Proteomic analysis of tyrosine phosphorylations in vascular endothelial growth factor- and reactive oxygen species-mediated signaling pathway

Young-Mee Kim, Joo Song Eun, Jawon Seo, Hee Jung Kim, Kong Joo Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) mediates angiogenic signaling by activating tyrosine kinase receptors. Endothelial cells treated with VEGF are known to increase reactive oxygen species (ROS) production and activate the MAPK pathway. To identify the target proteins of the VEGF receptor, we treated human umbilical vein endothelial cells (HUVECs) with VEGF or H2O 2, and identified and semiquantified tyrosine-phosphorylated proteins, combining 2D-gel electrophoresis, Western analysis using antibody against phospho-tyrosine, and mass spectrometry. We detected 95 proteins that were differentially phosphorylated; some were specifically phosphorylated by VEGF but not by H2O2. 2D-gel electrophoresis revealed that heterogeneous populations of the same protein responded differently to H 2O2 and VEGF. Bioinformatic studies examining the nature of the differential phosphorylation in various subpopulations of proteins should provide new insights into VEGF- and H2O2-induced signaling pathways.

Original languageEnglish (US)
Pages (from-to)593-601
Number of pages9
JournalJournal of Proteome Research
Volume6
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Phosphorylation
Proteomics
Vascular Endothelial Growth Factor A
Tyrosine
Reactive Oxygen Species
Endothelial cells
Electrophoresis, Gel, Two-Dimensional
Proteins
Electrophoresis
Gels
Vascular Endothelial Growth Factor Receptor
Human Umbilical Vein Endothelial Cells
Receptor Protein-Tyrosine Kinases
Bioinformatics
Computational Biology
Mass spectrometry
Mass Spectrometry
Endothelial Cells
Antibodies
Population

Keywords

  • 2D-gel electrophoresis
  • HUVECs
  • Mass spectrometry
  • Proteomic analysis
  • ROS
  • Tubule formation
  • Tyrosine phosphorylation
  • VEGF

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Proteomic analysis of tyrosine phosphorylations in vascular endothelial growth factor- and reactive oxygen species-mediated signaling pathway. / Kim, Young-Mee; Eun, Joo Song; Seo, Jawon; Kim, Hee Jung; Lee, Kong Joo.

In: Journal of Proteome Research, Vol. 6, No. 2, 01.02.2007, p. 593-601.

Research output: Contribution to journalArticle

Kim, Young-Mee ; Eun, Joo Song ; Seo, Jawon ; Kim, Hee Jung ; Lee, Kong Joo. / Proteomic analysis of tyrosine phosphorylations in vascular endothelial growth factor- and reactive oxygen species-mediated signaling pathway. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 2. pp. 593-601.
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