Proteomics blood testing to distinguish chronic rhinosinusitis subtypes

Subinoy Das, Patricia A. Maeso, Adam M. Becker, John Drew Prosser, Bao Ling Adam, Stilianos E Kountakis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objectives: To evaluate the potential of surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) proteomic profiling of serum samples to distinguish chronic rhinosinusitis subtypes. Study Design: Translational study of serum samples from prospectively enrolled patients undergoing sinus surgery. Methods: Patients undergoing endoscopic sinus surgery for chronic rhinosinusitis with nasal polyposis were prospectively enrolled in an ongoing, institutional review board approved proteomics study. SELDI-TOF-MS was performed on 42 serum samples in patients with chronic rhinosinusitis with nasal polyposis (15 patients diagnosed with allergic fungal rhinosinusitis, 10 patients with Samter's triad, and 17 with chronic rhinosinusitis with nasal polyposis). Classification tree analysis on protein spectra developed from peaks detected in the 0 to 100 kD range was performed to identify disease subtypes. Results: SELDI-TOF-MS correctly identified patients with allergic fungal rhinosinusitis from serum samples with 84% sensitivity and 90% specificity, and correctly identified patients with Samter's triad with 88% sensitivity and 88% specificity in two subtype comparison groups. SELDI-TOF-MS correctly identified patients with allergic fungal rhinosinusitis with 76% sensitivity and 82% specificity, and correctly identified patients with Samter's triad with 80% sensitivity and 90% specificity in three subtype comparison groups. Conclusion: The study provides molecular evidence that allergic fungal rhinosinusitis is a discrete subtype of chronic rhinosinusitis. SELDI-TOF-MS is a promising technology that could lead to the development of a rapid blood test, to identify severe chronic rhinosinusitis subtypes. Further investigation into the utility of this technology is warranted.

Original languageEnglish (US)
Pages (from-to)2231-2234
Number of pages4
JournalLaryngoscope
Volume118
Issue number12
DOIs
StatePublished - Dec 1 2008

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Proteomics
Mass Spectrometry
Lasers
Nose
Sensitivity and Specificity
Serum
Technology
Research Ethics Committees
Hematologic Tests

Keywords

  • Allergic fungal rhinosinusitis
  • Chronic rhinosinusitis
  • Chronic rhinosinusitis with nasal polyposis
  • Proteomics
  • SELDI-TOF-MS
  • Samter's triad

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Proteomics blood testing to distinguish chronic rhinosinusitis subtypes. / Das, Subinoy; Maeso, Patricia A.; Becker, Adam M.; Prosser, John Drew; Adam, Bao Ling; Kountakis, Stilianos E.

In: Laryngoscope, Vol. 118, No. 12, 01.12.2008, p. 2231-2234.

Research output: Contribution to journalArticle

Das, Subinoy ; Maeso, Patricia A. ; Becker, Adam M. ; Prosser, John Drew ; Adam, Bao Ling ; Kountakis, Stilianos E. / Proteomics blood testing to distinguish chronic rhinosinusitis subtypes. In: Laryngoscope. 2008 ; Vol. 118, No. 12. pp. 2231-2234.
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abstract = "Objectives: To evaluate the potential of surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) proteomic profiling of serum samples to distinguish chronic rhinosinusitis subtypes. Study Design: Translational study of serum samples from prospectively enrolled patients undergoing sinus surgery. Methods: Patients undergoing endoscopic sinus surgery for chronic rhinosinusitis with nasal polyposis were prospectively enrolled in an ongoing, institutional review board approved proteomics study. SELDI-TOF-MS was performed on 42 serum samples in patients with chronic rhinosinusitis with nasal polyposis (15 patients diagnosed with allergic fungal rhinosinusitis, 10 patients with Samter's triad, and 17 with chronic rhinosinusitis with nasal polyposis). Classification tree analysis on protein spectra developed from peaks detected in the 0 to 100 kD range was performed to identify disease subtypes. Results: SELDI-TOF-MS correctly identified patients with allergic fungal rhinosinusitis from serum samples with 84{\%} sensitivity and 90{\%} specificity, and correctly identified patients with Samter's triad with 88{\%} sensitivity and 88{\%} specificity in two subtype comparison groups. SELDI-TOF-MS correctly identified patients with allergic fungal rhinosinusitis with 76{\%} sensitivity and 82{\%} specificity, and correctly identified patients with Samter's triad with 80{\%} sensitivity and 90{\%} specificity in three subtype comparison groups. Conclusion: The study provides molecular evidence that allergic fungal rhinosinusitis is a discrete subtype of chronic rhinosinusitis. SELDI-TOF-MS is a promising technology that could lead to the development of a rapid blood test, to identify severe chronic rhinosinusitis subtypes. Further investigation into the utility of this technology is warranted.",
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