Proteomic Mapping of Dental Enamel Matrix from Inbred Mouse Strains: Unraveling Potential New Players in Enamel

Aline Lima Leite, Mileni Silva Fernandes, Senda Charone, Gary M. Whitford, Eric T. Everett, Marília Afonso Rabelo Buzalaf

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Enamel formation is a complex 2-step process by which proteins are secreted to form an extracellular matrix, followed by massive protein degradation and subsequent mineralization. Excessive systemic exposure to fluoride can disrupt this process and lead to a condition known as dental fluorosis. The genetic background influences the responses of mineralized tissues to fluoride, such as dental fluorosis, observed in A/J and 129P3/J mice. The aim of the present study was to map the protein profile of enamel matrix from A/J and 129P3/J strains. Enamel matrix samples were obtained from A/J and 129P3/J mice and analyzed by 2-dimensional electrophoresis and liquid chromatography coupled with mass spectrometry. A total of 120 proteins were identified, and 7 f them were classified as putative uncharacterized proteins and analyzed in silico for structural and functional characterization. An interesting finding was the possibility of the uncharacterized sequence Q8BIS2 being an enzyme involved in the degradation of matrix proteins. Thus, the results provide a comprehensive view of the structure and function for putative uncharacterized proteins found in the enamel matrix that could help to elucidate the mechanisms involved in enamel biomineralization and genetic susceptibility to dental fluorosis.

Original languageEnglish (US)
Pages (from-to)78-87
Number of pages10
JournalCaries Research
Volume52
Issue number1-2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Inbred Strains Mice
Dental Fluorosis
Dental Enamel
Proteomics
Fluorides
Proteolysis
Proteins
Genetic Predisposition to Disease
Liquid Chromatography
Computer Simulation
Extracellular Matrix
Electrophoresis
Mass Spectrometry
Enzymes

Keywords

  • Amelogenesis
  • Biomineralization
  • Fluorosis
  • Proteomic analysis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Proteomic Mapping of Dental Enamel Matrix from Inbred Mouse Strains : Unraveling Potential New Players in Enamel. / Leite, Aline Lima; Silva Fernandes, Mileni; Charone, Senda; Whitford, Gary M.; Everett, Eric T.; Buzalaf, Marília Afonso Rabelo.

In: Caries Research, Vol. 52, No. 1-2, 01.02.2018, p. 78-87.

Research output: Contribution to journalArticle

Leite, Aline Lima ; Silva Fernandes, Mileni ; Charone, Senda ; Whitford, Gary M. ; Everett, Eric T. ; Buzalaf, Marília Afonso Rabelo. / Proteomic Mapping of Dental Enamel Matrix from Inbred Mouse Strains : Unraveling Potential New Players in Enamel. In: Caries Research. 2018 ; Vol. 52, No. 1-2. pp. 78-87.
@article{1513806809d74260b75bf8f661e739ef,
title = "Proteomic Mapping of Dental Enamel Matrix from Inbred Mouse Strains: Unraveling Potential New Players in Enamel",
abstract = "Enamel formation is a complex 2-step process by which proteins are secreted to form an extracellular matrix, followed by massive protein degradation and subsequent mineralization. Excessive systemic exposure to fluoride can disrupt this process and lead to a condition known as dental fluorosis. The genetic background influences the responses of mineralized tissues to fluoride, such as dental fluorosis, observed in A/J and 129P3/J mice. The aim of the present study was to map the protein profile of enamel matrix from A/J and 129P3/J strains. Enamel matrix samples were obtained from A/J and 129P3/J mice and analyzed by 2-dimensional electrophoresis and liquid chromatography coupled with mass spectrometry. A total of 120 proteins were identified, and 7 f them were classified as putative uncharacterized proteins and analyzed in silico for structural and functional characterization. An interesting finding was the possibility of the uncharacterized sequence Q8BIS2 being an enzyme involved in the degradation of matrix proteins. Thus, the results provide a comprehensive view of the structure and function for putative uncharacterized proteins found in the enamel matrix that could help to elucidate the mechanisms involved in enamel biomineralization and genetic susceptibility to dental fluorosis.",
keywords = "Amelogenesis, Biomineralization, Fluorosis, Proteomic analysis",
author = "Leite, {Aline Lima} and {Silva Fernandes}, Mileni and Senda Charone and Whitford, {Gary M.} and Everett, {Eric T.} and Buzalaf, {Mar{\'i}lia Afonso Rabelo}",
year = "2018",
month = "2",
day = "1",
doi = "10.1159/000479039",
language = "English (US)",
volume = "52",
pages = "78--87",
journal = "Caries Research",
issn = "0008-6568",
publisher = "S. Karger AG",
number = "1-2",

}

TY - JOUR

T1 - Proteomic Mapping of Dental Enamel Matrix from Inbred Mouse Strains

T2 - Unraveling Potential New Players in Enamel

AU - Leite, Aline Lima

AU - Silva Fernandes, Mileni

AU - Charone, Senda

AU - Whitford, Gary M.

AU - Everett, Eric T.

AU - Buzalaf, Marília Afonso Rabelo

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Enamel formation is a complex 2-step process by which proteins are secreted to form an extracellular matrix, followed by massive protein degradation and subsequent mineralization. Excessive systemic exposure to fluoride can disrupt this process and lead to a condition known as dental fluorosis. The genetic background influences the responses of mineralized tissues to fluoride, such as dental fluorosis, observed in A/J and 129P3/J mice. The aim of the present study was to map the protein profile of enamel matrix from A/J and 129P3/J strains. Enamel matrix samples were obtained from A/J and 129P3/J mice and analyzed by 2-dimensional electrophoresis and liquid chromatography coupled with mass spectrometry. A total of 120 proteins were identified, and 7 f them were classified as putative uncharacterized proteins and analyzed in silico for structural and functional characterization. An interesting finding was the possibility of the uncharacterized sequence Q8BIS2 being an enzyme involved in the degradation of matrix proteins. Thus, the results provide a comprehensive view of the structure and function for putative uncharacterized proteins found in the enamel matrix that could help to elucidate the mechanisms involved in enamel biomineralization and genetic susceptibility to dental fluorosis.

AB - Enamel formation is a complex 2-step process by which proteins are secreted to form an extracellular matrix, followed by massive protein degradation and subsequent mineralization. Excessive systemic exposure to fluoride can disrupt this process and lead to a condition known as dental fluorosis. The genetic background influences the responses of mineralized tissues to fluoride, such as dental fluorosis, observed in A/J and 129P3/J mice. The aim of the present study was to map the protein profile of enamel matrix from A/J and 129P3/J strains. Enamel matrix samples were obtained from A/J and 129P3/J mice and analyzed by 2-dimensional electrophoresis and liquid chromatography coupled with mass spectrometry. A total of 120 proteins were identified, and 7 f them were classified as putative uncharacterized proteins and analyzed in silico for structural and functional characterization. An interesting finding was the possibility of the uncharacterized sequence Q8BIS2 being an enzyme involved in the degradation of matrix proteins. Thus, the results provide a comprehensive view of the structure and function for putative uncharacterized proteins found in the enamel matrix that could help to elucidate the mechanisms involved in enamel biomineralization and genetic susceptibility to dental fluorosis.

KW - Amelogenesis

KW - Biomineralization

KW - Fluorosis

KW - Proteomic analysis

UR - http://www.scopus.com/inward/record.url?scp=85038623876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038623876&partnerID=8YFLogxK

U2 - 10.1159/000479039

DO - 10.1159/000479039

M3 - Article

C2 - 29248934

AN - SCOPUS:85038623876

VL - 52

SP - 78

EP - 87

JO - Caries Research

JF - Caries Research

SN - 0008-6568

IS - 1-2

ER -