrial morphology and function in cardiomyocytes are frequently observed under persistent G q protein-coupled receptor (G q PCR) stimulation. Cardiac signalling mechanisms for regulating mitochondrial morphology and function under pathophysiological conditions in the heart are still poorly understood. We demonstrate that a downstream kinase of G q PCR, protein kinase D (PKD) induces mitochondrial fragmentation via phosphorylation of dynamin-like protein 1 (DLP1), a mitochondrial fission protein. The fragmented mitochondria enhance reactive oxygen species generation and permeability transition pore opening in mitochondria, which initiate apoptotic signalling activation. This study identifies a novel PKD-specific substrate in cardiac mitochondria and uncovers the role of PKD on cardiac mitochondria, with special emphasis on the molecular mechanism(s) underlying mitochondrial injury with abnormal mitochondrial morphology under persistent G q PCR stimulation. These findings provide new insights into the molecular basis of cardiac mitochondrial physiology and pathophysiology, linking G q PCR signalling with the regulation of mitochondrial morphology and function. Abstract: Regulation of mitochondrial morphology is crucial for the maintenance of physiological functions in many cell types including cardiomyocytes. Small and fragmented mitochondria are frequently observed in pathological conditions, but it is still unclear which cardiac signalling pathway is responsible for regulating the abnormal mitochondrial morphology in cardiomyocytes. Here we demonstrate that a downstream kinase of G q protein-coupled receptor (G q PCR) signalling, protein kinase D (PKD), mediates pathophysiological modifications in mitochondrial morphology and function, which consequently contribute to the activation of apoptotic signalling. We show that G q PCR stimulation induced by α 1 -adrenergic stimulation mediates mitochondrial fragmentation in a fission- and PKD-dependent manner in H9c2 cardiac myoblasts and rat neonatal cardiomyocytes. Upon G q PCR stimulation, PKD translocates from the cytoplasm to the outer mitochondrial membrane (OMM) and phosphorylates a mitochondrial fission protein, dynamin-like protein 1 (DLP1), at S637. PKD-dependent phosphorylation of DLP1 initiates DLP1 association with the OMM, which then enhances mitochondrial fragmentation, mitochondrial superoxide generation, mitochondrial permeability transition pore opening and apoptotic signalling. Finally, we demonstrate that DLP1 phosphorylation at S637 by PKD occurs in vivo using ventricular tissues from transgenic mice with cardiac-specific overexpression of constitutively active Gα q protein. In conclusion, G q PCR-PKD signalling induces mitochondrial fragmentation and dysfunction via PKD-dependent DLP1 phosphorylation in cardiomyocytes. This study is the first to identify a novel PKD-specific substrate, DLP1 in mitochondria, as well as the functional role of PKD in cardiac mitochondria. Elucidation of these molecular mechanisms by which PKD-dependent enhanced fission mediates cardiac mitochondrial injury will provide novel insight into the relationship among mitochondrial form, function and G q PCR signalling.",
keywords = "DRP1, ROS, adrenoceptor",
author = "Jhun, {Bong Sook} and Jin O-Uchi and Adaniya, {Stephanie M.} and Mancini, {Thomas J.} and Cao, {Jessica L.} and King, {Michelle E.} and Landi, {Amy K.} and Hanley Ma and Milla Shin and Donqin Yang and Xiaole Xu and Yisang Yoon and Gaurav Choudhary and Clements, {Richard T.} and Ulrike Mende and Sheu, {Shey Shing}",
year = "2018",
month = "3",
day = "1",
doi = "10.1113/JP275418",
language = "English (US)",
volume = "596",
pages = "827--855",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes

AU - Jhun, Bong Sook

AU - O-Uchi, Jin

AU - Adaniya, Stephanie M.

AU - Mancini, Thomas J.

AU - Cao, Jessica L.

AU - King, Michelle E.

AU - Landi, Amy K.

AU - Ma, Hanley

AU - Shin, Milla

AU - Yang, Donqin

AU - Xu, Xiaole

AU - Yoon, Yisang

AU - Choudhary, Gaurav

AU - Clements, Richard T.

AU - Mende, Ulrike

AU - Sheu, Shey Shing

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Key points: Abnormal mitochondrial morphology and function in cardiomyocytes are frequently observed under persistent G q protein-coupled receptor (G q PCR) stimulation. Cardiac signalling mechanisms for regulating mitochondrial morphology and function under pathophysiological conditions in the heart are still poorly understood. We demonstrate that a downstream kinase of G q PCR, protein kinase D (PKD) induces mitochondrial fragmentation via phosphorylation of dynamin-like protein 1 (DLP1), a mitochondrial fission protein. The fragmented mitochondria enhance reactive oxygen species generation and permeability transition pore opening in mitochondria, which initiate apoptotic signalling activation. This study identifies a novel PKD-specific substrate in cardiac mitochondria and uncovers the role of PKD on cardiac mitochondria, with special emphasis on the molecular mechanism(s) underlying mitochondrial injury with abnormal mitochondrial morphology under persistent G q PCR stimulation. These findings provide new insights into the molecular basis of cardiac mitochondrial physiology and pathophysiology, linking G q PCR signalling with the regulation of mitochondrial morphology and function. Abstract: Regulation of mitochondrial morphology is crucial for the maintenance of physiological functions in many cell types including cardiomyocytes. Small and fragmented mitochondria are frequently observed in pathological conditions, but it is still unclear which cardiac signalling pathway is responsible for regulating the abnormal mitochondrial morphology in cardiomyocytes. Here we demonstrate that a downstream kinase of G q protein-coupled receptor (G q PCR) signalling, protein kinase D (PKD), mediates pathophysiological modifications in mitochondrial morphology and function, which consequently contribute to the activation of apoptotic signalling. We show that G q PCR stimulation induced by α 1 -adrenergic stimulation mediates mitochondrial fragmentation in a fission- and PKD-dependent manner in H9c2 cardiac myoblasts and rat neonatal cardiomyocytes. Upon G q PCR stimulation, PKD translocates from the cytoplasm to the outer mitochondrial membrane (OMM) and phosphorylates a mitochondrial fission protein, dynamin-like protein 1 (DLP1), at S637. PKD-dependent phosphorylation of DLP1 initiates DLP1 association with the OMM, which then enhances mitochondrial fragmentation, mitochondrial superoxide generation, mitochondrial permeability transition pore opening and apoptotic signalling. Finally, we demonstrate that DLP1 phosphorylation at S637 by PKD occurs in vivo using ventricular tissues from transgenic mice with cardiac-specific overexpression of constitutively active Gα q protein. In conclusion, G q PCR-PKD signalling induces mitochondrial fragmentation and dysfunction via PKD-dependent DLP1 phosphorylation in cardiomyocytes. This study is the first to identify a novel PKD-specific substrate, DLP1 in mitochondria, as well as the functional role of PKD in cardiac mitochondria. Elucidation of these molecular mechanisms by which PKD-dependent enhanced fission mediates cardiac mitochondrial injury will provide novel insight into the relationship among mitochondrial form, function and G q PCR signalling.

AB - Key points: Abnormal mitochondrial morphology and function in cardiomyocytes are frequently observed under persistent G q protein-coupled receptor (G q PCR) stimulation. Cardiac signalling mechanisms for regulating mitochondrial morphology and function under pathophysiological conditions in the heart are still poorly understood. We demonstrate that a downstream kinase of G q PCR, protein kinase D (PKD) induces mitochondrial fragmentation via phosphorylation of dynamin-like protein 1 (DLP1), a mitochondrial fission protein. The fragmented mitochondria enhance reactive oxygen species generation and permeability transition pore opening in mitochondria, which initiate apoptotic signalling activation. This study identifies a novel PKD-specific substrate in cardiac mitochondria and uncovers the role of PKD on cardiac mitochondria, with special emphasis on the molecular mechanism(s) underlying mitochondrial injury with abnormal mitochondrial morphology under persistent G q PCR stimulation. These findings provide new insights into the molecular basis of cardiac mitochondrial physiology and pathophysiology, linking G q PCR signalling with the regulation of mitochondrial morphology and function. Abstract: Regulation of mitochondrial morphology is crucial for the maintenance of physiological functions in many cell types including cardiomyocytes. Small and fragmented mitochondria are frequently observed in pathological conditions, but it is still unclear which cardiac signalling pathway is responsible for regulating the abnormal mitochondrial morphology in cardiomyocytes. Here we demonstrate that a downstream kinase of G q protein-coupled receptor (G q PCR) signalling, protein kinase D (PKD), mediates pathophysiological modifications in mitochondrial morphology and function, which consequently contribute to the activation of apoptotic signalling. We show that G q PCR stimulation induced by α 1 -adrenergic stimulation mediates mitochondrial fragmentation in a fission- and PKD-dependent manner in H9c2 cardiac myoblasts and rat neonatal cardiomyocytes. Upon G q PCR stimulation, PKD translocates from the cytoplasm to the outer mitochondrial membrane (OMM) and phosphorylates a mitochondrial fission protein, dynamin-like protein 1 (DLP1), at S637. PKD-dependent phosphorylation of DLP1 initiates DLP1 association with the OMM, which then enhances mitochondrial fragmentation, mitochondrial superoxide generation, mitochondrial permeability transition pore opening and apoptotic signalling. Finally, we demonstrate that DLP1 phosphorylation at S637 by PKD occurs in vivo using ventricular tissues from transgenic mice with cardiac-specific overexpression of constitutively active Gα q protein. In conclusion, G q PCR-PKD signalling induces mitochondrial fragmentation and dysfunction via PKD-dependent DLP1 phosphorylation in cardiomyocytes. This study is the first to identify a novel PKD-specific substrate, DLP1 in mitochondria, as well as the functional role of PKD in cardiac mitochondria. Elucidation of these molecular mechanisms by which PKD-dependent enhanced fission mediates cardiac mitochondrial injury will provide novel insight into the relationship among mitochondrial form, function and G q PCR signalling.

KW - DRP1

KW - ROS

KW - adrenoceptor

UR - http://www.scopus.com/inward/record.url?scp=85041028222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041028222&partnerID=8YFLogxK

U2 - 10.1113/JP275418

DO - 10.1113/JP275418

M3 - Article

C2 - 29313986

AN - SCOPUS:85041028222

VL - 596

SP - 827

EP - 855

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 5

ER -