Abstract
The substrates of SUMO4, a novel member for the SUMO gene family, were characterized in HEK293 cells cultured under serum starvation by proteomic analysis. We identified 90 SUMO4 substrates including anti-stress proteins such as antioxidant enzymes and molecular chaperones or co-chaperones. The substrates also include proteins involved in the regulation of DNA repair and synthesis, RNA processing, protein degradation, and glucose metabolism. Several SUMO4-associated transcription factors were characterized by Western blot analyses. AP-1 was selected for in vitro conjugation assays to confirm SUMO4 sumoylation of these transcription factors. Further functional analyses of the transcription factors suggested that SUMO4 sumoylation represses AP-1 and AP-2α transcriptional activity, but enhances GR DNA binding capacity. These results demonstrate that SUMO4 sumoylation may play an important role in the regulation of intracellular stress.
Original language | English (US) |
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Pages (from-to) | 1308-1318 |
Number of pages | 11 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 337 |
Issue number | 4 |
DOIs | |
State | Published - Dec 2 2005 |
Keywords
- 2D PAGE
- HO
- MALDI-TOF
- ROS
- SUMO
- Stress
- Substrate
- Sumoylation
- Ubiquitylation
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology