Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress

Dehuang Guo, Junyan Han, Bao Ling Adam, Nancy H. Colburn, Mong-Heng Wang, Zheng Dong, Decio L. Eizirik, Jin-Xiong She, Cong Yi Wang

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The substrates of SUMO4, a novel member for the SUMO gene family, were characterized in HEK293 cells cultured under serum starvation by proteomic analysis. We identified 90 SUMO4 substrates including anti-stress proteins such as antioxidant enzymes and molecular chaperones or co-chaperones. The substrates also include proteins involved in the regulation of DNA repair and synthesis, RNA processing, protein degradation, and glucose metabolism. Several SUMO4-associated transcription factors were characterized by Western blot analyses. AP-1 was selected for in vitro conjugation assays to confirm SUMO4 sumoylation of these transcription factors. Further functional analyses of the transcription factors suggested that SUMO4 sumoylation represses AP-1 and AP-2α transcriptional activity, but enhances GR DNA binding capacity. These results demonstrate that SUMO4 sumoylation may play an important role in the regulation of intracellular stress.

Original languageEnglish (US)
Pages (from-to)1308-1318
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number4
DOIs
StatePublished - Dec 2 2005

Fingerprint

Sumoylation
HEK293 Cells
Starvation
Proteomics
Transcription Factors
Transcription Factor AP-1
Substrates
Serum
Molecular Chaperones
DNA
Heat-Shock Proteins
Metabolism
DNA Repair
Proteolysis
Assays
Proteins
Repair
Antioxidants
Genes
Western Blotting

Keywords

  • 2D PAGE
  • HO
  • MALDI-TOF
  • ROS
  • SUMO
  • Stress
  • Substrate
  • Sumoylation
  • Ubiquitylation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress. / Guo, Dehuang; Han, Junyan; Adam, Bao Ling; Colburn, Nancy H.; Wang, Mong-Heng; Dong, Zheng; Eizirik, Decio L.; She, Jin-Xiong; Wang, Cong Yi.

In: Biochemical and Biophysical Research Communications, Vol. 337, No. 4, 02.12.2005, p. 1308-1318.

Research output: Contribution to journalArticle

Guo, Dehuang ; Han, Junyan ; Adam, Bao Ling ; Colburn, Nancy H. ; Wang, Mong-Heng ; Dong, Zheng ; Eizirik, Decio L. ; She, Jin-Xiong ; Wang, Cong Yi. / Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 337, No. 4. pp. 1308-1318.
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