Proteomic studies of the intrinsically unstructured mammalian proteome

Charles A. Galea, Vishwajeeth R. Pagala, John C. Obenauer, Cheon Gil Park, Clive A. Slaughter, Richard W. Kriwacki

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 °C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.

Original languageEnglish (US)
Pages (from-to)2839-2848
Number of pages10
JournalJournal of Proteome Research
Volume5
Issue number10
DOIs
StatePublished - Oct 1 2006

Fingerprint

Intrinsically Disordered Proteins
Proteome
Proteomics
Cell signaling
Fibroblasts
Nuclear Proteins
Cell Extracts
Hot Temperature
Heat treatment
Cells
Proteins

Keywords

  • Disordered proteins
  • Heat denaturation
  • Intrinsically unstructured proteins
  • Mammalian proteome
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Galea, C. A., Pagala, V. R., Obenauer, J. C., Park, C. G., Slaughter, C. A., & Kriwacki, R. W. (2006). Proteomic studies of the intrinsically unstructured mammalian proteome. Journal of Proteome Research, 5(10), 2839-2848. https://doi.org/10.1021/pr060328c

Proteomic studies of the intrinsically unstructured mammalian proteome. / Galea, Charles A.; Pagala, Vishwajeeth R.; Obenauer, John C.; Park, Cheon Gil; Slaughter, Clive A.; Kriwacki, Richard W.

In: Journal of Proteome Research, Vol. 5, No. 10, 01.10.2006, p. 2839-2848.

Research output: Contribution to journalArticle

Galea, CA, Pagala, VR, Obenauer, JC, Park, CG, Slaughter, CA & Kriwacki, RW 2006, 'Proteomic studies of the intrinsically unstructured mammalian proteome', Journal of Proteome Research, vol. 5, no. 10, pp. 2839-2848. https://doi.org/10.1021/pr060328c
Galea, Charles A. ; Pagala, Vishwajeeth R. ; Obenauer, John C. ; Park, Cheon Gil ; Slaughter, Clive A. ; Kriwacki, Richard W. / Proteomic studies of the intrinsically unstructured mammalian proteome. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 10. pp. 2839-2848.
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