Protein microarrays to detect protein-protein interactions using red and green fluorescent proteins

Thomas Kukar, Sarah Eckenrode, Yunrong Gu, Wei Lian, Mike Megginson, Jin Xiong She, Donghai Wu

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Proteomics, the study of protein function on a global scale, will play an important role in furthering our understanding of gene functions, complex biological pathways, and discovery of novel drug targets. A number of techniques have been developed for proteomic studies to identify and analyze proteins, compare protein expression levels, and study protein-protein interactions. Recent developments have applied a DNA array-type approach to immobilize proteins on a surface for high-throughput analysis. Here we report the development and construction of protein chips using derivatized glass and nitrocellulose-coated slides and the employment of recombinant proteins fused with green and red fluorescent proteins for detection. Fluorescent signals were found to be proportional to the amount of arrayed proteins and could be readily detected with a conventional fluorescence slide scanner. This technique allows the investigation of protein-protein interactions without the need for additional labeling steps of probe proteins.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalAnalytical Biochemistry
Volume306
Issue number1
DOIs
StatePublished - Jul 1 2002

Keywords

  • Fluorescent proteins
  • Green fluorescent protein
  • Microarrays
  • Protein interactions
  • Red fluorescent protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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